Using Recombinant DNA Techniques to Study Protein Targeting in the Eucaryotic Cell

A new technique for the assay of infectivity of human adenovirus 5 DNA

1973 • 9,740 citations

SV40-transformed simian cells support the replication of early SV40 mutants

1981 • 2,564 citations

A short amino acid sequence able to specify nuclear location

1984 • 2,529 citations

Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 Å resolution

1981 • 2,490 citations

Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus

1983 • 1,857 citations

Functional messenger RNAs are produced by SP6in vitrotranscription of cloned cDNAs

1984 • 1,533 citations

Transformation of mammalian cells with genes from procaryotes and eucaryotes

1979 • 1,474 citations

Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells

1977 • 1,411 citations

Sequence requirements for nuclear location of simian virus 40 large-T antigen

1984 • 1,278 citations

Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components.

1975 • 1,175 citations

Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.

1981 • 1,048 citations

Construction and applications of a highly transmissible murine retrovirus shuttle vector

1984 • 1,020 citations

Characterization of DNA sequences through which cadmium and glucocorticoid hormones induce human metallothionein-IIA gene

1984 • 955 citations

Mechanisms for the incorporation of proteins in membranes and organelles.

1982 • 875 citations

INTRACELLULAR TRANSPORT OF SECRETORY PROTEINS IN THE PANCREATIC EXOCRINE CELL

1967 • 841 citations

Protein translocation across the endoplasmic reticulum

1984 • 825 citations

Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes.

1981 • 807 citations

Order of events in the yeast secretory pathway

1981 • 804 citations

Compilation of published signal sequences

1984 • 784 citations

Chloroquine inhibits lysosomal enzyme pinocytosis and enhances lysosomal enzyme secretion by impairing receptor recycling.

1980 • 743 citations

The class II molecules of the human and murine major histocompatibility complex

1984 • 664 citations

Two mRNAs with different 3′ ends encode membrane-bound and secreted forms of immunoglobulin μ chain

1980 • 659 citations

Secretory protein translocation across membranes—the role of the ‘docking protein’

1982 • 648 citations

Internalization and processing of transferrin and the transferrin receptor in human carcinoma A431 cells.

1983 • 636 citations

Immunocytochemical localization of galactosyltransferase in HeLa cells: codistribution with thiamine pyrophosphatase in trans-Golgi cisternae.

1982 • 617 citations

Post-Translational Proteolysis in Polypeptide Hormone Biosynthesis

1982 • 590 citations

IMPORT OF PROTEINS INTO MITOCHONDRIA

1991 • 583 citations

Synthesis of secreted and membrane-bound immunoglobulin mu heavy chains is directed by mRNAs that differ at their 3′ ends

1980 • 562 citations

A polypeptide domain that specifies migration of nucleoplasmin into the nucleus

1982 • 556 citations

DNA Tumor Viruses

1982 • 550 citations

Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae.

1981 • 542 citations

Pre- and post-golgi vacuoles operate in the transport of semliki forest virus membrane glycoproteins to the cell surface

1984 • 523 citations

POLYPROTEIN GENE EXPRESSION: Generation of Diversity of Neuroendocrine Peptides

1984 • 497 citations

Filamentous bacterial viruses

1969 • 454 citations

Hepatoma secretory proteins migrate from rough endoplasmic reticulum to Golgi at characteristic rates

1983 • 436 citations

Translocation of proteins across the endoplasmic reticulum. II. Signal recognition protein (SRP) mediates the selective binding to microsomal membranes of in-vitro-assembled polysomes synthesizing secretory protein.

1981 • 414 citations

Secretion and Membrane Localization of Proteins inEscherichia Coli

1980 • 402 citations

Protein translocation across the endoplasmic reticulum. I. Detection in the microsomal membrane of a receptor for the signal recognition particle.

1982 • 388 citations

Targeting of E. coli β-galactosidase to the nucleus in yeast

1984 • 386 citations

The genome of human papovavirus BKV

1979 • 378 citations

Primary structure of human transferrin receptor deduced from the mRNA sequence

1984 • 351 citations

Expressing a human proinsulin cDNA in a mouse ACTH-secreting cell. Intracellular storage, proteolytic processing, and secretion on stimulation

1983 • 329 citations

Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase

1985 • 327 citations

How are proteins imported into mitochondria?

1983 • 307 citations

Mechanisms of protein localization

1983 • 282 citations

Recombinant antibodies possessing novel effector functions

1984 • 282 citations

Mutations which alter the function of the signal sequence of the maltose binding protein of Escherichia coli

1980 • 268 citations

Expression from cloned cDNA of cell-surface secreted forms of the glycoprotein of vesicular stomatitis virus in eucaryotic cells

1982 • 266 citations

Altered cytoplasmic domains affect intracellular transport of the vesicular stomatitis virus glycoprotein

1983 • 264 citations

Nucleotide sequence of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase, a glycoprotein of endoplasmic reticulum

1984 • 263 citations

Protein migration into nuclei. I. Frog oocyte nuclei in vivo accumulate microinjected histones, allow entry to small proteins, and exclude large proteins.

