The yeast alpha 2 protein can repress transcription by RNA polymerases I and II but not III.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Experiments in molecular genetics

1972 • 28,260 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Genomic sequencing.

1984 • 8,343 citations

Personal communication

1989 • 2,984 citations

Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase

1982 • 1,539 citations

Transcriptional Control Signals of a Eukaryotic Protein-Coding Gene

1982 • 1,406 citations

Position effect at S. cerevisiae telomeres: Reversible repression of Pol II transcription

1990 • 1,343 citations

A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3′ border of the region

1980 • 734 citations

Isolation of coactivators associated with the TATA-binding protein that mediate transcriptional activation

1991 • 711 citations

Four Genes Responsible for a Position Effect on Expression From HML and HMR in Saccharomyces cerevisiae

1987 • 709 citations

TRANSCRIPTION BY RNA POLYMERASE III

1988 • 704 citations

Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiae

1991 • 700 citations

Ssn6-Tup1 is a general repressor of transcription in yeast

1992 • 680 citations

Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases

1985 • 670 citations

Transcription from a TATA-less promoter requires a multisubunit TFIID complex.

1991 • 618 citations

Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae.

1981 • 542 citations

Characterization of a “silencer” in yeast: A DNA sequence with properties opposite to those of a transcriptional enhancer

1985 • 531 citations

Eukaryotic RNA Polymerase

1985 • 500 citations

[76] β-d-Fructofuranoside fructohydrolase from yeast

1975 • 455 citations

Spliceosomal RNA U6 is remarkably conserved from yeast to mammals

1988 • 428 citations

The TATA-binding protein and associated factors are integral components of the RNA polymerase I transcription factor, SL1

1992 • 427 citations

Human general transcription factor IIH phosphorylates the C-terminal domain of RNA polymerase II

1992 • 407 citations

The TATA-binding protein is required for transcription by all three nuclear RNA polymerases in yeast cells

1992 • 392 citations

Coactivators for a proline-rich activator purified from the multisubunit human TFIID complex.

1991 • 381 citations

A repressor (MATα2 product) and its operator control expression of a set of cell type specific genes in yeast

1985 • 368 citations

Prokaryotic and eukaryotic RNA polymerases have homologous core subunits.

1987 • 355 citations

The yeast cell-type-specific repressor α2 acts cooperatively with a non-cell-type-specific protein

1988 • 348 citations

The nonphosphorylated form of RNA polymerase II preferentially associates with the preinitiation complex.

1991 • 319 citations

Saccharomyces telomeres assume a non-nucleosomal chromatin structure.

1992 • 275 citations

The regulation of yeast mating-type chromatin structure by SIR: An action at a distance affecting both transcription and transposition

1982 • 257 citations

Transcription and regulatory signals at the mating type locus in yeast

1984 • 256 citations

Transcription factor IIIB generates extended DNA interactions in RNA polymerase III transcription complexes on tRNA genes.

1989 • 248 citations

The major promoter element of rRNA transcription in yeast lies 2 kb upstream

1984 • 246 citations

A protein involved in minichromosome maintenance in yeast binds a transcriptional enhancer conserved in eukaryotes.

1989 • 239 citations

MATα1 protein, a yeast transcription activator, binds synergistically with a second protein to a set of cell-type-specific genes

1987 • 236 citations

The transition of RNA polymerase II from initiation to elongation is associated with phosphorylation of the carboxyl-terminal domain of subunit IIa

1989 • 236 citations

A position effect in the control of transcription at yeast mating type loci

1981 • 233 citations

Variants of the TATA-binding protein can distinguish subsets of RNA polymerase I, II, and III promoters

1992 • 228 citations

A SUPPRESSOR OF snf1 MUTATIONS CAUSES CONSTITUTIVE HIGH-LEVEL INVERTASE SYNTHESIS IN YEAST

1984 • 228 citations

Telomere-proximal DNA in Saccharomyces cerevisiae is refractory to methyltransferase activity in vivo.

1992 • 227 citations

A common octamer motif binding protein is involved in the transcription of U6 snRNA by RNA polymerase III and U2 snRNA by RNA polymerase II

1987 • 224 citations

A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae

1988 • 221 citations

Upstream regulatory elements are necessary and sufficient for transcription of a U6 RNA gene by RNA polymerase III.

1988 • 215 citations

Regulation of transcription in expressed and unexpressed mating type cassettes of yeast

1981 • 210 citations

Nucleosomes are positioned with base pair precision adjacent to the alpha 2 operator in Saccharomyces cerevisiae.

1991 • 203 citations

A yeast operator overlaps an upstream activation site

1987 • 200 citations

Molecular analysis of SSN6, a gene functionally related to the SNF1 protein kinase of Saccharomyces cerevisiae.

