The SNF5 protein of Saccharomyces cerevisiae is a glutamine- and proline-rich transcriptional activator that affects expression of a broad spectrum of genes.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

Experiments in molecular genetics

1972 • 28,260 citations

Molecular Cloning. A Laboratory Manual

1983 • 27,328 citations

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

1977 • 12,015 citations

Improved tools for biological sequence comparison.

1988 • 11,455 citations

Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

1980 • 8,711 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Methods in yeast genetics

1979 • 7,824 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis

1981 • 2,828 citations

Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis

1983 • 2,609 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system

1981 • 1,991 citations

Analysis of Sp1 in vivo reveals mutiple transcriptional domains, including a novel glutamine-rich activation motif

1988 • 1,403 citations

[10] Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast

1983 • 1,095 citations

Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experiments

1979 • 967 citations

Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of Yeast

1986 • 946 citations

Deletion analysis of GAL4 defines two transcriptional activating segments

1987 • 898 citations

Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression.

1988 • 830 citations

The proline-rich transcriptional activator of CTF/NF-I is distinct from the replication and DNA binding domain

1989 • 781 citations

Molecular Characterization of a Functional cDNA Encoding the Serotonin 1c Receptor

1988 • 691 citations

A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor

1985 • 686 citations

Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions

1986 • 623 citations

Structure of a yeast pheromone gene (MFα): A putative α-factor precursor contains four tandem copies of mature α-factor

1982 • 587 citations

Cloning and expression of AP-2, a cell-type-specific transcription factor that activates inducible enhancer elements.

1988 • 578 citations

GENES AFFECTING THE REGULATION OF SUC2 GENE EXPRESSION BY GLUCOSE REPRESSION IN SACCHAROMYCES CEREVISIAE

1984 • 567 citations

GCN4 protein, synthesize in vitro, binds HIS3 regulatory sequences: Implications for general control of amino acid biosynthetic genes in yeast

1985 • 496 citations

A cloned octamer transcription factor stimulates transcription from lymphoid–specific promoters in non–B cells

1988 • 494 citations

DNA specificity of the bicoid activator protein is determined by homeodomain recognition helix residue 9

1989 • 487 citations

[76] β-d-Fructofuranoside fructohydrolase from yeast

1975 • 455 citations

Identification and characterization of HAP4: a third component of the CCAAT-bound HAP2/HAP3 heteromer.

1989 • 432 citations

The B-cell-specific Oct-2 protein contains POU box- and homeo box-type domains.

1988 • 429 citations

Isolation of the β-tubulin gene from yeast and demonstration of its essential function in vivo

1983 • 409 citations

Yeast α factor is processed from a larger precursor polypeptide: The essential role of a membrane-bound dipeptidyl aminopeptidase

1983 • 403 citations

A human lymphoid- specific transcription factor that activates immunoglobulin genes is a homoeobox protein

1988 • 399 citations

Saccharomyces cerevisiae GAL1-GAL10 Divergent Promoter Region: Location and Function of the Upstream Activating Sequence UAS_G

1984 • 395 citations

A complex formed between cell components and an HSV structural polypeptide binds to a viral immediate early gene regulatory DNA sequence

1988 • 360 citations

MUTATIONS AFFECTING TY-MEDIATED EXPRESSION OF THE HIS4 GENE OF SACCHAROMYCES CEREVISIAE

1984 • 355 citations

Isolation and Genetic Analysis of Saccharomyces cerevisiae Mutants Supersensitive to G1 Arrest by a Factor and α Factor Pheromones

1982 • 322 citations

Evidence of DNA: protein interactions that mediate HSV-1 immediate early gene activation by VP16.

1988 • 289 citations

Molecular mechanisms of transcriptional regulation in yeast

1990 • 224 citations

DNA-bound Fos proteins activate transcription in yeast

1988 • 223 citations

Binding of the virion protein mediating alpha gene induction in herpes simplex virus 1-infected cells to its cis site requires cellular proteins.

1987 • 217 citations

The Saccharomyces cerevisiae BAR1 gene encodes an exported protein with homology to pepsin.

1988 • 212 citations

The yeast SNF3 gene encodes a glucose transporter homologous to the mammalian protein.

1988 • 205 citations

Control of yeast cell type by the mating type locus

1981 • 180 citations

The SPT6 gene is essential for growth and is required for delta-mediated transcription in Saccharomyces cerevisiae.

1987 • 132 citations

LexA protein is a repressor of the colicin E1 gene.

1983 • 126 citations

Molecular analysis of SNF2 and SNF5, genes required for expression of glucose-repressible genes in Saccharomyces cerevisiae.

1986 • 123 citations

The CCR4 gene from Saccharomyces cerevisiae is required for both nonfermentative and spt-mediated gene expression.

1990 • 113 citations

Association of charge clusters with functional domains of cellular transcription factors.

1989 • 100 citations

Upstream region required for regulated expression of the glucose-repressible SUC2 gene of Saccharomyces cerevisiae.

1984 • 93 citations

SSN20 is an essential gene with mutant alleles that suppress defects in SUC2 transcription in Saccharomyces cerevisiae.

1987 • 91 citations

Analysis of mutations affecting Ty-mediated gene expression in Saccharomyces cerevisiae

1981 • 83 citations

SUPPRESSORS OF snf 2 MUTATIONS RESTORE INVERTASE DEREPRESSION AND CAUSE TEMPERATURE-SENSITIVE LETHALITY IN YEAST

1986 • 78 citations

SPT6, an essential gene that affects transcription in Saccharomyces cerevisiae, encodes a nuclear protein with an extremely acidic amino terminus.

1990 • 77 citations

SNF6 encodes a nuclear protein that is required for expression of many genes in Saccharomyces cerevisiae.

1990 • 68 citations

Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis

1984 • 60 citations

Comparison of two yeast invertase genes: conservation of the upstream regulatory region

1985 • 50 citations

Consequences of growth media, gene copy number, and regulatory mutations on the expression of the PRB1 gene of Saccharomyces cerevisiae.

1990 • 42 citations

Molecular Mechanisms Of Transcriptional Regulation In Yeast

1989 • 22 citations