Fluorescence Lifetime Imaging Microscopy (FLIM): Instrumentation and Applications

Two-Photon Laser Scanning Fluorescence Microscopy

1990 • 9,129 citations

Green Fluorescent Protein as a Marker for Gene Expression

1994 • 6,572 citations

Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin

1997 • 3,163 citations

Fluorescence Energy Transfer as a Spectroscopic Ruler

1978 • 2,370 citations

Crystal Structure of the Aequorea victoria Green Fluorescent Protein

1996 • 2,167 citations

Handbook of Biological Confocal Microscopy

1995 • 1,850 citations

Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer

1996 • 1,462 citations

Resonance Energy Transfer: Methods and Applications

1994 • 1,245 citations

Experimentelle und theoretische Untersuchung des zwischenmolekularen Übergangs von Elektronenanregungsenergie

1949 • 977 citations

Fluorescence resonance energy transfer

1995 • 878 citations

Fluorescence lifetime imaging of free and protein-bound NADH.

1992 • 722 citations

MEASUREMENTS OF SUBNANOSECOND FLUORESCENCE LIFETIMES WITH A CROSS‐CORRELATION PHASE FLUOROMETER*

1969 • 600 citations

Oligomerization of epidermal growth factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy. A stereochemical model for tyrosine kinase receptor activation.

1995 • 432 citations

The Measurement and Analysis of Heterogeneous Emissions by Multifrequency Phase and Modulation Fluorometry

1984 • 407 citations

Fluorescence lifetime imaging microscopy (FLIM): Spatial resolution of microstructures on the nanosecond time scale

1993 • 364 citations

Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein

1996 • 300 citations

Fluorescence imaging spectroscopy and microscopy

1997 • 289 citations

Resolution of the fluorescence lifetimes in a heterogeneous system by phase and modulation measurements

1981 • 265 citations

Lifetime-selective fluorescence imaging using an rf phase-sensitive camera

1991 • 251 citations

Time resolved imaging microscopy. Phosphorescence and delayed fluorescence imaging

1991 • 236 citations

Luminescence Digital Imaging Microscopy

1989 • 214 citations

An Improved Green Fluorescent Protein Gene as a Vital Marker in Plants

1996 • 211 citations

Optical microscopy for biology

1991 • 197 citations

Fluorescence lifetime imaging using a confocal laser scanning microscope

1992 • 188 citations

Microspectroscopic imaging tracks the intracellular processing of a signal transduction protein: fluorescent-labeled protein kinase C beta I.

1996 • 167 citations

Time‐resolved fluorescence imaging of europium chelate label in immunohistochemistry and in situ hybridization

1992 • 154 citations

Ultrashort Laser Pulses

1993 • 149 citations

A Two-Dimensional Fluorescence Lifetime Imaging System Using a Gated Image Intensifier

1991 • 147 citations

Fluorescence lifetime imaging of oxygen in living cells

1997 • 147 citations

Time-resolved fluorescence microscopy using two-photon excitation

1995 • 130 citations

Fluorescence Lifetime Imaging Microscopy (FLIM): Instrumentation and Applications

1992 • 129 citations

Fluorescence lifetime imaging microscopy (flimscopy). Methodology development and application to studies of endosome fusion in single cells

1993 • 128 citations

Glycolipid migration from the apical to the equatorial subdomains of the sperm head plasma membrane precedes the acrosome reaction Evidence for a primary capacitation event in boar spermatozoa

1995 • 120 citations

Time‐gated fluorescence lifetime imaging and microvolume spectroscopy using two‐photon excitation

1998 • 116 citations

Rapid acquisition, analysis, and display of fluorescence lifetime-resolved images for real-time applications

1997 • 109 citations

Fluorescence lifetime imaging by asynchronous pump-probe microscopy

1995 • 107 citations

Cell Structure and Function by Microspectrofluorometry

1989 • 106 citations

Multifrequency cross-correlation phase fluorometer using synchrotron radiation

1984 • 97 citations

Time-resolved fluorescence lifetime imaging microscopy using a picosecond pulsed tunable dye laser system

1996 • 94 citations

Time-resolved delayed luminescence image microscopy using an europium ion chelate complex

1994 • 91 citations

Inorganic phosphors as new luminescent labels for immunocytochemistry and time‐resolved microscopy

1990 • 90 citations

Fluorescence Spectroscopy, New Methods and Applications

1993 • 82 citations

Time‐resolved fluorescence microscopy using two‐photon excitation

1995 • 82 citations

<title>Time-resolved fluorescence imaging and background rejection by two-photon excitation in laser-scanning microscopy</title>

