Evidence that the lack of high catalytic activity of thiolsubtilisin towards specific substrates may be due to an inappropriately located proton-distribution system. Demonstration of highly nucleophilic character of the thiol group of thiolsubtilisin in the catalytically relevant ionization state of the active centre by use of a two-protonic-state reactivity probe

Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2′-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols

1973 • 163 citations

Neutron diffraction identifies His 57 as the catalytic base in trypsin

1980 • 139 citations

The Reactivity of Thiol-subtilisin, an Enzyme Containing a Synthetic Functional Group^*

1967 • 120 citations

Re-examination of the charge relay system in subtilisin comparison with other serine proteases.

1977 • 119 citations

Specific covalent modification of thiols: Applications in the study of enzymes and other biomolecules

1979 • 118 citations

Convergence of active center geometries

1977 • 89 citations

Properties of Thiol-Subtilisin

1968 • 80 citations

The equilibrium assumption is valid for the kinetic treatment of most time-dependent protein-modification reactions

1979 • 66 citations

Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe

1976 • 60 citations

Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system

1978 • 57 citations

The importance of the conformation of the tetrahedral intermediate for the α‐chymotrypsin‐catalyzed hydrolysis of peptide substrates

1975 • 47 citations

Thioltrypsin

1977 • 45 citations

Mechanism of the reaction of papain with substrate-derived diazomethyl ketones. Implications for the difference in site specificity of halomethyl ketones for serine proteinases and cysteine proteinases and for stereoelectronic requirements in the papain catalytic mechanism

1978 • 41 citations

Comparison of the kinetic specificity of subtilisin and thiolsubtilisin toward n-alkyl p-nitrophenyl esters

1979 • 35 citations

Mechanism of thiol protease catalysis: detection and stabilization of a tetrahedral intermediate in papain catalysis

1979 • 35 citations

Cryoenzymology of papain: reaction mechanism with an ester substrate

1978 • 34 citations

Chromatography and activity of thiol-subtilisin

1969 • 31 citations

Negatively Charged Reactants as Probes in the Study of the Essential Mercaptide‐Imidazolium Ion‐Pair of Thiolenzymes

1977 • 31 citations

On the Reactivity of the Thiol Group of Thiolsubtilisin

1973 • 30 citations

Mechanism of action of papain with a specific anilide substrate

1979 • 30 citations

Evidence for a two-state transition in papain that may have no close analogue in ficin. Differences in the disposition of cationic sites and hydrophobic binding areas in the active centres of papain and ficin

1980 • 24 citations

Transformation of L-Serine Peptides to L-Cysteine Peptides¹

1965 • 23 citations

Evidence that binding to the S2-subsite of papain may be coupled with catalytically relevant structural change involving the cysteine-25–histidine-159 diad. Kinetics of the reaction of papain with a two-protonic-state reactivity probe containing a hydrophobic side chain

1979 • 22 citations

Evidence that the active centre of chymopapain A is different from the active centres of some other cysteine proteinases and that the Brønsted coefficient (βnuc.) for the reactions of thiolate anions with 2,2′-dipyridyl disulphide may be decreased by reagent protonation

1980 • 13 citations

Evidence for association-activation effects in reactions of papain from studies on its reactivity towards isomeric two-protonic-state reactivity probes

1979 • 10 citations

Conformation of the active site of thiolsubtilisin: reaction with specific chloromethyl ketones and arylacryloylimidazoles

1979 • 10 citations

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