1975 • 262 citations

Glucocorticoid-stimulated accumulation of mouse mammary tumor virus RNA: increased rate of synthesis of viral RNA.

1977 • 256 citations

The nonglycosylated glycoprotein of vesicular stomatitis virus is temperature-sensitive and undergoes intracellular aggregation at elevated temperatures.

1979 • 255 citations

A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences.

1984 • 251 citations

Sorting of an apical plasma membrane glycoprotein occurs before it reaches the cell surface in cultured epithelial cells.

1984 • 247 citations

Surface distribution and recycling of the low density lipoprotein receptor as visualized with antireceptor antibodies.

1982 • 240 citations

Role of positive charge on the amino-terminal region of the signal peptide in protein secretion across the membrane.

1982 • 234 citations

Initiation of translation at internal AUG codons in mammalian cells

1984 • 231 citations

The Genetic Control of HLA‐A and B Antigens in Somatic Cell Hybrids: Requirement for β₂ Microglobulin

1978 • 222 citations

Identification of the sequence responsible for the nuclear accumulation of the influenza virus nucleoprotein in Xenopus oocytes

1985 • 220 citations

Sequence and topology of a model intracellular membrane protein, E1 glycoprotein, from a coronavirus

1984 • 210 citations

Evidence for a glycoprotein "signal" involved in transport between subcellular organelles. Two membrane glycoproteins encoded by murine leukemia virus reach the cell surface at different rates.

1982 • 209 citations

Evidence that murine pre-B cells synthesise μ heavy chains but no light chains

1979 • 208 citations

Passage of viral membrane proteins through the Golgi complex

1981 • 205 citations

Molecular cloning and sequencing of cDNA encoding the precursor to the small subunit of chloroplast ribulose-1,5-bisphosphate carboxylase

1980 • 202 citations

The cleavable prepiece of an imported mitochondrial protein is sufficient to direct cytosolic dihydrofolate reductase into the mitochondrial matrix

1984 • 199 citations

Chloroquine diverts ACTH from a regulated to a constitutive secretory pathway in AtT-20 cells

1983 • 198 citations

A 70-kd protein of the yeast mitochondrial outer membrane is targeted and anchored via its extreme amino terminus.

1984 • 198 citations

Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins.

1975 • 196 citations

Sequence analysis of mutations that prevent export of λ receptor, an Escherichia coli outer membrane protein

1980 • 195 citations

Effect of Tunicamycin on IgM, IgA, and IgG Secretion by Mouse Plasmacytoma Cells

1978 • 180 citations

Expression of a preproinsulin-beta-galactosidase gene fusion in mammalian cells.

1983 • 178 citations

cDNA Cloning and In Vitro Transcription of the Complete Brome Mosaic Virus Genome

1984 • 176 citations

The presence of cysteine in the cytoplasmic domain of the vesicular stomatitis virus glycoprotein is required for palmitate addition.

1984 • 174 citations

Molecular abnormality of human alpha1-antitrypsin variant (Pi-ZZ) associated with plasma activity deficiency.

1976 • 172 citations

Regulation of a metallothionein-growth hormone hybrid gene in bovine papilloma virus.

1983 • 168 citations

Rapid endocytosis of the transferrin receptor in the absence of bound transferrin.

1985 • 167 citations

Short-Term, High-Efficiency Expression of Transfected DNA

1984 • 166 citations

cDNA clone for the human invariant gamma chain of class II histocompatibility antigens and its implications for the protein structure.

1983 • 166 citations

Yeast genes fused to beta-galactosidase in Escherichia coli can be expressed normally in yeast.

1981 • 165 citations

Nuclear transplantation and the control of gene activity in animal development

1970 • 165 citations

Four secretory proteins synthesized by hepatocytes are transported from endoplasmic reticulum to Golgi complex at different rates.

1984 • 164 citations

Nucleocytoplasmic segregation of proteins and RNAs

1983 • 163 citations

Transient involvement of signal recognition particle and its receptor in the microsomal membrane prior to protein translocation

1983 • 160 citations

A single amino acid substitution in a hydrophobic domain causes temperature-sensitive cell-surface transport of a mutant viral glycoprotein

1985 • 158 citations

Structure of genes for membrane and secreted murine IgD heavy chains

1982 • 158 citations

Biogenesis of epithelial cell polarity: Intracellular sorting and vectorial exocytosis of an apical plasma membrane glycoprotein

1984 • 157 citations

Mechanisms of integration of de novo-synthesized polypeptides into membranes: signal-recognition particle is required for integration into microsomal membranes of calcium ATPase and of lens MP26 but not of cytochrome b5.