1987 • 192 citations

Active genes in budding yeast display enhanced in vivo accessibility to foreign DNA methylases: a novel in vivo probe for chromatin structure of yeast.

1992 • 188 citations

Internal control regions for transcription of eukaryotic tRNA genes.

1981 • 186 citations

Participation of the TATA Factor in Transcription of the Yeast U6 Gene by RNA Polymerase C

1991 • 185 citations

RPC40, a unique gene for a subunit shared between yeast RNA polymerases A and C

1987 • 184 citations

A yeast TFIIB-related factor involved in RNA polymerase III transcription.

1992 • 181 citations

Multiple roles for U6 snRNA in the splicing pathway.

1990 • 179 citations

Phosphorylation of RNA polymerase IIA occurs subsequent to interaction with the promoter and before the initiation of transcription.

1990 • 178 citations

Subunits shared by eukaryotic nuclear RNA polymerases.

1990 • 175 citations

Synthesis of large rRNAs by RNA polymerase II in mutants of Saccharomyces cerevisiae defective in RNA polymerase I.

1991 • 174 citations

A Drosophila rRNA gene located in euchromatin is active in transcription and nucleolus formation.

1988 • 171 citations

In vitro mutation analysis of the mating-type locus in yeast

1981 • 170 citations

Changing the RNA polymerase specificity of U snRNA gene promoters

1988 • 170 citations

rRNA synthesis in the nucleolus

1990 • 164 citations

Yeast alpha 2 repressor positions nucleosomes in TRP1/ARS1 chromatin.

1990 • 161 citations

Two components of Saccharomyces cerevisiae transcription factor IIIB (TFIIIB) are stereospecifically located upstream of a tRNA gene and interact with the second-largest subunit of TFIIIC.

1991 • 160 citations

Stable nucleosome positioning and complete repression by the yeast alpha 2 repressor are disrupted by amino-terminal mutations in histone H4.

1992 • 160 citations

PCF4 encodes an RNA polymerase III transcription factor with homology to TFIIB

1992 • 157 citations

Upstream elements required for efficient transcription of a human U6 RNA gene resemble those of U1 and U2 genes even though a different polymerase is used.

1988 • 157 citations

A suppressor of TBP mutations encodes an RNA polymerase III transcription factor with homology to TFIIB

1992 • 155 citations

An RNA polymerase I enhancer in Saccharomyces cerevisiae.

1986 • 151 citations

Transcription of a yeast U6 snRNA gene requires a polymerase III promoter element in a novel position.

1990 • 150 citations

The cloned RNA polymerase II transcription factor IID selects RNA polymerase III to transcribe the human U6 gene in vitro.

1991 • 143 citations

A MUTATION THAT PERMITS THE EXPRESSION OF NORMALLY SILENT COPIES OF MATING-TYPE INFORMATION IN SACCHAROMYCES CEREVISIAE

1979 • 138 citations

A role for the TATA-box-binding protein component of the transcription factor IID complex as a general RNA polymerase III transcription factor.

1992 • 129 citations

TFIID is required for in vitro transcription of the human U6 gene by RNA polymerase III.

1991 • 128 citations

The interaction of RNA polymerase II with the adenovirus-2 major late promoter is precluded by phosphorylation of the C-terminal domain of subunit IIa.

1992 • 128 citations

Yeast repressor alpha 2 binds to its operator cooperatively with yeast protein Mcm1.

1989 • 125 citations

RPA190, the gene coding for the largest subunit of yeast RNA polymerase A.

1988 • 124 citations

The N-terminal TPR region is the functional domain of SSN6, a nuclear phosphoprotein of Saccharomyces cerevisiae.

1990 • 124 citations

RPC19, the gene for a subunit common to yeast RNA polymerases A (I) and C (III)

1991 • 118 citations

Mutations at the yeast SUP4 tRNATyr locus: DNA sequence changes in mutants lacking suppressor activity

1980 • 115 citations

Identification, purification, and cloning of a polypeptide (PRTF/GRM) that binds to mating-specific promoter elements in yeast.

1990 • 112 citations

Effects of altered 5'-flanking sequences on the in vivo expression of a Saccharomyces cerevisiae tRNATyr gene.

1984 • 104 citations

U6 snRNA genes ofArabidopsisare transcribed by RNA polymerase III but contain the same two upstream promoter elements as RNA polymerase ll-transcribed U-snRNA genes

1990 • 100 citations

The HML mating-type cassette of Saccharomyces cerevisiae is regulated by two separate but functionally equivalent silencers.

