Introduction to Fungal Proteinases and Expression in Fungal Systems

A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides

1983 • 1,143 citations

Developments in Industrial Microbiology

1960 • 423 citations

Structure and refinement of penicillopepsin at 1.8 Å resolution

1983 • 405 citations

PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors.

1986 • 400 citations

Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae

1983 • 337 citations

Genetics and Molecular Biology of Industrial Microorganisms

1989 • 319 citations

Developments in industrial microbiology

1983 • 310 citations

Heterologous Protein Secretion from Yeast

1985 • 299 citations

Evolution in the structure and function of aspartic proteases

1987 • 298 citations

The PEP4 Gene Encodes an Aspartyl Protease Implicated in the Posttranslational Regulation of Saccharomyces cerevisiae Vacuolar Hydrolases

1986 • 292 citations

Molecular and cellular basis of digestion

1986 • 288 citations

High Level Expression of Recombinant Genes in Aspergillus Oryzae

1988 • 259 citations

Expression, Glycosylation, and Secretion of an Aspergillus Glucoamylase by Saccharomyces cerevisiae

1985 • 248 citations

Purification of Calf Prochymosin (Prorennin) Synthesized in Escherichia Coli

1984 • 227 citations

Aspartic proteinases and their inhibitors

1985 • 218 citations

The Saccharomyces cerevisiae BAR1 gene encodes an exported protein with homology to pepsin.

1988 • 212 citations

The PEP4 gene encodes an aspartyl protease implicated in the posttranslational regulation of Saccharomyces cerevisiae vacuolar hydrolases.

1986 • 192 citations

Structure and refinement at 1.8 Å resolution of the aspartic proteinase from Rhizopus chinensis

1987 • 186 citations

Three-dimensional structure of the complex of the Rhizopus chinensis carboxyl proteinase and pepstatin at 2.5 .ANG. resolution

1982 • 185 citations

Improved Production of Chymosin in Aspergillus by Expression as a Glucoamylase-Chymosin Fusion

1990 • 175 citations

Synthesis of calf prochymosin (prorennin) in Escherichia coli.

1983 • 141 citations

A Novel Fungal Expression System: Secretion of Active Calf Chymosin from the Filamentous Fungus Trichoderma Reesei

1989 • 135 citations

A novel aspartyl protease allowing KEX2‐independent MFα propheromone processing in yeast

1990 • 135 citations

Expression of calf prochymosin in Saccharomyces cerevisiae

1984 • 115 citations

The three‐dimensional structure of recombinant bovine chymosin at 2.3 Å resolution

1990 • 115 citations

Structure of ethanol-inhibited porcine pepsin at 2-A resolution and binding of the methyl ester of phenylalanyl-diiodotyrosine to the enzyme.

1984 • 109 citations

Investigations on the Activation of Bovine Prochymosin

1979 • 99 citations

Cloning and sequencing of a gene for Mucor rennin, an aspartate protease fromMucor pusillus

1986 • 75 citations

Isolation and sequencing of a genomic clone encoding aspartic proteinase of Rhizopus niveus

1988 • 70 citations

Primary structure of Mucor miehei aspartyl protease: evidence for a zymogen intermediate

1986 • 66 citations

Effects of glycosylation on the secretion and enzyme activity of Mucor rennin, an aspartic proteinase of Mucor pusillus, produced by recombinant yeast.

1990 • 64 citations

Developments in Industrial Microbiology

1988 • 55 citations

Alteration of catalytic properties of chymosin by site-directed mutagenesis

1989 • 51 citations

Protein engineering of chymosin; modification of the optimum pH of enzyme catalysis

1990 • 47 citations

Complete Amino Acid Sequence of Scytalidium lignicolum Acid Protease B1

1984 • 45 citations

Secretion of Mucor rennin, a fungal aspartic protease of Mucor pusillus, by recombinant yeast cells

1987 • 45 citations

Expression of cloned calf prochymosin cDNA under control of the tryptophan promoter

1984 • 43 citations

Secretion by yeast of the zymogen form of Mucor rennin, an aspartic proteinase of Mucor pusillus, and its conversion to the mature form

1989 • 42 citations

The high resolution structure of endothiapepsin

1985 • 42 citations

Amino acid sequence of endothiapepsin

1987 • 38 citations

Purification and characterization of the two molecular forms of Aspergillus oryzae acid protease

1976 • 36 citations

The amino acid sequence of rhizopuspepsin, an aspartic proteinase from Rhizopus chinensis.

1987 • 35 citations

Studies on new acid proteases from Scytalidium lignicolum M-133. II. Purification and some enzymatic properties of acid protease A and B of Scytalidium lignicolum ATCC 24568.

1974 • 34 citations

Amino acid sequence of rhizopuspepsin isozyme pI 5.

1987 • 34 citations

Enzymatic properties and processing of bovine prochymosin synthesized in Escherichia coli

1985 • 32 citations

Isolation and Amino Acid Sequence of a Peptide Containing an Epoxide-Reactive Residue from the Thermolysin-Digest of Scytalidium lignicolum Acid Protease B1

1986 • 31 citations

Renaturation and activation of calf prochymosin produced in an insoluble form in Escherichia coli

1984 • 31 citations

High-level secretion of a Rhizoupus niveus aspartic proteinase in Saccharomyces cerevisiae.

1990 • 26 citations

Cloning and sequence analysis of cDNA for Irpex lacteus aspartic proteinase.

1989 • 25 citations

Production of chymosin in Escherichia coli cells and its enzymatic properties.

1987 • 24 citations

X-Ray Analysis and Circular Dichroism of the Acid Protease from Endothia Parasitica and Chymosin

1977 • 23 citations

Expression of active mucor miehei aspartic protease in Mucor circinelloides

1987 • 22 citations

Amino acid sequence of penicillopepsin. IV. Myxobacter AL-1 protease II and Staphylococcus aureus protease fragments and homology with pig pepsin and chymosin

1976 • 22 citations

Pepstatin insensitive acid proteinases

1985 • 21 citations

Protein chemical characterization of Mucor pusillus aspartic proteinase Amino acid sequence homology with the other aspartic proteinases, disulfide bond arrangement and site of carbohydrate attachment

1988 • 21 citations

Nucleotide sequence of the secretable acid protease gene PEP1 in the yeast Saccharomycopsis fibuligera.

1988 • 16 citations

Chemical Properties of the Polysaccharides Associated with Acid Protease of Aspergillus oryzae Grown on Solid Bran Media

1977 • 16 citations

A mutated bovine prochymosin zymogen can be activated without proteolytic processing at low pH.

1986 • 15 citations

Inhibition of Scytalidium lignicolum acid protease B by 1-diazo-3-phenyl-2-propanone.

1989 • 12 citations

Inactivation of Scytalidium lignicolum acid protease B with 1,2-epoxy-3-(4'-azido-2'-nitrophenoxy)propane.

1989 • 11 citations

Unusual zymogen‐processing properties of a mutated form of prochymosin

1988 • 10 citations

Prochymosin activation by non‐aspartic proteinases

1990 • 6 citations

Correct splicing of modified introns of a Rhizopus proteinase gene in Saccharomyces cerevisiae

1990 • 6 citations