Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Detection of specific sequences among DNA fragments separated by gel electrophoresis

1975 • 33,059 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

1979 • 22,482 citations

[57] Sequencing end-labeled DNA with base-specific chemical cleavages

1980 • 15,302 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

A comprehensive set of sequence analysis programs for the VAX

1984 • 14,824 citations

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

1977 • 12,015 citations

THE PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY

1963 • 10,322 citations

Signal transduction by receptors with tyrosine kinase activity

1990 • 5,528 citations

The Protein Kinase Family: Conserved Features and Deduced Phylogeny of the Catalytic Domains

1988 • 5,115 citations

Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing

1984 • 4,231 citations

Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs

1984 • 3,334 citations

The P-loop — a common motif in ATP- and GTP-binding proteins

1990 • 2,029 citations

Nucleotide sequence of cloned cDNA for bovine corticotropin-β-lipotropin precursor

1979 • 1,758 citations

A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures.

1987 • 1,703 citations

Functional messenger RNAs are produced by SP6in vitrotranscription of cloned cDNAs

1984 • 1,533 citations

Fast and sensitive multiple sequence alignments on a microcomputer

1989 • 1,245 citations

Type I phosphatidylinositol kinase makes a novel inositol phospholipid, phosphatidylinositol-3-phosphate

1988 • 1,027 citations

Phospholipase C-γ is a substrate for the PDGF and EGF receptor protein-tyrosine kinases in vivo and in vitro

1989 • 978 citations

PDGF-dependent tyrosine phosphorylation stimulates production of novel polyphosphoinositides in intact cells

1989 • 933 citations

Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin

1982 • 928 citations

Structural organization of the bcr gene and its role in the Ph′ translocation

1985 • 920 citations

Association of phosphatidylinositol kinase activity with polyoma middle-T competent for transformation

1985 • 813 citations

A novel viral oncogene with structural similarity to phospholipase C

1988 • 789 citations

EGF induces tyrosine phosphorylation of phospholipase C-II: A potential mechanism for EGF receptor signaling

1989 • 784 citations

DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.

1988 • 775 citations

Common elements in growth factor stimulation and oncogenic transformation: 85 kd phosphoprotein and phosphatidylinositol kinase activity

1987 • 670 citations

Cloning of bovine GAP and its interaction with oncogenic ras p21

1988 • 666 citations

Association between the PDGF receptor and members of the src family of tyrosine kinases

1990 • 650 citations

Purification and characterization of phosphoinositide 3-kinase from rat liver.

1990 • 640 citations

Binding of SH2 Domains of Phospholipase Cγ1, GAP, and Src to Activated Growth Factor Receptors

1990 • 608 citations

Structure and sequence of the cellular gene homologous to the RSV src gene and the mechanism for generating the transforming virus

1983 • 586 citations

PDGF β-receptor stimulates tyrosine phosphorylation of GAP and association of GAP with a signaling complex

1990 • 561 citations

A lymphocyte-specific protein-tyrosine kinase gene is rearranged and overexpressed in the murine T cell lymphoma LSTRA

1985 • 550 citations

Sequence similarity of phospholipase C with the non-catalytic region of src

1988 • 541 citations

Activation of phosphatidylinositol 3-kinase by insulin.

1990 • 531 citations

Autophosphorylation of the PDGF receptor in the kinase insert region regulates interactions with cell proteins

1989 • 524 citations

Role of Phosphatidylinositol Kinase in PDGF Receptor Signal Transduction

1989 • 516 citations

vav, a novel human oncogene derived from a locus ubiquitously expressed in hematopoietic cells.

1989 • 514 citations

Direct activation of the serine/threonine kinase activity of raf-1 through tyrosine phosphorylation by the PDGF β-receptor

1989 • 513 citations

Src homology region 2 domains direct protein-protein interactions in signal transduction.

1990 • 498 citations

The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway

1987 • 491 citations

Alternative splicing of RNAs transcribed from the human abl gene and from the bcr-abl fused gene

1986 • 489 citations

A Noncatalytic Domain Conserved among Cytoplasmic Protein-Tyrosine Kinases Modifies the Kinase Function and Transforming Activity of Fujinami Sarcoma Virus P130^gag-fps

1986 • 478 citations

PDGF induction of tyrosine phosphorylation of GTPase activating protein

1989 • 478 citations

Evidence that the Rous sarcoma virus transforming gene product phosphorylates phosphatidylinositol and diacylglycerol.

