Purification of a Protein Palmitoyltransferase that Acts on H-Ras Protein and on a C-terminal N-Ras Peptide

All ras proteins are polyisoprenylated but only some are palmitoylated

1989 • 1,806 citations

A polybasic domain or palmitoylation is required in addition to the CAAX motif to localize p21ras to the plasma membrane

1990 • 1,049 citations

MAP kinase pathways in yeast: For mating and more

1995 • 1,009 citations

p21ras is modified by a farnesyl isoprenoid.

1989 • 847 citations

Inhibition of purified p21ras farnesyl:protein transferase by Cys-AAX tetrapeptides

1990 • 815 citations

THE BIOLOGY AND ENZYMOLOGY OF EUKARYOTIC PROTEIN ACYLATION

1988 • 787 citations

The p21 ras C-terminus is required for transformation and membrane association

1984 • 590 citations

Post-translational processing of p21ras is two-step and involves carboxyl-methylation and carboxy-terminal proteolysis.

1989 • 462 citations

Localization of the src gene product of the Harvey strain of MSV to plasma membrane of transformed cells by electron microscopic immunocytochemistry

1980 • 404 citations

Posttranslational modification of the Ha-ras oncogene protein: evidence for a third class of protein carboxyl methyltransferases.

1988 • 384 citations

Structure of Saccharomyces cerevisiae mating hormone a-factor. Identification of S-farnesyl cysteine as a structural component.

1988 • 373 citations

The transforming proteins of Rous sarcoma virus, Harvey sarcoma virus and Abelson virus contain tightly bound lipid

1982 • 357 citations

Purification and properties of a palmitoyl-protein thioesterase that cleaves palmitate from H-Ras.

1993 • 344 citations

Human Lamin B Contains a Farnesylated Cysteine Residue

1989 • 324 citations

Dynamic fatty acylation of p21N-ras.

1987 • 309 citations

Improvement and simplification of low‐background silver staining of proteins by using sodium dithionite

1988 • 297 citations

Methylation and proteolysis are essential for efficient membrane binding of prenylated p21K-ras(B).

1991 • 291 citations

Molecular cloning and expression of palmitoyl-protein thioesterase.

1994 • 229 citations

Enzymatic Coupling of Cholesterol Intermediates to a Mating Pheromone Precursor and to the Ras Protein

1990 • 175 citations

Identification of a precursor in the biosynthesis of the p21 transforming protein of harvey murine sarcoma virus

1982 • 151 citations

Biochemical Characterization of a Palmitoyl Acyltransferase Activity That Palmitoylates Myristoylated Proteins

1995 • 147 citations

Structure of rhodotorucine A, a novel lipopeptide, inducing mating tube formation in Rhodosporidiumtoruloides

1978 • 145 citations

Posttranslational processing of p21 ras proteins involves palmitylation of the C-terminal tetrapeptide containing cysteine-186

1985 • 120 citations

Binding of prenylated and polybasic peptides to membranes: affinities and intervesicle exchange

1995 • 113 citations

Processing and fatty acid acylation of RAS1 and RAS2 proteins in Saccharomyces cerevisiae.

1986 • 108 citations

Palmitoylation of Ha-Ras Facilitates Membrane Binding, Activation of Downstream Effectors, and Meiotic Maturation in Xenopus Oocytes

1996 • 99 citations

Fatty acylation is important but not essential for Saccharomyces cerevisiae RAS function.

1987 • 98 citations

Ras membrane targeting is essential for glucose signaling but not for viability in yeast.

1995 • 98 citations

Cell-free fatty acid acylation of Semliki Forest viral polypeptides with microsomal membranes from eukaryotic cells.

1984 • 89 citations

Protein Modification: Palmitoylation in G-protein signaling pathways

1995 • 78 citations

Characterization of an acyltransferase acting on p21N-ras protein in a cell-free system

1991 • 69 citations

Cysteine-Containing Peptide Sequences Exhibit Facile Uncatalyzed Transacylation and Acyl-CoA-dependent Acylation at the Lipid Bilayer Interface

1994 • 69 citations

The Farnesyl Group of H-Ras Facilitates the Activation of a Soluble Upstream Activator of Mitogen-activated Protein Kinase

1995 • 60 citations

Synergistic activation by Ras and 14-3-3 protein of a mitogen-activated protein kinase kinase kinase named Ras-dependent extracellular signal-regulated kinase kinase stimulator.

1994 • 55 citations

Myelin P₀ Glycoprotein and a Synthetic Peptide Containing the Palmitoylation Site Are Both Autoacylated

1995 • 50 citations

A microsomal endoprotease that specifically cleaves isoprenylated peptides.

1992 • 48 citations

A prenylated protein-specific endoprotease in rat liver microsomes that produces a carboxyl-terminal tripeptide

1993 • 32 citations

[2] Purification of baculovirus-expressed recombinant ras and rap proteins

1995 • 29 citations

[9] Preparation of radioactive acyl coenzyme A

1986 • 22 citations

Purification and characterisation of the protein encoded by the activated human N-ras gene and its membrane localisation.

1987 • 20 citations

[16] Synthetic prenylated peptides: Studying prenyl protein-specific endoprotease and other aspects of protein prenylation

1995 • 17 citations

Solubilization of protein fatty acyltransferase from placental membranes and cell-free acyl transfer on to exogenous and endogenous acceptors

1989 • 12 citations