Mechanism of thiol protease catalysis: detection and stabilization of a tetrahedral intermediate in papain catalysis

The Determination of the Concentration of Hydrolytic Enzyme Solutions: α-Chymotrypsin, Trypsin, Papain, Elastase, Subtilisin, and Acetylcholinesterase¹

1966 • 386 citations

Subtilisin. Stereochemical mechanism involving transition-state stabilization

1972 • 376 citations

Binding of chloromethyl ketone substrate analogs to crystalline papain

1976 • 352 citations

Simple alkanethiol groups for temporary blocking of sulfhydryl groups of enzymes

1975 • 311 citations

Kinetics of Papain-Catalyzed Hydrolysis of α-N-Benzoyl-L-arginine Ethyl Ester and α-N-Benzoyl-L-argininamide¹

1965 • 204 citations

Oxygen Exchange as Evidence for the Existence of an Intermediate in Ester Hydrolysis

1951 • 190 citations

Chemical modification of papain

1968 • 189 citations

Aldehydes as Inhibitors of Papain

1972 • 169 citations

General Base and Nucleophilic Catalysis of Ester Hydrolysis and Related Reactions

1967 • 169 citations

The Purification of Papain by Affinity Chromatography

1970 • 160 citations

What limits the rate of an enzyme-catalyzed reaction

1975 • 148 citations

The Preparation and Properties of trans-Cinnamoyl-Papain¹

1966 • 119 citations

Facilitated Proton Transfer in Enzyme Catalysis

1968 • 119 citations

Synthetic biochemical models

1970 • 107 citations

Crystal structure of elastase–substrate complex at −55 °C

1976 • 102 citations

Mechanism of action of serine proteases: tetrahedral intermediate and concerted proton transfer

1976 • 93 citations

The Kinetics and Mechanism of Papain-Catalyzed Hydrolyses¹

1966 • 92 citations

Ionic Strength and Protonic Activity of Supercooled Solutions Used in Experiments with Enzyme Systems

1973 • 85 citations

Thiohemiacetal formation by inhibitory aldehydes at the active site of papain

1977 • 83 citations

Mechanism of chymotrypsin. Structure, reactivity, and nonproductive binding relations

1973 • 80 citations

Stereoelectronic control in carbon-oxygen and phosphorus-oxygen bond breaking processes. Ab initio calculations and speculations on the mechanism of action of ribonuclease A, staphylococcal nuclease, and lysozyme

1977 • 75 citations

Reactions of nucleophilic reagents with acylating agents of extreme reactivity and unreactivity. Correlation of .beta. values for attacking and leaving group variation

1970 • 65 citations

Escherichia coli alkaline phosphatase. Relaxation spectra of ligand binding

1972 • 62 citations

Binding sites for substrate leaving groups and added nucleophiles in papain-catalyzed hydrolyses

1969 • 60 citations

A detailed structural comparison between the charge relay system in chymotrypsinogen and in α-chymotrypsin

1976 • 59 citations

The Hydrolysis of Imidate Salts. Stereoelectronic Control in the Cleavage of the Hemiorthoamide Tetrahedral Intermediate

1975 • 50 citations

Inhibition of Papain by N‐Acyl‐aminoacetaldehydes and N‐Acyl‐aminopropanones

1977 • 49 citations

Cryoenzymology: The use of sub-zero temperatures and fluid solutions in the study of enzyme mechanisms

1976 • 48 citations

Conformational isomers of alkaline phosphatase in the mechanism of hydrolysis

1971 • 48 citations

The importance of the conformation of the tetrahedral intermediate for the α‐chymotrypsin‐catalyzed hydrolysis of peptide substrates

1975 • 47 citations

pH-dependence and structure–activity relationships in the papain-catalysed hydrolysis of anilides

1971 • 41 citations

Direct observation of a tetrahedral intermediate during amidine hydrolysis

1970 • 37 citations

Nitrogen isotope effects on the papain-catalyzed hydrolysis of N-benzoyl-L-argininamide

1974 • 34 citations

Cryoenzymology of papain: reaction mechanism with an ester substrate

1978 • 34 citations

The Mechanism of Hydrolysis of Imidate Salts. The Importance of Stereoelectronic Control and pH of the Reaction Medium on the Cleavage of Tetrahedral Intermediates

1975 • 32 citations

Demonstration of a change in the rate-determining step in papain- and ficin-catalyzed acyl-transfer reactions

1971 • 31 citations

Papain-Catalyzed Reactions of Esters with Alcohols. The Nature of the Rate-Determining Step^*

1967 • 27 citations

Papain-catalyzed reactions at subzero temperatures

1976 • 26 citations

Thermodynamics of binding to native α-chymotrypsin and to forms of α-chymotrypsin in which catalytically essential residues are modified; a study of "productive" and "nonproductive" associations

1977 • 24 citations

Effects of substituents on the rates of deacylation of substituted benzoyl papains. Role of a carboxylate residue in the catalytic mechanism

1978 • 24 citations

Pretransition-state protonation and the rate of chymotrypsin catalysis.

1967 • 23 citations

Relaxation spectra of yeast hexokinases. Isomerization of the enzyme

1977 • 10 citations

The mechanism of proton transfers

1972 • 10 citations

Mechanism of proton transfer. II. Transfers to and from imidazole

1973 • 6 citations

[Carbohydrate and nitrogen metabolism of sugar beet].

1947 • 1 citations