1984 • 476 citations

Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein.

1987 • 439 citations

Binding of Transforming Protein, P47 ^gag-crk , to a Broad Range of Phosphotyrosine-containing Proteins

1990 • 433 citations

Binding of GAP to Activated PDGF Receptors

1990 • 396 citations

Cloning of a 67-kD Neutrophil Oxidase Factor with Similarity to a Noncatalytic Region of p60 ^c-src

1990 • 393 citations

The Phosphorylation State of Eucaryotic Initiation Factor 2 Alters Translational Efficiency of Specific mRNAs

1989 • 393 citations

An 81 kd protein complexed with middle T antigen and pp60c-src: A possible phosphatidylinositol kinase

1987 • 346 citations

The colony stimulating factor-1 receptor associates with and activates phosphatidylinositol-3 kinase

1989 • 342 citations

A monoclonal antibody to the human epidermal growth factor receptor

1982 • 342 citations

Recombinant 47-Kilodalton Cytosol Factor Restores NADPH Oxidase in Chronic Granulomatous Disease

1989 • 341 citations

cDNA cloning and expression of a human platelet-derived growth factor (PDGF) receptor specific for B-chain-containing PDGF molecules.

1988 • 338 citations

Antiphosphotyrosine Recovery of Phospholipase C Activity After EGF Treatment of A-431 Cells

1988 • 324 citations

A noncatalytic domain conserved among cytoplasmic protein-tyrosine kinases modifies the kinase function and transforming activity of Fujinami sarcoma virus P130gag-fps.

1986 • 317 citations

Homology of a yeast actin-binding protein to signal transduction proteins and myosin-I

1990 • 315 citations

Evidence for two distinct phosphatidylinositol kinases in fibroblasts. Implications for cellular regulation

1987 • 307 citations

Rapid isolation of plasma membranes in high yield from cultured fibroblasts

1977 • 304 citations

Identification of a human gene (HCK) that encodes a protein-tyrosine kinase and is expressed in hemopoietic cells.

1987 • 278 citations

Transforming protein of avian sarcoma virus UR2 is associated with phosphatidylinositol kinase activity: possible role in tumorigenesis.

1984 • 278 citations

Phosphatidylinositol kinase or an associated protein is a substrate for the insulin receptor tyrosine kinase.

1990 • 266 citations

Non-catalytic domains of cytoplasmic protein-tyrosine kinases: regulatory elements in signal transduction.

1988 • 250 citations

Recent insights in phosphatidylinositol signaling

1990 • 245 citations

Phosphorylation of the PDGF receptor beta subunit creates a tight binding site for phosphatidylinositol 3 kinase.

1990 • 244 citations

Polyphosphoinositides Produced by Phosphatidylinositol 3-Kinase Are Poor Substrates for Phospholipases C from Rat Liver and Bovine Brain

1989 • 238 citations

The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck.

1987 • 236 citations

Platelet-derived growth factor (PDGF) binding promotes physical association of PDGF receptor with phospholipase C.

1989 • 235 citations

Specific Expression of a Tyrosine Kinase Gene, blk , in B Lymphoid Cells

1990 • 234 citations

Inositol phospholipid-specific phospholipase C: complete cDNA and protein sequences and sequence homology to tyrosine kinase-related oncogene products.

1988 • 223 citations

Nck, a melanoma cDNA encoding a cytoplasmic protein consisting of the src homology units SH2 and SH3

1990 • 209 citations

Phosphatidylinositol metabolism and polyoma-mediated transformation.

1986 • 203 citations

The complex of polyoma virus middle-T antigen and pp60c-src.

1984 • 196 citations

A PDGF receptor domain essential for mitogenesis but not for many other responses to PDGF

1988 • 196 citations

Association of the v-crk oncogene product with phosphotyrosine-containing proteins and protein kinase activity.

1990 • 191 citations

Tyrosine kinase activity is essential for the association of phospholipase C-gamma with the epidermal growth factor receptor.

1990 • 188 citations

Activated type I phosphatidylinositol kinase is associated with the epidermal growth factor (EGF) receptor following EGF stimulation.

1990 • 185 citations

Phosphorylation of middle T by pp60c-src: A switch for binding of phosphatidylinositol 3-kinase and optimal tumorigenesis

1989 • 178 citations

Isolation of the human insulin-like growth factor I gene using a single synthetic DNA probe.

