Protective vaccination with promastigote surface antigen 2 from Leishmania major is mediated by a TH1 type of immune response

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity

1981 • 4,191 citations

Phase separation of integral membrane proteins in Triton X-114 solution.

1981 • 3,366 citations

Interferon-γ and B Cell Stimulatory Factor-1 Reciprocally Regulate Ig Isotype Production

1987 • 1,936 citations

Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: Results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes

1980 • 1,256 citations

Immunoregulation of cutaneous leishmaniasis. T cell lines that transfer protective immunity or exacerbation belong to different T helper subsets and respond to distinct parasite antigens.

1988 • 713 citations

A limiting dilution assay for quantifying Leishmania major in tissues of infected mice

1985 • 508 citations

Expression Cloning of a Protective Leishmania Antigen

1995 • 351 citations

Gene replacement in parasitic protozoa

1990 • 238 citations

Effective immunization against cutaneous leishmaniasis with defined membrane antigens reconstituted into liposomes.

1988 • 236 citations

Practical progress and new drugs for changing patterns of leishmaniasis

1993 • 223 citations

Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes.

1990 • 222 citations

MURINE CUTANEOUS LEISHMANIASIS: DISEASE PATTERNS IN INTACT AND NUDE MICE OF VARIOUS GENOTYPES AND EXAMINATION OF SOME DIFFERENCES BETWEEN NORMAL AND INFECTED MACROPHAGES

1979 • 217 citations

Vaccination against cutaneous leishmaniasis in a murine model. I. Induction of protective immunity with a soluble extract of promastigotes.

1987 • 212 citations

Isolation and characterization of infective and non-infective clones of Leishmania tropica

1983 • 151 citations

Vaccines against leishmaniasis

1995 • 147 citations

Persistence of virulent Leishmania major in murine cutaneous leishmaniasis: a possible hazard for the host

1993 • 146 citations

Immunization with Leishmania receptor for macrophages protects mice against cutaneous leishmaniasis.

1985 • 145 citations

Visceral Leishmaniasis in HIV Infection and AIDS: Clinical Features and Response to Therapy

1990 • 145 citations

Membrane glycoprotein M-2 protects against Leishmania amazonensis infection

1988 • 133 citations

Recombinant vaccinia viruses expressing GP46/M-2 protect against Leishmania infection

1993 • 112 citations

Changes in the precursor frequencies of IL-4 and IFN-gamma secreting CD4+ cells correlate with resolution of lesions in murine cutaneous leishmaniasis.

1992 • 104 citations

Pure protein from Leishmania donovani protects mice against both cutaneous and visceral leishmaniasis.

1993 • 103 citations

The present and future of vaccination for cutaneous leishmaniasis.

1980 • 101 citations

Immunoprotective Leishmania major synthetic T cell epitopes.

1990 • 100 citations

Antigen-driven long term-cultured T cells proliferate in vivo, distribute widely, mediate specific tumor therapy, and persist long-term as functional memory T cells.

1986 • 97 citations

Vaccination against cutaneous leishmaniasis in a murine model. II. Immunologic properties of protective and nonprotective subfractions of soluble promastigote extract.

1987 • 97 citations

Frequency analysis of lymphokine-secreting CD4+ and CD8+ T cells activated in a graft-versus-host reaction.

1990 • 90 citations

Protection against Leishmania major infection in genetically susceptible BALB/c mice by gp63 delivered orally in attenuated Salmonella typhimurium (AroA- AroD-).

1995 • 85 citations

Cytokines in Leishmaniasis: A Complex Network of Stimulatory and Inhibitory Interactions

1993 • 77 citations

Interleukin-4 but not gamma interferon production correlates with the severity of murine cutaneous leishmaniasis

1993 • 75 citations

Molecular cloning and characterization of the immunologically protective surface glycoprotein GP46/M-2 of Leishmania amazonensis.

1990 • 68 citations

Characterization of two proteins from Leishmania donovani and their use for vaccination against visceral leishmaniasis.

1990 • 64 citations

Variants of a Leishmania surface antigen derived from a multigenic family.

1991 • 63 citations

Structural and antigenic characterization of a species- and promastigote-specific Leishmania mexicana amazonensis membrane protein.

1987 • 56 citations

Loss of the GP46/M-2 surface membrane glycoprotein gene family in the Leishmania braziliensis complex

1992 • 53 citations

Identification and characterization of host-protective T-cell epitopes of a major surface glycoprotein (gp63) from Leishmania major.

1991 • 47 citations

Development of vaccines against leishmaniasis.

1990 • 45 citations

The PSA-2 glycoprotein complex of Leishmania major is a glycosylphosphatidylinositol-linked promastigote surface antigen.

1989 • 45 citations

Characterization of a polymorphic family of integral membrane proteins in promastigotes of different Leishmania species

1994 • 35 citations

Examination of variables in the vaccination of mice against cutaneous leishmaniasis using living avirulent cloned lines and killed promastigotes of Leishmania major

1985 • 29 citations

An overview of the problem of toxoplasmosis and pneumocystosis in AIDS in the USA: Implication for future therapeutic trials

1991 • 23 citations

Resistance of BALB/c mice to Leishmania major infection is associated with a decrease in the precursor frequency of antigen-specific CD4⁺ cells secreting interleukin-4

1993 • 17 citations

Limiting dilution analysis reveals the precursors of interleukin-4-producing CD4+ cells induced by protein immunization.

1994 • 16 citations

Parasitic diseases

1990 • 13 citations

Immunity to Reinfection Following Recovery from Cutaneous Leishmaniasis (Oriental Sore).*

1955 • 13 citations

A model for the mechanism of sensitivity of BALB/c mice to L. major and premunition in leishmaniases

1987 • 10 citations