Probing the Cytoskeleton by Microinjection

Coated pits, coated vesicles, and receptor-mediated endocytosis

1979 • 2,073 citations

Intracellular Protein Degradation in Mammalian and Bacterial Cells

1974 • 1,775 citations

Microtubule Formation in vitro in Solutions Containing Low Calcium Concentrations

1972 • 1,201 citations

Intracellular Protein Degradation in Mammalian and Bacterial Cells: Part 2

1976 • 1,060 citations

Intermediate Filaments: A Chemically Heterogeneous, Developmentally Regulated Class of Proteins

1982 • 1,021 citations

Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line.

1982 • 923 citations

Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubules

1974 • 814 citations

A 130K protein from chicken gizzard: Its localization at the termini of microfilament bundles in cultured chicken cells

1979 • 794 citations

Molecules and Cell Movement

1977 • 767 citations

Structural interaction of cytoskeletal components

1981 • 766 citations

Actin polymerization and its regulation by proteins from nonmuscle cells.

1982 • 739 citations

Head to tail polymerization of actin

1976 • 692 citations

Cross-linker system between neurofilaments, microtubules and membranous organelles in frog axons revealed by the quick-freeze, deep-etching method

1982 • 672 citations

Clathrin: a unique protein associated with intracellular transfer of membrane by coated vesicles.

1976 • 663 citations

Actin Is the Naturally Occurring Inhibitor of Deoxyribonuclease I

1974 • 637 citations

Exchange of actin subunits at the leading edge of living fibroblasts: possible role of treadmilling.

1985 • 637 citations

α-Actinin: Immunofluorescent localization of a muscle structural protein in nonmuscle cells

1975 • 599 citations

The Cell Nucleus

1966 • 522 citations

Actin-binding proteins—regulators of cell architecture and motility

1982 • 468 citations

Actin, alpha-actinin, and tropomyosin interaction in the structural organization of actin filaments in nonmuscle cells.

1976 • 454 citations

The effect of myosin antibody on the division of starfish blastomeres

1977 • 445 citations

Villin is a major protein of the microvillus cystoskeleton which binds both G and F actin in a calcium-dependent manner

1980 • 444 citations

Fluorescence staining of the actin cytoskeleton in living cells with 7-nitrobenz-2-oxa-1,3-diazole-phallacidin.

1980 • 441 citations

Functional Aspects of Satellite DNA and Heterochromatin

1979 • 425 citations

Differentiation-related Patterns of Expression of Proteins of Intermediate-size Filaments in Tissues and Cultured Cells

1982 • 419 citations

Immunohistochemical localization of a macrophage-specific antigen in developing mouse retina: phagocytosis of dying neurons and differentiation of microglial cells to form a regular array in the plexiform layers.

1983 • 412 citations

Microinjection and localization of a 130K protein in living fibroblasts: a relationship to actin and fibronectin

1980 • 406 citations

10-nm filaments are induced to collapse in living cells microinjected with monoclonal and polyclonal antibodies against tubulin.

1984 • 388 citations

Co-existence of vinculin and a vinculin-like protein of higher molecular weight in smooth muscle.

1982 • 343 citations

Molecular biology of the cytoskeleton

1984 • 296 citations

Nonerythrocyte spectrins: actin-membrane attachment proteins occurring in many cell types

1982 • 295 citations

Microinjection of actin-binding proteins and actin antibodies demonstrates involvement of nuclear actin in transcription of lampbrush chromosomes

1984 • 291 citations

Involvement of vesicle coat material in casein secretion and surface regeneration.

1976 • 256 citations

Microtubule Assembly and Nucleation

1978 • 253 citations

Radioimmunoassay for tubulin: a quantitative comparison of the tubulin content of different established tissue culture cells and tissues

1978 • 253 citations

Bioenergetics and Kinetics of Microtubule and Actin Filament Assembly–Disassembly

1982 • 242 citations

Is actin a transcription initiation factor for RNA polymerase B?