1992 • 80 citations

Oxygen flux fluorescence lifetime imaging

1997 • 71 citations

Protoplasts transiently expressing the 200K coding sequence of cowpea mosaic virus B-RNA support replication of M-RNA

1993 • 71 citations

Fluorescence lifetime imaging microscopy: Pixel-by-pixel analysis of phase-modulation data

1994 • 69 citations

TIME‐GATED FLUORESCENCE IMAGING FOR THE DIAGNOSIS OF TUMORS IN A MURINE MODEL

1993 • 62 citations

[30] Fluorescence lifetime imaging microscopy: Homodyne technique using high-speed gated image intensifier

1994 • 61 citations

Time-resolved in-vivo fluorescence of photosensitizing porphyrins

1993 • 57 citations

Microscope Phase Fluorometer for Determining the Fluorescence Lifetimes of Fluorochromes

1959 • 55 citations

A Fluorescence Lifetime Distribution Measurement System Based on Phase-Resolved Detection Using an Image Dissector Tube

1989 • 52 citations

Prospects for confocal imaging based on nanosecond fluorescence decay time

1992 • 52 citations

Light quenching and fluorescence depolarization of rhodamine B and applications of this phenomenon to biophysics

1994 • 50 citations

Fluorescence Lifetime Imaging: An Approach for Fuel Equivalence Ratio Imaging

1991 • 48 citations

Fluorescence lifetime imaging microscopy: Pixel‐by‐pixel analysis of phase‐modulation data

1994 • 47 citations

<title>Time-resolved imaging fluorescence microscopy</title>

1992 • 46 citations

Two-Dimensional Gas-Phase Temperature Measurements Using Fluorescence Lifetime Imaging

1996 • 44 citations

Preparation and microscopic visualization of multicolor luminescent immunophosphors

1992 • 42 citations

Fluorescence lifetime imaging of free calcium in single cells

1994 • 41 citations

Proposed standard for image cytometry data files

1990 • 38 citations

Fluorescent Biomolecules: Methodologies And Applications

2012 • 38 citations

Endoscopic tissue characterization by frequency-domain fluorescence lifetime imaging (FD-FLIM)

1997 • 37 citations

Development of long-lifetime metal-ligand probes for biophysics and cellular imaging

1997 • 29 citations

Time-gated microscopic imaging and spectroscopy in medical diagnosis and photobiology [also Erratum 33(11)3828(Nov1994)]

1994 • 28 citations

Delayed observation of laser-induced fluorescence for imaging of tumors

1993 • 26 citations

Fluorescence lifetime imaging of free calcium in single cells

1994 • 26 citations

<title>Frequency domain time-resolved microscope using a fast-scan CCD camera</title>

1994 • 25 citations

Double‐pulse fluorescence lifetime measurements

1997 • 24 citations

Construction and characterization of a frequency-domain fluorescence lifetime imaging microscopy system

1997 • 23 citations

Frequency-domain fluorescence lifetime imaging for endoscopic clinical cancer photodetection: Apparatus design and preliminary results

1997 • 23 citations

High quality fluorescence decay curves and lifetime imaging using an elliptical scan streak camera

1990 • 21 citations

<title>Lifetime-resolved fluorescence imaging</title>

1994 • 19 citations

Parallel acquisition of fluorescence decay using array detectors

1990 • 18 citations

Synthesis and Spectroscopic Characterization of 1-Bromo-(4-bromoacetyl)naphthalene. A Thiol-Reactive Phosphorescent Probe

1994 • 12 citations

<title>Fluorescence lifetime imaging of Ca<formula><sup><roman>2+</roman></sup></formula> using visible wavelength excitation and emission</title>

1992 • 11 citations

Time-resolved fluorescence spectroscopy. Applications to calmodulin

1991 • 10 citations

New approaches to lifetime-resolved luminescence imaging

1997 • 10 citations

Frequency-domain lifetime imaging methods at unilever research

1997 • 10 citations

<title>Confocal fluorescence lifetime imaging of pH in single cells</title>

1994 • 5 citations

<title>Time-gated fluorescence spectroscopy and imaging of porphyrins and phthalocyanines</title>

1991 • 5 citations

<title>Time-resolved ratio method for multicomponent fluorescence pattern analysis using a fluorescence lifetime imaging system</title>

1992 • 3 citations