1989 • 99 citations

The c-myc gene encodes superimposed RNA polymerase II and III promoters

1987 • 99 citations

A position effect on the expression of a tRNA gene mediated by the SIR genes in Saccharomyces cerevisiae.

1986 • 96 citations

Negative regulation of STE6 gene expression by the alpha 2 product of Saccharomyces cerevisiae.

1984 • 93 citations

An approach for isolation of mutants defective in 35S ribosomal RNA synthesis in Saccharomyces cerevisiae.

1991 • 88 citations

A transcriptionally active tRNA gene interferes with nucleosome positioning in vivo.

1992 • 88 citations

Suppressor analysis of temperature-sensitive mutations of the largest subunit of RNA polymerase I in Saccharomyces cerevisiae: a suppressor gene encodes the second-largest subunit of RNA polymerase I.

1991 • 85 citations

AAR1/TUP1 Protein, with a Structure Similar to That of the β Subunit of G Proteins, Is Required for a1-α2 and α2 Repression in Cell Type Control of Saccharomyces cerevisiae

1991 • 82 citations

RNA polymerases B and C are more closely related to each other than to RNA polymerase A.

1988 • 81 citations

Isolation of Saccharomyces cerevisiae mutants constitutive for invertase synthesis

1986 • 80 citations

Linker scanning of the yeast RNA polymerase I promoter

1989 • 78 citations

The RET1 gene of yeast encodes the second-largest subunit of RNA polymerase III. Structural analysis of the wild-type and ret1-1 mutant alleles.

1991 • 77 citations

PLEIOTROPIC MUTATIONS AT THE TUP1 LOCUS THAT AFFECT THE EXPRESSION OF MATING-TYPE-DEPENDENT FUNCTIONS IN SACCHAROMYCES CEREVISIAE

1980 • 76 citations

5 Eukaryotic RNA Polymerases

1982 • 75 citations

Two additional common subunits, ABC10 alpha and ABC10 beta, are shared by yeast RNA polymerases.

1991 • 72 citations

Sequences upstream of the STE6 gene required for its expression and regulation by the mating type locus in Saccharomyces cerevisiae.

1986 • 70 citations

Alteration of hsp82 gene expression by the gypsy transposon and suppressor genes in Drosophila melanogaster.

1989 • 69 citations

Genomic footprinting of a yeast tRNA gene reveals stable complexes over the 5'-flanking region.

1989 • 67 citations

RNA polymerase III catalysed transcription can be regulated in Saccharomyces cerevisiae by the bacterial tetracycline repressor-operator system.

1992 • 64 citations

Organization and Expression of tRNA Genes in Saccharomyces cerevisiae

1982 • 64 citations

Transcription By RNA Polymerase III

1988 • 58 citations

In vitrodefinition of the yeast RNA polymerase I promoter

1992 • 53 citations

Unusual enhancer function in yeast rRNA transcription.

1989 • 51 citations

SWITCHING OF A MATING-TYPE a MUTANT ALLELE IN BUDDING YEAST SACCHAROMYCES CEREVISIAE

1979 • 48 citations

Comparison of tRNA gene transcription complexes formed in vitro and in nuclei.

1987 • 37 citations

Identification of a protein factor binding to the 5′-flanking region of a tRNA gene and being involved in modulation of tRNA gene transcriptionin vivoinSaccharomyces cerevisiae

1988 • 36 citations

Genomic Footprinting of a Yeast tRNA Gene Reveals Stable Complexes over the 5′-Flanking Region

1989 • 28 citations

Deletion mapping of the yeast pol I promoter

1985 • 28 citations

Unusual Enhancer Function in Yeast rRNA Transcription

1989 • 24 citations

Regulated Expression of a Mammalian Nonsense Suppressor tRNA Gene In Vivo and In Vitro Using the lac Operator/Repressor System

1992 • 21 citations

A Transcriptionally Active tRNA Gene Interferes with Nucleosome Positioning In Vivo

1992 • 19 citations

Yeast α2 Repressor Positions Nucleosomes in TRP1/ARS1 Chromatin

1990 • 16 citations

Comparison of tRNA Gene Transcription Complexes Formed In Vitro and in Nuclei

1987 • 16 citations

Transcriptional enhancement by upstream activators is brought about by different molecular mechanisms for class I and II RNA polymerase genes.

1989 • 12 citations

The yeast SFL2 gene may be necessary for mating-type control

1992 • 8 citations

Regulated expression of a mammalian nonsense suppressor tRNA gene in vivo and in vitro using the lac operator/repressor system.

1992 • 8 citations

The yeast rRNA gene enhancer does not function by recycling RNA polymerase I and cannot act as a UAS

1991 • 6 citations

Activity of promoter mutants of the yeast ribosomal RNA gene with and without the enhancer

1991 • 5 citations