1984 • 234 citations

Interaction of alpha-actinin and vinculin with actin: opposite effects on filament network formation.

1981 • 230 citations

Erythroid spectrin, brain fodrin, and intestinal brush border proteins (TW-260/240) are related molecules containing a common calmodulin-binding subunit bound to a variant cell type-specific subunit.

1982 • 228 citations

Tubulin domains probed by limited proteolysis and subunit-specific antibodies

1985 • 224 citations

THE CONTRACTILE BASIS OF AMOEBOID MOVEMENT

1973 • 220 citations

Direct demonstration of the presence of two immunologically distinct intermediate-sized filament systems in the same cell by double immunofluorescence microscopy

1980 • 211 citations

Mobility of microinjected rhodamine actin within living chicken gizzard cells determined by fluorescence photobleaching recovery

1982 • 206 citations

A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. I. Biochemical characterization, effects on microtubule polymerization in vitro, and microtubule polymerization and organization in vivo.

1983 • 197 citations

Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth

1977 • 194 citations

Studies on the in vivo sensitivity of spindle microtubules to calcium ions and evidence for a vesicular calcium-sequestering system.

1981 • 193 citations

Actin-binding proteins

1982 • 192 citations

Fluorescently labelled molecules as probes of the structure and function of living cells

1980 • 189 citations

In Vitro Assembly of Cytoplasmic Microtubules

1980 • 189 citations

Cell and Muscle Motility

1982 • 185 citations

Two general classes of cytoplasmic actin filaments in tissue culture cells: The role of tropomyosin

1976 • 176 citations

Tropomyosin is decreased in transformed cells.

1981 • 171 citations

Disruption of the in vivo distribution of the intermediate filaments in fibroblasts through the microinjection of a specific monoclonal antibody

1981 • 170 citations

Intermediate filaments in 3T3 cells collapse after intracellular injection of a monoclonal anti-intermediate filament antibody

1981 • 169 citations

Molecular cytochemistry: incorporation of fluorescently labeled actin into living cells.

1978 • 149 citations

Transport of the membrane glycoprotein of vesicular stomatitis virus to the cell surface in two stages by clathrin-coated vesicles.

1980 • 146 citations

Reorganization of actin filament bundles in living fibroblasts.

1984 • 146 citations

Changing patterns of actin localization during cell division.

1975 • 142 citations

Differences in the stress fibers between fibroblasts and epithelial cells.

1983 • 140 citations

An actin filament matrix in hand-isolated nuclei of X. laevis oocytes

1979 • 139 citations

Morphology, behavior, and interaction of cultured epithelial cells after the antibody-induced disruption of keratin filament organization.

1983 • 136 citations

Actin filaments elongate from their membrane-associated ends

1981 • 135 citations

Intranuclear actin bundles induced by dimethyl sulfoxide in interphase nucleus of Dictyostelium.

1978 • 134 citations

Contractile basis of ameboid movement. VII. Aequorin luminescence during ameboid movement, endocytosis, and capping.

1980 • 133 citations

A major soluble acidic protein located in nuclei of diverse vertebrate species

1980 • 131 citations

Elevation of tubulin levels by microinjection suppresses new tubulin synthesis

1983 • 128 citations

Phalloidin-induced hyperplasia of actin filaments in rat hepatocytes.

1975 • 127 citations

Brush-border calmodulin. A major component of the isolated microvillus core.

1980 • 127 citations

Reversible translocation of cytoplasmic actin into the nucleus caused by dimethyl sulfoxide.

1980 • 127 citations

Immunoprecipitation of nonerythrocyte spectrin within live cells following microinjection of specific antibodies: relation to cytoskeletal structures.

1984 • 125 citations

A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. II. Effects on cell movement, organization of microtubules, and intermediate filaments, and arrangement of Golgi elements.

1983 • 125 citations

Evidence that myosin does not contribute to force production in chromosome movement.

