Strategies for the Genetic Manipulation ofSaccharomyces cerevisiae

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

1989 • 7,496 citations

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

1992 • 4,683 citations

Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

1977 • 3,829 citations

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction

1986 • 3,400 citations

Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis

1984 • 2,847 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

Transformation of yeast.

1978 • 2,267 citations

High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier

1989 • 2,140 citations

Efficient isolation of genes by using antibody probes.

1983 • 2,065 citations

Genetic Control of the Cell Division Cycle in Yeast

1974 • 1,588 citations

Cloning of Large Segments of Exogenous DNA into Yeast by Means of Artificial Chromosome Vectors

1987 • 1,517 citations

High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

1979 • 1,504 citations

Yeast transformation: a model system for the study of recombination.

1981 • 1,491 citations

[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

1987 • 1,375 citations

Transformation of yeast by a replicating hybrid plasmid

1978 • 1,362 citations

The molecular biology of the yeast saccharomyces: Life cycle and inheritance

1982 • 1,361 citations

A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector

1987 • 1,181 citations

Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes

1986 • 1,148 citations

The molecular biology of the yeast saccharomyces: Metabolism and gene expression

1983 • 1,118 citations

Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experiments

1979 • 967 citations

Oligonucleotide-Directed Mutagenesis: A Simple Method Using Two Oligonucleotide Primers and a Single-Stranded DNA Template

1984 • 947 citations

Ty elements transpose through an RNA intermediate

1985 • 837 citations

Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase

1988 • 814 citations

[12] High-efficiency transformation of yeast by electroporation

1991 • 798 citations

Isolation and characterisation of a yeast chromosomal replicator

1979 • 795 citations

Isolation of a yeast centromere and construction of functional small circular chromosomes

1980 • 788 citations

An electrophoretic karyotype for yeast.

1985 • 787 citations

Replacement of chromosome segments with altered DNA sequences constructed in vitro.

1979 • 779 citations

GENETIC CONTROL OF THE CELL DIVISION CYCLE IN YEAST: V. GENETIC ANALYSIS OF cdc MUTANTS

1973 • 728 citations

KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene

1989 • 684 citations

Cloning yeast telomeres on linear plasmid vectors

1982 • 625 citations

A regulatory hierarchy for cell specialization in yeast

1989 • 612 citations

Life cycle of the budding yeast Saccharomyces cerevisiae

1988 • 565 citations

The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation

1989 • 561 citations

Plasmid construction by homologous recombination in yeast

1987 • 560 citations

A mutant of Saccharomyces cerevisiae defective for nuclear fusion.

1976 • 530 citations

Sequence of a yeast DNA fragment containing a chromosomal replicator and the TRP1 gene

1980 • 511 citations

Ethidium bromide induced mutation of yeast mitochondria: Complete transformation of cells into respiratory deficient non-chromosomal “petites”

1968 • 504 citations

[14] Genetic applications of yeast transformation with linear and gapped plasmids

1983 • 503 citations

Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene.

1980 • 502 citations

Mitotic stability of yeast chromosomes: A colony color assay that measures nondisjunction and chromosome loss

1985 • 486 citations

Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae.

1987 • 476 citations

A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast

1983 • 462 citations

Construction of artificial chromosomes in yeast

1983 • 448 citations

Transformation in fungi

1989 • 443 citations

Loss of BiP/GRP78 function blocks translocation of secretory proteins in yeast.

1990 • 435 citations

Yeast recombination: the association between double-strand gap repair and crossing-over.

1983 • 432 citations

Expression of a transposable antibiotic resistance element in Saccharomyces

1980 • 428 citations

GENETICS OF MITOCHONDRIAL BIOGENESIS

1986 • 420 citations

Inhibitors of protein synthesis

1974 • 411 citations

Isolation of the β-tubulin gene from yeast and demonstration of its essential function in vivo

1983 • 409 citations

Pedigree analysis of plasmid segregation in yeast

1983 • 400 citations

Genetic map of Saccharomyces cerevisiae

1980 • 394 citations

KAR1, a gene required for function of both intranuclear and extranuclear microtubules in yeast

1987 • 372 citations

Tandem gene amplification mediates copper resistance in yeast.

1982 • 354 citations

ras-Related gene sequences identified and isolated from Saccharomyces cerevisiae

1983 • 345 citations

IN VITRO MUTAGENESIS

1985 • 338 citations

Pseudogenes in yeast?

