The yeast exosome and human PM-Scl are related complexes of 3' right-arrow 5' exonucleases

Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases

1966 • 2,239 citations

Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry

1996 • 1,687 citations

A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides

1988 • 1,504 citations

Linking genome and proteome by mass spectrometry: Large-scale identification of yeast proteins from two dimensional gels

1996 • 1,430 citations

70K heat shock related proteins stimulate protein translocation into microsomes

1988 • 1,276 citations

A simple and efficient method for direct gene deletion inSaccharomyces cerevisiae

1993 • 1,256 citations

[36] Eukaryotic gene transcription with purified components

1983 • 992 citations

The Exosome: A Conserved Eukaryotic RNA Processing Complex Containing Multiple 3′→5′ Exoribonucleases

1997 • 939 citations

The 3' to 5' degradation of yeast mRNAs is a general mechanism for mRNA turnover that requires the SKI2 DEVH box protein and 3' to 5' exonucleases of the exosome complex

1998 • 632 citations

A DEAD-box RNA helicase in the Escherichia coli RNA degradosome

1996 • 547 citations

A small-scale procedure for preparation of nuclear extracts that support efficient transcription and pre-mRNA splicing

1988 • 485 citations

Rapid ‘de novo’ peptide sequencing by a combination of nanoelectrospray, isotopic labeling and a quadrupole/time-of-flight mass spectrometer

1997 • 461 citations

Copurification of E. coli RNAase E and PNPase: Evidence for a specific association between two enzymes important in RNA processing and degradation

1994 • 430 citations

Ribonuclease E is a 5′-end-dependent endonuclease

1998 • 411 citations

The Solution Structure of the S1 RNA Binding Domain: A Member of an Ancient Nucleic Acid–Binding Fold

1997 • 407 citations

A yeast small nuclear RNA is required for normal processing of pre-ribosomal RNA.

1987 • 313 citations

Dob1p (Mtr4p) is a putative ATP-dependent RNA helicase required for the 3' end formation of 5.8S rRNA in Saccharomyces cerevisiae

1998 • 303 citations

Delayed Extraction Improves Specificity in Database Searches by Matrix-assisted Laser Desorption/Ionization Peptide Maps

1996 • 297 citations

Ribonuclease E organizes the protein interactions in the Escherichia coli RNA degradosome

1998 • 297 citations

Rrp6p, the Yeast Homologue of the Human PM-Scl 100-kDa Autoantigen, Is Essential for Efficient 5.8 S rRNA 3′ End Formation

1998 • 294 citations

Identification and functional analysis of two U3 binding sites on yeast pre-ribosomal RNA.

1992 • 257 citations

Identification of the Components of Simple Protein Mixtures by High-Accuracy Peptide Mass Mapping and Database Searching

1997 • 229 citations

The 3' end of yeast 5.8S rRNA is generated by an exonuclease processing mechanism.

1996 • 208 citations

Importin Provides a Link between Nuclear Protein Import and U snRNA Export

1996 • 189 citations

Purification of NSP1 reveals complex formation with ‘GLFG’ nucleoporins and a novel nuclear pore protein NIC96.

1993 • 189 citations

Comparative sequence analysis of ribonucleases HII, III, II PH and D

1997 • 183 citations

The vacB Gene Required for Virulence inShigella flexneri and Escherichia coli Encodes the Exoribonuclease RNase R

1998 • 163 citations

Peptide Sequencing by Mass Spectrometry for Homology Searches and Cloning of Genes

1997 • 125 citations

Immunolocalization and partial characterization of a nucleolar autoantigen (PM-Scl) associated with polymyositis/scleroderma overlap syndromes.

1986 • 124 citations

Molecular characterization of an autoantigen of PM-Scl in the polymyositis/scleroderma overlap syndrome: a unique and complete human cDNA encoding an apparent 75-kD acidic protein of the nucleolar complex.

1991 • 119 citations

A U3 snoRNP protein with homology to splicing factor PRP4 and G beta domains is required for ribosomal RNA processing.

1993 • 118 citations

Synthetic lethality with fibrillarin identifies NOP77p, a nucleolar protein required for pre-rRNA processing and modification.

1994 • 107 citations

A DEAD-Box-Family Protein Is Required for Nucleocytoplasmic Transport of Yeast mRNA

1996 • 101 citations

Nip7p Interacts with Nop8p, an Essential Nucleolar Protein Required for 60S Ribosome Biogenesis, and the Exosome Subunit Rrp43p

1999 • 99 citations

One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins

1996 • 98 citations

hPop1: an autoantigenic protein subunit shared by the human RNase P and RNase MRP ribonucleoproteins.

1996 • 95 citations

Identifying proteins and post-translational modifications by mass spectrometry

1998 • 94 citations

Cloning and characterization of the cDNA coding for a polymyositis-scleroderma overlap syndrome-related nucleolar 100-kD protein.

1992 • 88 citations

Cloning of a complementary DNA coding for the 100-kD antigenic protein of the PM-Scl autoantigen.

1992 • 76 citations

Identification of protein components reactive with anti-PM/Scl autoantibodies

1990 • 65 citations

Human Dis3p, Which Binds to Either GTP- or GDP-Ran, Complements Saccharomyces cerevisiae dis3

1998 • 58 citations

Mutations in nucleolar proteins lead to nucleolar accumulation of polyA+ RNA in Saccharomyces cerevisiae.

1995 • 54 citations

Mutations Synthetically Lethal with cep1 Target S. cerevisiae Kinetochore Components

1998 • 45 citations

Autoantibodies Against Nuclear, Nucleolar, and Mitochondrial Antigens in Systemic Sclerosis (Scleroderma)

1990 • 44 citations

Delayed Extraction Improves Specificity in Database Searches by Matrix‐assisted Laser Desorption/Ionization Peptide Maps

1996 • 11 citations