1982 • 125 citations

High-affinity interaction of vinculin with actin filaments in vitro

1982 • 125 citations

Association of microtubules and intermediate filaments in chicken gizzard cells as detected by double immunofluorescence.

1980 • 124 citations

Intranuclear injection of anti-actin antibodies into Xenopus oocytes blocks chromosome condensation

1979 • 122 citations

Degradation of proteins microinjected into IMR-90 human diploid fibroblasts.

1981 • 121 citations

Distribution of fluorescently labeled actin in living sea urchin eggs during early development.

1979 • 121 citations

Nuclear actin bundles in Amoeba, dictyostelium and human HeLa cells induced by dimethyl sulfoxide

1979 • 116 citations

Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments.

1985 • 114 citations

Microinjection of fluorescently labeled α-actinin into living fibroblasts

1979 • 113 citations

Cross-reactivity between Thy-1 and a component of intermediate filaments demonstrated using a monoclonal antibody

1981 • 112 citations

In vivo distribution and turnover of fluorescently labeled actin microinjected into human fibroblasts.

1979 • 110 citations

Analysis of myofibrillar structure and assembly using fluorescently labeled contractile proteins.

1984 • 108 citations

Assembled and unassembled pools of clathrin: a quantitative study using an enzyme immunoassay.

1985 • 107 citations

Inhibitors of dynein activity block intracellular transport in erythrophores

1982 • 105 citations

Microinjection of Fluorescently Labeled Proteins into Living Cells with Emphasis on Cytoskeletal Proteins

1982 • 102 citations

Contractile basis of ameboid movement. VII. The distribution of fluorescently labeled actin in living amebas.

1980 • 97 citations

Coiling of intermediate filaments induced by microinjection of a vimentin-specific antibody does not interfere with locomotion and mitosis.

1981 • 96 citations

Partial reconstruction of the microvillus core bundle: characterization of villin as a Ca(++)-dependent, actin-bundling/depolymerizing protein

1982 • 96 citations

Probing microtubule-dependent intracellular motility with nanometre particle video ultramicroscopy (nanovid ultramicroscopy).

1985 • 96 citations

Direct visualization of fluorescein-labeled microtubules in vitro and in microinjected fibroblasts.

1981 • 96 citations

Evidence for the autophagy of microinjected proteins in HeLA cells.

1977 • 95 citations

Involvement of contractile proteins in the changes in consistency of oocyte nucleoplasm of the newt Pleurodeles waltlii.

1981 • 94 citations

Rapid transport of foreign particles microinjected into crab axons

1983 • 89 citations

De novo synthesis and specific assembly of keratin filaments in nonepithelial cells after microinjection of mRNA for epidermal keratin

1983 • 89 citations

Meta-vinculin—a vinculin-related protein with solubility properties of a membrane protein

1982 • 88 citations

Distribution of fluorescently labeled actin and tropomyosin after microinjection in living tissue culture cells as observed with TV image intensification

1980 • 84 citations

Newly synthesized G protein of vesicular stomatitis virus is not transported to the cell surface during mitosis.

1983 • 84 citations

Nuclear proteins. II. Similarity of nonhistone proteins in nuclear sap and chromatin, and essential absence of contractile proteins from mouse liver nuclei.

1976 • 83 citations

Slime mould actin: Homology to vertebrate actin and presence in the nucleus*1

1974 • 82 citations

Identical distribution of fluorescently labeled brain and muscle actins in living cardiac fibroblasts and myocytes.

1985 • 78 citations

Lack of tropomyosin correlates with the absence of stress fibers in transformed rat kidney cells

1982 • 78 citations

Preparation and characterization of a new molecular cytochemical probe: 5-iodoacetamidofluorescein-labeled actin.