1987 • 316 citations

Mitochondrial Transformation in Yeast by Bombardment with Microprojectiles

1988 • 314 citations

Genetic analysis of the mitotic transmission of minichromosomes

1985 • 310 citations

The HTS1 gene encodes both the cytoplasmic and mitochondrial histidine tRNA synthetases of S. cerevisiae

1986 • 308 citations

A site-specific endonuclease essential for mating-type switching in Saccharomyces cerevisiae

1983 • 302 citations

The presence of a defective LEU2 gene on 2 mu DNA recombinant plasmids of Saccharomyces cerevisiae is responsible for curing and high copy number

1983 • 300 citations

Lethal Disruption of the Yeast Actin Gene by Integrative DNA Transformation

1982 • 298 citations

Functional expression of cloned yeast DNA in Escherichia coli.

1977 • 284 citations

Yeast: an Experimental Organism for Modern Biology

1988 • 284 citations

Copper metallothionein of yeast, structure of the gene, and regulation of expression.

1984 • 283 citations

Genetic map of Saccharomyces cerevisiae, edition 10

1989 • 276 citations

The yeast cell, its genetics and cytology

1949 • 270 citations

Functional genetic expression of eukaryotic DNA in Escherichia coli.

1976 • 261 citations

THE ROLE OF RADIATION (rad) GENES IN MEIOTIC RECOMBINATION IN YEAST

1980 • 257 citations

Yeast chromosome replication and segregation

1988 • 250 citations

Life cycle of the budding yeast Saccharomyces cerevisiae.

1988 • 242 citations

Inhibitors of Protein Biosynthesis

1979 • 233 citations

Saccharomyces cerevisiae contains two functional genes encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase.

1986 • 231 citations

SELECTION OF lys2 MUTANTS OF THE YEAST SACCHAROMYCES CEREVISIAE BY THE UTILIZATION OF α-AMINOADIPATE

1979 • 231 citations

Autonomously replicating sequences in Saccharomyces cerevisiae.

1980 • 222 citations

Meiotic Gene Conversion: A Signal of the Basic Recombination Event in Yeast

1979 • 221 citations

Dominant effects of tubulin overexpression in Saccharomyces cerevisiae.

1989 • 191 citations

An Enrichment Method for Auxotrophic Yeast Mutants using the Antibiotic ‘Nystatin’

1966 • 189 citations

Identification of ten genes that control ribosome formation in yeast

1970 • 182 citations

High Efficiency Transformation of Intact Yeast Cells by Electric Field Pulses

1990 • 178 citations

Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene.

1984 • 177 citations

Physical mapping of large DNA by chromosome fragmentation.

1988 • 170 citations

Yeast plasmid requires a cis-acting locus and two plasmid proteins for its stable maintenance

1983 • 167 citations

Plasmids pEMBLY: new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences

1985 • 157 citations

Fusion of yeast spheroplasts

1977 • 145 citations

Chromosome length controls mitotic chromosome segregation in yeast

1986 • 144 citations

Directed Deletion of a Yeast Transfer RNA Intervening Sequence

1980 • 142 citations

A novel method for transformation of intact yeast cells by electroinjection of plasmid DNA

1985 • 141 citations

CHROMOSOMAL TRANSLOCATIONS GENERATED BY HIGH-FREQUENCY MEIOTIC RECOMBINATION BETWEEN REPEATED YEAST GENES

1986 • 138 citations

Control of Cell Type in Saccharomyces cerevisiae: Mating Type and Mating-type Interconversion

1981 • 132 citations

Internuclear transfer of genetic information in kar1-1/KAR1 heterokaryons in Saccharomyces cerevisiae.

1981 • 130 citations

Direct selection of Saccharomyces cerevisiae resistant to the antibiotic G418 following transformation with a DNA vector carrying the kanamycin-resistance gene of Tn903

1983 • 130 citations

Plasmid Vectors Carrying the Replication Origin of Filamentous Single-Stranded Phages

1987 • 127 citations

Mutations in elongation factor EF-1 alpha affect the frequency of frameshifting and amino acid misincorporation in Saccharomyces cerevisiae.

1988 • 125 citations

Yeast — A Practical Approach

1988 • 125 citations

Genetic properties of chromosomally integrated 2μ plasmid DNA in yeast

1982 • 123 citations

Genetic differences between Saccharomyces carlsbergensis and S. cerevisiae. Analysis of chromosome III by single chromosome transfer

1981 • 121 citations

Induction by manganese of mitochondrial antibiotic resistance mutations in yeast

1973 • 121 citations

Identification and isolation of the yeast cytochrome c gene

1978 • 119 citations

A General Method for the Chromosomal Amplification of Genes in Yeast

1988 • 118 citations

Yeast chromosome replication and segregation.

