Chimeric constructs show that the unique N-terminal domain of the cyclic AMP phosphodiesterase RD1 (RNPDE4A1A; rPDE-IVA1) can confer membrane association upon the normally cytosolic protein chloramphenicol acetyltransferase

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes

1986 • 4,785 citations

Empirical Predictions of Protein Conformation

1978 • 3,167 citations

SV40-transformed simian cells support the replication of early SV40 mutants

1981 • 2,564 citations

CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements

1987 • 1,792 citations

The ancient regulatory-protein family of WD-repeat proteins

1994 • 1,478 citations

Attachment of a 40-base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes.

1989 • 1,295 citations

A conserved tripeptide sorts proteins to peroxisomes.

1989 • 1,113 citations

The signal peptide

1990 • 1,096 citations

A simple phase-extraction assay for chloramphenicol acyltransferase activity

1988 • 1,006 citations

The identification of a new cyclic nucleotide phosphodiesterase activity in human and guinea-pig cardiac ventricle. Implications for the mechanism of action of selective phosphodiesterase inhibitors

1987 • 303 citations

A Family of Human Phosphodiesterases Homologous to the Dunce Learning and Memory Gene Product of Drosophila melanogaster are Potential Targets for Antidepressant Drugs

1993 • 287 citations

Two classes of fatty acid acylated proteins exist in eukaryotic cells.

1985 • 196 citations

The nature of the electrophoretically separable multiple forms of rat liver monoamine oxidase

1973 • 195 citations

The properties and extracellular location of 5′-nucleotidase of the rat fat-cell plasma membrane

1975 • 170 citations

Molecular cloning and expression of human myocardial cGMP-inhibited cAMP phosphodiesterase.

1992 • 158 citations

Insulin triggers cyclic AMP-dependent activation and phosphorylation of a plasma membrane cyclic AMP phosphodiesterase

1980 • 149 citations

Cloning and characterization of mammalian homologs of the Drosophila dunce+ gene.

1989 • 147 citations

Molecular biology of the cyclic AMP-specific cyclic nucleotide phosphodiesterases: A diverse family of regulatory enzymes

1994 • 135 citations

Engineered deletion of the unique N-terminal domain of the cyclic AMP-specific phosphodiesterase RD1 prevents plasma membrane association and the attainment of enhanced thermostability without altering its sensitivity to inhibition by rolipram

1993 • 133 citations

The ratPDE3/IVd phosphodiesterase gene codes for multiple proteins differentially activated by cAMP-dependent protein kinase

1994 • 132 citations

The G protein connection: molecular basis of membrane association

1991 • 114 citations

Characterization of the structure of a low Km, rolipram-sensitive cAMP phosphodiesterase. Mapping of the catalytic domain.

1992 • 113 citations

Differential CNS expression of alternative mRNA isoforms of the mammalian genes encoding cAMP-specific phosphodiesterases

1994 • 108 citations

Purification and properties of the insulin-stimulated cyclic AMP phosphodiesterase from rat liver plasma membranes

1981 • 100 citations

Identification and characterization of the type-IVA cyclic AMP-specific phosphodiesterase RD1 as a membrane-bound protein expressed in cerebellum

1995 • 93 citations

Structure of two rat genes coding for closely related rolipram-sensitive cAMP phosphodiesterases. Multiple mRNA variants originate from alternative splicing and multiple start sites.

1994 • 92 citations

Identification of two splice variant forms of type-IVB cyclic AMP phosphodiesterase, DPD (rPDE-IVB1) and PDE-4 (rPDE-IVB2) in brain: selective localization in membrane and cytosolic compartments and differential expression in various brain regions

1994 • 88 citations

Plasmid cDNA-directed protein synthesis in a coupled eukaryoticin vitrotranscription-translation system

1992 • 74 citations

Identification and characterization of both the cytosolic and particulate forms of cyclic GMP-stimulated cyclic AMP phosphodiesterase from rat liver

1986 • 73 citations

The insulin- and glucagon-stimulated ‘dense-vesicle’ high-affinity cyclic AMP phosphodiesterase from rat liver. Purification, characterization and inhibitor sensitivity

1987 • 72 citations

Molecular cloninc and expression, in both COS-1 cells and S. cerevisiae, of a human cytosolic type-IVA cyclic AMP specific phosphodiesterase (hPDE-IVA-h6.1)

1994 • 46 citations

Indirect immunofluorescence localization of β-adrenergic receptors and G-proteins in human A431 cells

1989 • 45 citations

Purification, characterization and analysis of rolipram inhibition of a human type-IVA cyclic AMP-specific phosphodiesterase expressed in yeast

1994 • 45 citations

Activation and phosphorylation of the ‘dense-vesicle’ high-affinity cyclic AMP phosphodiesterase by cyclic AMP-dependent protein kinase

1989 • 41 citations

Studies on the cellular location, physical properties and endogenously attached lipids of acylated proteins in human squamous-carcinoma cell lines

1987 • 28 citations

Alternative splicing of the type-IVA cyclic AMP phosphodiesterase gene provides isoform variants with distinct N-terminal domains fused to a common, soluble catalytic unit: ‘designer’ changes in Vmax, stability and membrane association

1995 • 14 citations