1980 • 78 citations

Kinetic analysis of actin assembly suggests that tropomyosin inhibits spontaneous fragmentation of actin filaments

1982 • 77 citations

Chapter 15 The Cyclic Tyrosination/Detyrosination of Alpha Tubulin

1982 • 77 citations

Hormones and cell regulation

1977 • 76 citations

Villin associates with specific microfilamentous structures as seen by immunofluorescence microscopy on tissue sections and cells microinjected with villin

1981 • 75 citations

Coated vesicles

1980 • 75 citations

Evidence that coated vesicles transport acetylcholine receptors to the surface membrane of chick myotubes.

1984 • 74 citations

Molecular Basis of Motility

1976 • 72 citations

Actin filament disassembly in blood plasma

1980 • 71 citations

Microheterogeneity of avian and mammalian vinculin

1982 • 67 citations

Degradation of proteins microinjected into cultured mammalian cells

1979 • 66 citations

Immuno-electron microscopical identification of the two types of intermediate filaments in established epithelial cells

1981 • 66 citations

Hormones and cell regulation

1991 • 65 citations

Dynamics of nuclear actin bundle induction by dimethyl sulfoxide and factors affecting its development.

1980 • 64 citations

Actin filament capping protein from bovine brain.

1982 • 63 citations

Epithelial Tonofilaments: Investigating Their Form and Function Using Monoclonal Antibodies

1982 • 62 citations

An actin depolymerizing protein from pig plasma

1981 • 60 citations

Microinjection of anticlathrin antibodies into fibroblasts does not interfere with the receptor-mediated endocytosis of α2-macroglobulin

1981 • 60 citations

The presence of actin in nuclei: a critical appraisal

1977 • 59 citations

Distribution of flourescently labeled α-actinin in living and fixed fibroblasts

1980 • 56 citations

Coordinated synthesis and degradation of actin and myosin in a variety of myogenic and non-myogenic cells

1976 • 56 citations

Cell and muscle motility

1985 • 56 citations

Disruption of microfilament organization after injection of F-actin capping proteins into living tissue culture cells

1983 • 55 citations

Subcellular distribution of rhodamine-actin microinjected into living fibroblastic cells.

1983 • 55 citations

Demonstration of three distinct calcium-binding sites in villin, a modulator of actin assembly.

1983 • 55 citations

Pattern and time course of rhodamine-actin incorporation in cardiac myocytes.

1983 • 54 citations

Microinjection of fluorescent tubulin into dividing sea urchin cells.

1983 • 54 citations

Microinjected fluorescent polystyrene beads exhibit saltatory motion in tissue culture cells.

1984 • 54 citations

Vimentin and keratin intermediate filament systems in cultured PtK2 epithelial cells are interrelated.

1982 • 53 citations

Acumentin, a protein in macrophages which caps the ‘pointed’ end of actin filaments

1982 • 52 citations

Interaction of fluorescently-labeled contractile proteins with the cytoskeleton in cell models.

1984 • 52 citations

Effects of phalloidin microinjection and localization of fluorescein-labeled phalloidin in living sand dollar eggs

1982 • 50 citations

Dynamic interactions of fluorescently labeled microtubule-associated proteins in living cells.

1984 • 49 citations

Actin rearrangement in living cells revealed by microinjection of a fluorescent phalloidin derivative.

1981 • 47 citations

Actin co-purifies with RNA polymerase II

1979 • 46 citations

The influence of microinjected phalloidin on locomotion, protoplasmic streaming and cytoplasmic organization in Amoeba proteus and Physarum polycephalum.

1978 • 45 citations

Dynamics of the cytoskeleton in Amoeba proteus

1983 • 45 citations

Differential response of three types of actin filament bundles to depletion of cellular ATP levels.

1983 • 43 citations

Vanadate inhibits saltatory organelle movement in a permeabilized cell model

1982 • 41 citations

Tropomyosin as a modulator of microfilaments

1984 • 40 citations

Effect of microinjected N-ethylmaleimide-modified heavy meromyosin on cell division in amphibian eggs.

