Effects of glycosylation on the secretion and enzyme activity of Mucor rennin, an aspartic proteinase of Mucor pusillus, produced by recombinant yeast.

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing

1980 • 3,476 citations

THE ESTIMATION OF PEPSIN, TRYPSIN, PAPAIN, AND CATHEPSIN WITH HEMOGLOBIN

1938 • 3,230 citations

Transformation of yeast by a replicating hybrid plasmid

1978 • 1,362 citations

KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene

1989 • 684 citations

The Release of Intact Oligosaccharides from Specific Glycoproteins by Endo-β-N-acetylglucosaminidase H

1974 • 499 citations

S. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiP

1989 • 421 citations

Glycosylation site binding protein, a component of oligosaccharyl transferase, is highly similar to three other 57 kd luminal proteins of the ER

1988 • 160 citations

Oligosaccharyl transferase: the central enzyme in the pathway of glycoprotein assembly

1987 • 160 citations

Homology among acid proteases: comparison of crystal structures at 3A resolution of acid proteases from Rhizopus chinensis and Endothia parasitica.

1977 • 119 citations

Cloning and sequencing of a gene for Mucor rennin, an aspartate protease fromMucor pusillus

1986 • 75 citations

The pH dependence of the hydrolysis of chromogenic substrates of the type, Lys-Pro-Xaa-Yaa-Phe-(NO2)Phe-Arg-Leu, by selected aspartic proteinases: evidence for specific interactions in subsites S3 and S2

1987 • 67 citations

Alteration of catalytic properties of chymosin by site-directed mutagenesis

1989 • 51 citations

Nucleotide sequence of the transcriptional initiation region of the yeastGAL7gene

1983 • 46 citations

Secretion of Mucor rennin, a fungal aspartic protease of Mucor pusillus, by recombinant yeast cells

1987 • 45 citations

Milk Clotting Enzyme from Microorganisms

1967 • 42 citations

Secretion by yeast of the zymogen form of Mucor rennin, an aspartic proteinase of Mucor pusillus, and its conversion to the mature form

1989 • 42 citations

Protein chemical characterization of Mucor pusillus aspartic proteinase Amino acid sequence homology with the other aspartic proteinases, disulfide bond arrangement and site of carbohydrate attachment

1988 • 21 citations

Role of N‐linked oligosaccharides attached to human renin expressed in COS cells

1988 • 20 citations