Expression of active mucor miehei aspartic protease in Mucor circinelloides

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

1979 • 15,511 citations

Studies on transformation of Escherichia coli with plasmids

1983 • 11,179 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

1975 • 3,483 citations

Calcium-dependent bacteriophage DNA infection

1970 • 2,743 citations

SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN Escherichia coli : PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM

1969 • 1,847 citations

Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels

1977 • 1,607 citations

A RAPID ALKALINE EXTRACTION PROCEDURE FOR SCREENING RECOMBINANT DNA

1979 • 1,402 citations

Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli

1987 • 929 citations

Crossed Immunoelectrophoresis

1973 • 445 citations

Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans.

1985 • 279 citations

Transformation of Aspergillus nidulans by the orotidine-5′-phosphate decarboxylase gene of Neurospora crassa

1983 • 276 citations

High frequency transformation of Mucor with recombinant plasmid DNA

1984 • 143 citations

Transformation of the fungal maize pathogen Cochliobolus heterostrophus using the Aspergillus nidulans amdS gene

1985 • 136 citations

Transformation of Aspergillus niger using the argB gene of Aspergillus nidulans

1985 • 105 citations

Milk Clotting Enzyme from Microorganisms:Part I. Screening Test and the Identification of the Potent Fungus

1967 • 73 citations

Transformation of Phycomyces blakesleeanus to G-418 resistance by an autonomously replicating plasmid.

1986 • 71 citations

Heavily methylated amplified DNA in transformants of Neurospora crassa

1984 • 71 citations

Primary structure of Mucor miehei aspartyl protease: evidence for a zymogen intermediate

1986 • 66 citations

Enrichment method for the isolation of auxotrophic mutants of Mucor using the polyene antibiotic N-glycosyl-polifungin

1984 • 61 citations

Autonomous replication of plasmids in Mucor transformants

1986 • 60 citations

Transformation ofNeurospora crassa with circular and linear DNA and analysis of the fate of the transforming DNA

1985 • 58 citations

Milk Clotting Enzyme from Microorganisms

1967 • 56 citations

The isolation and partial characterization of an acid protease produced by Mucor miehei.

1970 • 41 citations

Transformation of Ustilago maydis by a plasmid containing yeast 2-micron DNA

1983 • 38 citations

Immunochemical, chromatographic, and milk-clotting activity measurements for quantification of milk-clotting enzymes in bovine rennets

1976 • 38 citations

On certain species of mucor with a key to all accepted species ; On the genera rhizomucor and parasitella

1978 • 38 citations

Structural and functional determinants of Mucor miehei protease III. Isolation and composition of the carbohydrate moiety

1974 • 27 citations

The reaction of partial identity in quantitative immunoelectrophoretic patterns

1972 • 22 citations

Commercial aspects of aspartic proteases

1985 • 7 citations

[Production and properties of the milk-clotting enzyme].

1983 • 7 citations

Studies on milk clotting enzyme from microorganisms, 1: Screening test and identification of a potent fungus for producing milk clotting enzyme and improvement of its enzymatic properties by using mutants of the fungus [cheeses]

1981 • 4 citations

Untitled

1981 • 2 citations

[Production of a concentrate of Mucor bacilliformis acid protease].

1982 • 2 citations