1980 • 38 citations

Effects of the actin-binding protein DNAase I on cytoplasmic streaming and ultrastructure of Amoeba proteus

1979 • 38 citations

In Vivo Disruption of the Cytokeratin Cytoskeleton in Cultured Epithelial Cells by Microinjection of Antikeratin: Evidence for the Presence of an Intermediate-filament-organizing Center

1982 • 37 citations

Human plasma actin‐depolymerizing factor

1985 • 37 citations

Equilibrium constant for binding of an actin filament capping protein to the barbed end of actin filaments

1985 • 35 citations

Distribution of fluorescently labeled tubulin injected into sand dollar eggs from fertilization through cleavage.

1985 • 34 citations

Organization and spatial arrangement of fluorescein-labeled native actin microinjected into normal locomoting and experimentally influenced Amoeba proteus

1980 • 34 citations

Pinocytosis and locomotion in amoebae

1979 • 34 citations

Phalloidin associates with microfilaments after microinjection into tissue culture cells.

1980 • 33 citations

Ca2+-dependent actin-binding phosphoprotein in Physarum polycephalum. I. Ca2+/actin-dependent inhibition of its phosphorylation.

1983 • 31 citations

Evidence for actin transformation during the contraction-relaxation cycle of cytoplasmic actomyosin: Cycle blockade by Phalloidin injection

1979 • 31 citations

T Antigens of SV40-transformed Cells

1980 • 30 citations

Autoregulatory Control of the Expression of -and -Tubulins: Implications for Microtubule Assembly

1982 • 30 citations

Dissolution of cytoplasmic actin bundles and the induction of nuclear actin bundles by dimethyl sulfoxide

1980 • 27 citations

Receptor-mediated Endocytosis by Clathrin-coated Vesicles: Evidence for a Dynamic Pathway

1982 • 26 citations

Visualization of actin polymerization and depolymerization cycles during polyamine-induced cytokinesis in living Amoeba proteus

1981 • 26 citations

Cytoskeletal organization in locomoting cells of the V2 rabbit carcinoma*1

1983 • 25 citations

Intracellular and Extracellular Components Involved in the Formation of Ventral Surfaces of Fibroblasts

1982 • 25 citations

Patterns of microfilament organization in animal cells

1983 • 24 citations

Pinocytosis and locomotion of amoebae

1980 • 22 citations

Ionic mechanisms in secretagogue-induced morphological changes in rat parotid gland.

1983 • 21 citations

Function of the microfilament system in living cell fragments of Physarum polycephalum as revealed by microinjection of fluorescent analogs

1985 • 20 citations

Modification of Vimentin Polypeptides during Mitosis

1982 • 20 citations

Dynamics of the cytoskeleton inAmoeba proteus: II. Influence of different agents on the spatial organization of microinjected fluorescein-labeled actin

1984 • 20 citations

Ciliary but not saltatory movements are inhibited by vanadate microinjected into living cultured cells

1983 • 19 citations

Disruption of microtubules in living cells and cell models by high affinity antibodies to beta-tubulin.

1985 • 18 citations

Gene expression in brain

1987 • 16 citations

Organization and function of structural elements in focal contacts of tissue culture cells

1983 • 14 citations

A preliminary communication on certain specific reactions exhibited by hay fever cases

1913 • 13 citations

Microinjection of Anticlathrin Antibodies into Cultured Fibroblasts: Clathrin-coated Structures in Receptor-mediated Endocytosis and in Exocytosis

1982 • 12 citations

Cell β-Actinin, an Accelerator of Actin Polymerization, Isolated from Rat Kidney Cytosol<xref ref-type="fn" rid="fn1"><sup>1</sup></xref>

1981 • 5 citations

Molekular- und Zellbiologie : Aktuelle Themen

1985 • 4 citations

Analyse des Mikrofilamentsystems tierischer Zellen durch Mikroinjektion

1984 • 3 citations

The microtubular cytoskeleton in developing pollen of Gasteria

1985 • 1 citations

Deleted Work

1955 • 0 citations