1983 • 155 citations

Importance of secondary structure in the signal sequence for protein secretion.

1983 • 152 citations

Simple, efficientin vitrosynthesis of capped RNA useful for direct expression of cloned eukaryoti genes

1982 • 152 citations

The effect of carbohydrate depletion on the properties of yeast external invertase.

1978 • 151 citations

Lysosomal enzyme phosphorylation. Recognition of a protein-dependent determinant allows specific phosphorylation of oligosaccharides present on lysosomal enzymes.

1984 • 150 citations

Yeast mitochondrial outer membrane specifically binds cytoplasmically-synthesized precursors of mitochondrial proteins

1983 • 149 citations

A stop transfer sequence confers predictable transmembrane orientation to a previously secreted protein in cell-free systems

1983 • 142 citations

A NONDEFECTIVE (COMPETENT) ADENOVIRUS-SV40 HYBRID ISOLATED FROM THE AD.2-SV40 HYBRID POPULATION

1969 • 141 citations

Studies on the size, chemical composition, and partial sequence of the neuraminidase (NA) from type A influenza viruses show that the N-terminal region of the NA is not processed and serves to anchor the NA in the viral membrane

1982 • 134 citations

Mechanism of mRNA Recognition by Eukaryotic Ribosomes During Initiation of Protein Synthesis

1981 • 134 citations

Influenza virus hemagglutinin expression is polarized in cells infected with recombinant SV40 viruses carrying cloned hemagglutinin DNA

1983 • 132 citations

Export of proteins from oocytes of Xenopus laevis

1979 • 131 citations

Subunit structure, cell surface orientation, and partial amino-acid sequences of murine histocompatibility antigens.

1976 • 128 citations

The complete sequence of the mRNA for the HLA-DR-associated invariant chain reveals a polypeptide with an unusual transmembrane polarity.

1984 • 124 citations

Rat liver asialoglycoprotein receptor lacks a cleavable NH2-terminal signal sequence.

1984 • 124 citations

Conversion of a secretory protein into a transmembrane protein results in its transport to the golgi complex but not to the cell surface

1984 • 120 citations

Signal recognition particle is required for co-translational insertion of cytochrome P-450 into microsomal membranes.

1984 • 118 citations

Export of major cell surface proteins is blocked in yeast secretory mutants

1983 • 117 citations

Amphotropic retrovirus vector system for human cell gene transfer.

1984 • 117 citations

Spatial relationships of the proteins of vesicular stomatitis virus: induction of reversible oligomers by cleavable protein cross-linkers and oxidation

1977 • 116 citations

Studies on Nondefective Adenovirus-Simian Virus 40 Hybrid Viruses I. A Newly Characterized Simian Virus 40 Antigen Induced by the Ad2 ⁺ ND ₁ Virus

1971 • 112 citations

A deletion that includes the signal peptidase cleavage site impairs processing, glycosylation, and secretion of cell surface yeast acid phosphatase.

1984 • 112 citations

Intragenic suppressor mutations that restore export of maltose binding protein with a truncated signal peptide

1984 • 111 citations

Import of proteins into mitochondria: nucleotide sequence of the gene for a 70-kd protein of the yeast mitochondrial outer membrane.

1983 • 110 citations

Mutations of the Rous sarcoma virus env gene that affect the transport and subcellular location of the glycoprotein products.

1984 • 109 citations

Vesicular stomatitis virus glycoprotein, albumin, and transferrin are transported to the cell surface via the same Golgi vesicles.

1983 • 107 citations

Translation of insulin-related polypeptides from messenger RNAs with tandemly reiterated copies of the ribosome binding site

1983 • 105 citations

Inducible expression of amplified human beta interferon genes in CHO cells.

1984 • 99 citations

A prokaryotic membrane anchor sequence: carboxyl terminus of bacteriophage f1 gene III protein retains it in the membrane.

1982 • 94 citations

Cell surface expression of the influenza virus hemagglutinin requires the hydrophobic carboxy-terminal sequences

1982 • 94 citations

Domain structure of bacteriophage fd adsorption protein

1981 • 94 citations

Structure of the altered oligosaccharide present in glycoproteins from a clone of Chinese hamster ovary cells deficient in N-acetylglucosaminyltransferase activity.

1978 • 93 citations

Allospecific and virus-specific cytolytic T lymphocytes are restricted to the N or C1 domain of H-2 antigens expressed on L cells after DNA-mediated gene transfer.