1984 • 177 citations

B-type receptor for platelet-derived growth factor mediates a chemotactic response by means of ligand-induced activation of the receptor protein-tyrosine kinase.

1990 • 169 citations

Interactions of phosphatidylinositol kinase, GTPase-activating protein (GAP), and GAP-associated proteins with the colony-stimulating factor 1 receptor.

1990 • 167 citations

Protein kinase C mediates platelet-derived growth factor-induced tyrosine phosphorylation of p42.

1988 • 166 citations

Primary structure of the brain alpha-spectrin [published erratum appears in J Cell Biol 1989 Mar;108(3):following 1175]

1989 • 164 citations

The phosphorylation state of eucaryotic initiation factor 2 alters translational efficiency of specific mRNAs.

1989 • 163 citations

Isolation and characterization of a novel human gene expressed specifically in the cells of hematopoietic lineage.

1989 • 149 citations

Characterization of cDNA clones for the human c-yes gene.

1987 • 147 citations

Structural features of the colony-stimulating factor 1 receptor that affect its association with phosphatidylinositol 3-kinase.

1990 • 146 citations

Homologous activators of ras in fission and budding yeast

1990 • 142 citations

Molecular Cloning and Expression of a Complementary DNA for Inositol 1,4,5-Trisphosphate 3-Kinase

1990 • 142 citations

Phosphatidylinositol 3-Phosphate Is Present in Normal and Transformed Fibroblasts and Is Resistant to Hydrolysis by Bovine Brain Phospholipase C II

1989 • 137 citations

Purification and characterization of bovine brain type I phosphatidylinositol kinase

1990 • 129 citations

Bovine brain contains two types of phosphatidylinositol kinase

1987 • 125 citations

The heavy chain of Acanthamoeba myosin IB is a fusion of myosin-like and non-myosin-like sequences.

1987 • 125 citations

A computer program to enter DNA gel reading data into a computer

1984 • 123 citations

Phosphatidylinositol 3-Kinase and Its Novel Product, Phosphatidylinositol 3-phosphate, Are Present in Saccharomyces cerevisiae

1989 • 118 citations

Isolation and Oncogenic Potential of a Novel Human src-Like Gene

1986 • 113 citations

cDNA sequence for human bcr, the gene that translocates to the abl oncogene in chronic myeloid leukaemia.

1987 • 107 citations

Isolation and sequence analysis of a novel human tyrosine kinase gene.

1989 • 104 citations

Metabolic and structural evidence for the existence of a third species of polyphosphoinositide in cells: <scp>d</scp>-phosphatidyl-myo-inositol 3-phosphate

1989 • 104 citations

Phosphatidylinositol kinase type I activity associates with various oncogene products.

1989 • 98 citations

Characterization of pp85, a target of oncogenes and growth factor receptors.

1990 • 77 citations

Tyrosine phosphorylation is a signal for the trafficking of pp85, an 85-kDa phosphorylated polypeptide associated with phosphatidylinositol kinase activity.

1990 • 77 citations

Isolation and oncogenic potential of a novel human src-like gene.

1986 • 69 citations

Primary structure of the human fgr proto-oncogene product p55c-fgr.

1988 • 67 citations

Separation and characterization of a phosphatidylinositol kinase activity that co-purifies with the epidermal growth factor receptor.

1985 • 61 citations

Phosphatidylinositol turnover and transformation of cells by Abelson murine leukaemia virus.

1985 • 61 citations

Regulation of pp60c-src and its interaction with polyomavirus middle T antigen in insect cells

1990 • 59 citations

Phosphatidylinositol kinase activities in normal and Rous sarcoma virus-transformed cells.

1985 • 53 citations

Phosphatidylinositol metabolism in cells transformed by polyomavirus middle T antigen

1990 • 50 citations

Evidence from two transformed cell lines that the phosphorylations of peptide tyrosine and phosphatidylinositol are catalyzed by different proteins.

1985 • 39 citations

Phosphatidylinositol Kinase Activity in Virus-Transformed and Nontransformed Cells

1985 • 32 citations

HOMED: a homologous sequence editor

1988 • 27 citations

Phosphatidylinositol kinase activity in virus-transformed and nontransformed cells.

1985 • 25 citations

HOMED: a homologous sequence editor

1987 • 23 citations

Expression of biologically active middle T antigen of polyoma virus from recombinant baculoviruses

1989 • 12 citations