1988 • 115 citations

The yeast ARS element, six years on: A progress report

1985 • 114 citations

Yeast artificial chromosomes: tools for mapping and analysis of complex genomes

1990 • 110 citations

Fungal Protoplasts: Applications in Biochemistry and Genetics

1985 • 106 citations

Chromosome engineering in Saccharomyces cerevisiae by using a site-specific recombination system of a yeast plasmid

1990 • 106 citations

Genetic effects of methyl benzimidazole-2-yl-carbamate on Saccharomyces cerevisiae.

1982 • 105 citations

Mitotic chromosome loss in a radiation-sensitive strain of the yeast Saccharomyces cerevisiae.

1981 • 105 citations

Partial suppression of an ochre mutation in Saccharomyces cerevisiae by multicopy plasmids containing a normal yeast tRNAGln gene

1985 • 100 citations

Hygromycin B resistance as dominant selectable marker in yeast

1984 • 97 citations

Toxic Effects of Excess Cloned Centromeres

1986 • 96 citations

The transformation of brewing yeasts with a plasmid containing the gene for copper resistance

1985 • 95 citations

[53] Isolation of genes by complementation in yeast

1987 • 95 citations

Generation of deletion derivatives by targeted transformation of human-derived yeast artificial chromosomes.

1990 • 93 citations

The genetic manipulation of industrial yeast strains

1981 • 92 citations

Transformation of Saccharomyces cerevisiae spheroplasts by high electric pulse

1985 • 90 citations

A RAPID CHROMOSOME-MAPPING METHOD FOR CLONED FRAGMENTS OF YEAST DNA

1983 • 87 citations

Construction of Lactose-Assimilating and High-Ethanol-Producing Yeasts by Protoplast Fusion

1986 • 87 citations

GENETIC IMPROVEMENT OF INDUSTRIAL YEASTS

1983 • 82 citations

Phleomycin resistance encoded by the ble gene from transposon Tn 5 as a dominant selectable marker in Saccharomyces cerevisiae

1987 • 82 citations

Selection of spontaneous mutants by inositol starvation in yeast

1975 • 82 citations

Transformation of yeast by agitation with glass beads.

1988 • 81 citations

Elongation factor EF-1 alpha gene dosage alters translational fidelity in Saccharomyces cerevisiae.

1989 • 79 citations

Characterization of the transmission during cytoductant formation of the 2 μm DNA plasmid from Saccharomyces

1981 • 79 citations

[31] Directed mutagenesis with sodium bisulfite

1983 • 77 citations

Amplification of large artificial chromosomes.

1990 • 72 citations

Expression of the Herpes simplex virus thymidine kinase gene in Saccharomyces cerevisiae

1981 • 71 citations

Saccharomyces cerevisiae CYC1 mRNA 5′-End Positioning: Analysis by In Vitro Mutagenesis, Using Synthetic Duplexes with Random Mismatch Base Pairs

1985 • 71 citations

An efficient chloramphenicol-resistance marker for Saccharomyces cerevisiae and Escherichia coli

1986 • 71 citations

One-step gene replacement in yeast by cotransformation

1985 • 70 citations

Loss of 2 um DNA from Saccharomyces cerevisiae transformed with the chimaeric plasmid pJDB219

1980 • 69 citations

Transfer of chromosome III duringkar mediated cytoduction in yeast

1980 • 69 citations

Toxic effects of excess cloned centromeres.

1986 • 66 citations

Isolation and characterization of the yeast 3-phosphoglycerokinase gene (PGK) by an immunological screening technique.

1980 • 63 citations

Nitrosoguanidine mutagenesis during nuclear and mitochondrial gene replication

1974 • 62 citations

Phenotype character of the methylglyoxal resistance gene in Saccharomyces cerevisiae: expression in Escherichia coli and application to breeding wild-type yeast strains

1985 • 61 citations

Transformation of Protoplasted Yeast Cells Is Directly Associated with Cell Fusion

1984 • 59 citations

rme1 Mutation of Saccharomyces cerevisiae: map position and bypass of mating type locus control of sporulation.

1981 • 59 citations

Isolation of structural genes for yeast RNA polymerases by immunological screening.

1986 • 56 citations

Cloning in Yeast

1987 • 54 citations

[22] Cloning of yeast STE genes in 2 μm vectors

1983 • 54 citations

Transformation of protoplasted yeast cells is directly associated with cell fusion.