1983 • 92 citations

Complete nucleotide sequence of the neuraminidase gene of human influenza virus A/WSN/33

1982 • 91 citations

Segregation of mutant ovalbumins and ovalbumin-globin fusion proteins in Xenopus oocytes

1984 • 89 citations

The signal sequence of ovalbumin is located near the NH2 terminus.

1982 • 88 citations

Solubilization and properties of galactosyltransferase and sulfotransferase activities of Golgi membranes in Triton X-100.

1978 • 86 citations

Construction, expression and recognition of an H—2 molecule lacking its carboxyl terminus

1984 • 84 citations

Cathepsin D and beta-hexosaminidase synthesized in the presence of 1-deoxynojirimycin accumulate in the endoplasmic reticulum.

1984 • 84 citations

The appearance and internalization of transferrin receptors at the margins of spreading human tumor cells

1985 • 82 citations

Cytolytic T cells recognize the two amino-terminal domains of H-2 K antigens in tandem in influenza A infected cells

1984 • 82 citations

Intracellular transport and secretion of hepatitis B surface antigen in mammalian cells

1984 • 81 citations

Transfer of a cloned immunoglobulin light-chain gene to mutant hybridoma cells restores specific antibody production

1983 • 78 citations

α ₁ -Antitrypsin: The Presence of Excess Mannose in the Z Variant Isolated from Liver

1978 • 74 citations

An ‘internal” signal sequence directs secretion and processing of proinsulin in bacteria

1981 • 68 citations

Nucleotide sequences of gene segments encoding membrane domains of immunoglobulin gamma chains.

1982 • 67 citations

Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses VIII. Association of Simian Virus 40 Transplantation Antigen with a Specific Region of the Early Viral Genome

1973 • 62 citations

Transport and topology of galactosyltransferase in endomembranes of HeLa cells.

1983 • 58 citations

Requirement for Signal Peptide Cleavage of Escherichia coli Prolipoprotein

1983 • 57 citations

Synthetic pre-proparathyroid hormone leader sequence inhibits cell-free processing of placental, parathyroid, and pituitary prehormones.

1980 • 52 citations

Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone.

1984 • 52 citations

Physical properties of a soluble form of the glycoprotein of vesicular stomatitis virus at neutral and acidic pH

1983 • 49 citations

Effects of replacing serine and threonine residues within the signal peptide on the secretion of the major outer membrane lipoprotein of Escherichia coli.

1984 • 47 citations

Ovalbumin utilizes an NH2-terminal signal sequence.

1982 • 45 citations

Beta 2-microglobulin induces intracellular transport of human class I transplantation antigen heavy chains in Xenopus laevis oocytes.

1984 • 43 citations

Efficient expression of cloned complementary DNAs for secretory proteins after injection into Xenopus oocytes

1984 • 41 citations

Glycosylation and surface expression of the influenza virus neuraminidase requires the N-terminal hydrophobic region.

1984 • 40 citations

Defects in functional expression of an influenza virus hemagglutinin lacking the signal peptide sequences.

1983 • 40 citations

Pushing the signal hypothesis: what are the limits?

1985 • 37 citations

Demonstration of Specific Receptors of the Rough Endoplasmic Membrane for the Signal Sequence of Carp Preproinsulin

1980 • 36 citations

Effects of mutations at glycine residues in the hydrophobic region of the Escherichia coli prolipoprotein signal peptide on the secretion across the membrane.

1984 • 32 citations

Chimeric influenza virus hemagglutinin containing either the NH2 terminus or the COOH terminus of G protein of vesicular stomatitis virus is defective in transport to the cell surface.

1984 • 32 citations

Restriction of docking protein to the rough endoplasmic reticulum: immunocytochemical localization in rat liver.

1985 • 31 citations

Transport of an uncleaved preprotein into the endoplasmic reticulum of rat pituitary cells.

1981 • 30 citations

Non-parallel kinetics and the role of tissue-specific factors in the secretion of chicken ovalbumin and lysozyme from Xenopus oocytes

1981 • 25 citations

Expression of Herpes simplex virus type 1 glycoprotein C antigens in Escherichia coli

1984 • 24 citations

Human histocompatibility antigen mutants immunoselected in vitro. Biochemical analysis of a mutant which synthesizes an altered HLA-A2 heavy chain.

1982 • 20 citations

Influenza virus hemagglutinin containing an altered hydrophobic carboxy terminus accumulates intracellularly

1984 • 20 citations

Synthetic leader peptide modulates secretion of proteins from microinjected Xenopus oocytes.

1983 • 19 citations

The structure of the mouse immunoglobulin in γ₃membrane gene segment

1983 • 18 citations

Construction of a synthetic messenger RNA encoding a membrane protein.

1983 • 7 citations

The Oocyte as a Secretory Cell

1983 • 3 citations