Analysis of the Yeast Transcription Factor TFIIA: Distinct Functional Regions and a Polymerase II-Specific Role in Basal and Activated Transcription

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

[19] Rapid and efficient site-specific mutagenesis without phenotypic selection

1987 • 5,413 citations

Methods in Yeast Genetics: A Laboratory Course Manual

1990 • 1,922 citations

High-Resolution Epitope Mapping of hGH-Receptor Interactions by Alanine-Scanning Mutagenesis

1989 • 1,357 citations

Methods in Yeast Genetics — A Laboratory Course Manual

1991 • 1,257 citations

Crystal structure of a yeast TBP/TATA-box complex

1993 • 1,082 citations

Co-crystal structure of TBP recognizing the minor groove of a TATA element

1993 • 1,039 citations

Five intermediate complexes in transcription initiation by RNA polymerase II

1989 • 1,033 citations

A workbench for multiple alignment construction and analysis

1991 • 1,005 citations

Functional dissection of a eukaryotic transcriptional activator protein, GCN4 of Yeast

1986 • 946 citations

Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay.

1985 • 575 citations

Assembly and function of a TCR alpha enhancer complex is dependent on LEF-1-induced DNA bending and multiple protein-protein interactions.

1995 • 546 citations

A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor.

1986 • 412 citations

The TATA-binding protein is required for transcription by all three nuclear RNA polymerases in yeast cells

1992 • 392 citations

Initiation of Transcription by RNA Polymerase II: A Multi-step Process

1993 • 390 citations

DNA topoisomerase I is involved in both repression and activation of transcription

1993 • 380 citations

Dr1, a TATA-binding protein-associated phosphoprotein and inhibitor of class II gene transcription

1992 • 379 citations

Factors involved in specific transcription in mammalian RNA polymerase II. Functional analysis of initiation factors IIA and IID and identification of a new factor operating at sequences downstream of the initiation site.

1987 • 355 citations

Each of three "TATA elements" specifies a subset of the transcription initiation sites at the CYC-1 promoter of Saccharomyces cerevisiae.

1985 • 342 citations

Rational scanning mutagenesis of a protein kinase identifies functional regions involved in catalysis and substrate interactions

1991 • 334 citations

Family of proteins that interact with TFIID and regulate promoter activity

1991 • 319 citations

Systematic mutational analysis of the yeast ACT1 gene.

1992 • 319 citations

Activation of class II gene transcription by regulatory factors is potentiated by a novel activity

1991 • 318 citations

Mot1, a global repressor of RNA polymerase II transcription, inhibits TBP binding to DNA by an ATP-dependent mechanism.

1994 • 294 citations

A systematic mutational analysis of hormone-binding determinants in the human growth hormone receptor.

1991 • 286 citations

Isolation of the gene encoding the yeast TATA binding protein TFIID: A gene identical to the SPT15 suppressor of Ty element insertions

1989 • 256 citations

Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex.

1990 • 255 citations

Variants of the TATA-binding protein can distinguish subsets of RNA polymerase I, II, and III promoters

1992 • 228 citations

A mechanism for TAFs in transcriptional activation: activation domain enhancement of TFIID-TFIIA--promoter DNA complex formation.

1994 • 207 citations

High resolution analysis of functional determinants on human tissue-type plasminogen activator.

1991 • 189 citations

The acidic activator GAL4-AH can stimulate polymerase II transcription by promoting assembly of a closed complex requiring TFIID and TFIIA.

1992 • 160 citations

An ATP-dependent inhibitor of TBP binding to DNA.

1993 • 156 citations

Transcription of a yeast U6 snRNA gene requires a polymerase III promoter element in a novel position.

1990 • 150 citations

Distinguishing between mechanisms of eukaryotic transcriptional activation with bacteriophage T7 RNA polymerase

1987 • 141 citations

Isolation of Two Genes That Encode Subunits of the Yeast Transcription Factor IIA

1992 • 137 citations

Factors Involved in Specific Transcription by Mammalian RNA Polymerase II: Purification and Analysis of Transcription Factor ILA and Identification of Transcription Factor IIJ

1992 • 136 citations

Reconstitution of transcription with five purified initiation factors and RNA polymerase II from Saccharomyces cerevisiae

1992 • 121 citations

The yeast general transcription factor TFIIA is composed of two polypeptide subunits.

1991 • 110 citations

Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex.

1993 • 110 citations

A single cDNA, hTFIIA/alpha, encodes both the p35 and p19 subunits of human TFIIA.

1993 • 108 citations

Primary sequence of the glucanase gene from Oerskovia xanthineolytica. Expression and purification of the enzyme from Escherichia coli.

1991 • 105 citations

xUBF and Rib 1 are both required for formation of a stable polymerase I promoter complex in X. laevis.

1991 • 101 citations

A multisubunit transcription factor essential for accurate initiation by RNA polymerase II

1989 • 97 citations

Transcription Factor IID Mutants Defective for Interaction with Transcription Factor IIA

1992 • 95 citations

Fractionation of the general RNA polymerase II transcription factors from Drosophila embryos.

1990 • 90 citations

Isolation of a cDNA encoding the largest subunit of TFIIA reveals functions important for activated transcription.

1993 • 88 citations

Identification of a yeast protein homologous in function to the mammalian general transcription factor, TFIIA.

1989 • 79 citations

The ZEBRA activation domain: modular organization and mechanism of action.

1993 • 62 citations

Conserved and nonconserved functions of the yeast and human TATA-binding proteins.

1994 • 44 citations

TFIIA is required for in vitro transcription of mammalian U6 genes by RNA polymerase III.

1992 • 43 citations

Affinity purification of transcription factor IIA from HeLa cell nuclear extracts.

1991 • 43 citations

Transcription factor IIA stimulates the expression of classical pollll-genes

1993 • 19 citations