1984 • 51 citations

Electro-fusion of haploid Saccharomyces yeast cells of identical mating type

1983 • 48 citations

Saccharomyces cerevisiae Mutants Resistant to Catabolite Repression: Use in Cheese Whey Hydrolysate Fermentation

1982 • 46 citations

Cloning with 2-μm DNA Vectors and the Expression of Foreign Genes in Saccharomyces cerevisiae

1982 • 45 citations

VARIATION OF MUTAGENIC ACTION ON NONSENSE MUTANTS AT DIFFERENT SITES IN THE ISO-1-CYTOCHROME c GENE OF YEAST

1974 • 41 citations

A novel class of vector for yeast transformation

1989 • 40 citations

[24] Transformation of yeast directly with synthetic oligonucleotides

1991 • 38 citations

Saccharomyces cerevisiae CYC1 mRNA 5'-end positioning: analysis by in vitro mutagenesis, using synthetic duplexes with random mismatch base pairs.

1985 • 37 citations

Micro Review Bending the rules: the 2μ plasmid of yeast

1987 • 36 citations

Factors affecting high-frequency fungal protoplast fusion

1976 • 36 citations

Genetic transformation of Saccharomyces cerevisiae with single-stranded circular DNA vectors

1982 • 35 citations

Construction of stable laboratory and industrial yeast strains expressing a foreign gene by integrative transformation using a dominant selection system

1986 • 31 citations

Host-Plasmid Interactions in Saccharomyces cerevisiae: Effect of Host Ploidy on Plasmid Stability and Copy Number

1989 • 31 citations

The allosuppressor gene SAL4 encodes a protein important for maintaining translational fidelity in Saccharomyces cerevisiae

1988 • 30 citations

Genetic selection for reciprocal translocation at chosen chromosomal sites in Saccharomyces cerevisiae.

1982 • 29 citations

Oligodeoxynucleotide-Directed Mutagenesis ofEscherichia coliand Yeast by Simple Cotransformation of the Primer and Template

1986 • 29 citations

An immunological method for detecting gene expression in yeast colonies.

1984 • 26 citations

Vectors for Cloning in Yeast

1982 • 25 citations

Control of recombination within and between DNA plasmids of Saccharomyces cerevisiae

1980 • 25 citations

Oligodeoxynucleotide-directed mutagenesis using the yeast transformation system

1986 • 23 citations

Curing of endogenous 2 micron DNA in yeast by recombinant vectors

1987 • 22 citations

Retrovirus-like vectors for Saccharomyces cerevisiae: integration of foreign genes controlled by efficient promoters into yeast chromosomal DNA

1988 • 21 citations

A rapid and efficient procedure for transformation of intact Saccharomyces cerevisiae by electroporation

1989 • 19 citations

Saccharomyces

1991 • 17 citations

Morphological and genetic effects of benomyl on polyploid brewing yeasts: isolation of auxotrophic mutants

1984 • 16 citations

High-efficiency transformation of Saccharomyces cerevisiae cells by bacterial minicell protoplast fusion.

1986 • 14 citations

New vectors for construction of recombinant high-copy-number yeast acentric-ring plasmids

1985 • 14 citations

Expression of Heterologous Genes

1991 • 14 citations

Expression of proteins encoded by foreign genes in Saccharomyces cerevisiae

1989 • 13 citations

The Analysis of Temperature-Sensitive Mutants of Saccharomyces Cerevisiae Altered in Components Required for Protein Synthesis

1988 • 12 citations

Hybridization and Polyploidization of Saccharomyces cerevisiae Strains by Transformation-Associated Cell Fusion

1985 • 12 citations

Methods in Classical Genetics

1991 • 11 citations

Use of the LYS2 Gene for Gene Disruption, Gene Replacement, and Promoter Analysis in Saccharomyces cerevisiae

1985 • 10 citations

A comprehensive compilation of 400 nucleotide sequences coding for proteins from the yeast Saccharomyces cerevisiae = LISTA1

1988 • 9 citations

Use of liposomes in transforming yeast cells

1985 • 7 citations

Molecular Breeding of Yeasts for Production of Useful Compounds: Novel Methods of Transformation and New Vector Systems

1986 • 6 citations

[14] Site-directed chromosomal rearrangements in yeast

1987 • 6 citations

Classical genetic and protoplast fusion techniques in yeast

1987 • 5 citations

Oligonucleotide-directed misincorporation mutagenesis on singlestranded DNA templates

1986 • 4 citations