GAL4 Protein: Purification, Association with GAL80 Protein, and Conserved Domain Structure

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

A comprehensive set of sequence analysis programs for the VAX

1984 • 14,824 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Rapid and Sensitive Protein Similarity Searches

1985 • 4,091 citations

Silver staining of proteins in polyacrylamide gels

1981 • 3,565 citations

Affinity purification of sequence-specific DNA binding proteins.

1986 • 1,255 citations

Five intermediate complexes in transcription initiation by RNA polymerase II

1989 • 1,033 citations

Sequences That Regulate the Divergent GAL1-GAL10 Promoter in Saccharomyces cerevisiae

1984 • 955 citations

Deletion analysis of GAL4 defines two transcriptional activating segments

1987 • 898 citations

Yeast heat shock factor is an essential DNA-binding protein that exhibits temperature-dependent phosphorylation

1988 • 886 citations

O-glycosylation of eukaryotic transcription factors: Implications for mechanisms of transcriptional regulation

1988 • 884 citations

Zinc fingers: Gilt by association

1988 • 799 citations

Specific DNA binding of GAL4, a positive regulatory protein of yeast

1985 • 767 citations

Immunogenic structure of the influenza virus hemagglutinin

1982 • 759 citations

A model fungal gene regulatory mechanism: the GAL genes of Saccharomyces cerevisiae

1987 • 676 citations

Two DNA-binding factors recognize specific sequences at silencers, upstream activating sequences, autonomously replicating sequences, and telomeres in Saccharomyces cerevisiae.

1988 • 511 citations

Tests for comparing related amino-acid sequences. Cytochrome c and cytochrome c551

1971 • 440 citations

The yeast UASG is a transcriptional enhancer in human hela cells in the presence of the GAL4 trans-activator

1988 • 362 citations

GAL4 activates gene expression in mammalian cells

1988 • 355 citations

The carboxy-terminal 30 amino acids of GAL4 are recognized by GAL80

1987 • 349 citations

An amino-terminal fragment of GAL4 binds DNA as a dimer

1989 • 296 citations

Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization.

1984 • 284 citations

Connections Between Transcriptional Activators, Silencers, and Telomeres as Revealed by Functional Analysis of a Yeast DNA-Binding Protein

1988 • 284 citations

Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon.

1982 • 280 citations

The multidrug resistance gene PDR1 from Saccharomyces cerevisiae.

1987 • 276 citations

Primary Structure of the Saccharomyces cerevisiae GAL4 Gene

1984 • 276 citations

GAL4 derivatives function alone and synergistically with mammalian activators in vitro

1988 • 274 citations

Statistical positioning of nucleosomes by specific protein-binding to an upstream activating sequence in yeast

1988 • 270 citations

Specific protein binding to far upstream activating sequences in polymerase II promoters.

1985 • 246 citations

A yeast protein that influences the chromatin structure of UASG and functions as a powerful auxiliary gene activator.

1990 • 229 citations

Interaction of positive and negative regulatory proteins in the galactose regulon of yeast

1987 • 226 citations

Activation of yeast polymerase II transcription by herpesvirus VP16 and GAL4 derivatives in vitro.

1989 • 187 citations

Genetic evidence that zinc is an essential co-factor in the DNA binding domain of GAL4 protein

1987 • 169 citations

Interaction of GAL4 and GAL80 gene regulatory proteins in vitro.

1987 • 161 citations

Analysis of theKluyveromyces lactispositive regulatory geneLAC9reveals functional homology to, but sequence divergence from, theSaccharomyces cerevisiae GALAgene

1986 • 158 citations

Yeast regulatory gene PPR1

1984 • 141 citations

Characterization of a Positive Regulatory Gene, LAC9, That Controls Induction of the Lactose-Galactose Regulon of Kluyveromyces lactis: Structural and Functional Relationships to GAL4 of Saccharomyces cerevisiae

1987 • 128 citations

In vivo DNA-binding properties of a yeast transcription activator protein.

1987 • 112 citations

Regulated phosphorylation and dephosphorylation of GAL4, a transcriptional activator.

1989 • 106 citations

Functional domains of the yeast regulatory protein GAL4.

1986 • 95 citations

A single glutamic acid residue plays a key role in the transcriptional activation function of lambda repressor

1989 • 94 citations

Yeast regulatory gene GAL3: carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases.

1988 • 94 citations

Structure and function of the Zn(II) binding site within the DNA-binding domain of the GAL4 transcription factor.

1989 • 89 citations

LEU3 of Saccharomyces cerevisiae encodes a factor for control of RNA levels of a group of leucine-specific genes.

1987 • 87 citations

A method for multiple sequence alignment with gaps

1989 • 80 citations

Regulated overproduction of the GAL4 gene product greatly increases expression from galactose-inducible promoters on multi-copy expression vectors in yeast

1987 • 79 citations

Isolation and characterization of the positively acting regulatory geneQUTAfromAspergillus nidulans

1987 • 66 citations

Purification and characterization of the yeast transcriptional activator GAL4.

1990 • 66 citations

Nucleotide sequence of the yeast regulatory geneGAL80

1984 • 66 citations

Mutational analysis of the GAL4-encoded transcriptional activator protein of Saccharomyces cerevisiae.

1988 • 66 citations

Altering DNA-binding specificity of GAL4 requires sequences adjacent to the zinc finger

1989 • 63 citations

No strict alignment is required between a transcriptional activator binding site and the "TATA box" of a yeast gene.

1988 • 56 citations

GAL4 of Saccharomyces cerevisiae activates the lactose-galactose regulon of Kluyveromyces lactis and creates a new phenotype: glucose repression of the regulon.

1987 • 52 citations

Controlled transcription of the yeast regulatory gene GAL80

1985 • 50 citations

Identification of two proteins encoded by the Saccharomyces cerevisiae GAL4 gene.

1984 • 46 citations

Saccharomyces cerevisiae Positive Regulatory Gene PET111 Encodes a Mitochondrial Protein That Is Translated from an mRNA with a Long 5′ Leader

1987 • 45 citations

Functional domains of a negative regulatory protein, GAL80, of Saccharomyces cerevisiae.

1989 • 42 citations

A large internal deletion converts yeast LEU3 to a constitutive transcriptional activator.

1989 • 42 citations

A region flanking the GAL7 gene and a binding site for GAL4 protein as upstream activating sequences in yeast

1985 • 39 citations

GAL3 gene product is required for maintenance of the induced state of the GAL cluster genes in Saccharomyces cerevisiae

1986 • 25 citations