Classical genetic and protoplast fusion techniques in yeast

The Journal of General Microbiology

1985 • 2,446 citations

The molecular biology of the yeast saccharomyces: Life cycle and inheritance

1982 • 1,361 citations

Electric field-mediated fusion and related electrical phenomena

1982 • 1,008 citations

The Molecular biology of the yeast saccharomyces, life cycle and inheritance

1981 • 636 citations

A method for high-frequency intergeneric fusion of plant protoplasts

1974 • 623 citations

Electric field-induced cell-to-cell fusion

1982 • 580 citations

A mutant of Saccharomyces cerevisiae defective for nuclear fusion.

1976 • 530 citations

Tetrazolium Overlay Technique for Population Studies of Respiration Deficiency in Yeast

1957 • 475 citations

Expression of a transposable antibiotic resistance element in Saccharomyces

1980 • 428 citations

Tandem gene amplification mediates copper resistance in yeast.

1982 • 354 citations

JOURNAL OF THE INSTITUTE OF BREWING

1941 • 342 citations

High frequency fusion of plant protoplasts by electric fields

1981 • 267 citations

A Method of isolating Protoplasts from Yeast

1957 • 227 citations

CURRENT DEVELOPMENTS IN YEAST RESEARCH

1981 • 225 citations

ISOLATION AND PROPERTIES OF INTACT MITOCHONDRIA FROM SPHEROPLASTS OF YEAST

1964 • 218 citations

Fermentation of D-xylose to ethanol by a strain ofCandida shehatae

1983 • 210 citations

Genetics of Industrial Microorganisms

1979 • 208 citations

Genetics of industrial microorganisms

1978 • 200 citations

Protoplast fusion of Schizosaccharomyces pombe auxotrophic mutants of identical mating-type

1977 • 184 citations

Fusion of bacterial protoplasts.

1976 • 175 citations

Current topics in microbiology and immunology

1971 • 154 citations

Induced Fusion of Fungal Protoplasts following Treatment with Polyethylene Glycol

1976 • 152 citations

Mitochondria 1977, genetics and biogenesis of mitochondria

1978 • 152 citations

Cell Wall of Yeasts

1963 • 151 citations

Fusion of yeast spheroplasts

1977 • 145 citations

Fusion of protoplasts of Bacillus megaterium.

1976 • 127 citations

The Production and Selection of a Family of Strains in Penicillium Chrysogenum

1955 • 109 citations

Mechanisms of Meiotic Gene Conversion, or “Wanderings on a Foreign Strand”

1981 • 105 citations

Genetic effects of methyl benzimidazole-2-yl-carbamate on Saccharomyces cerevisiae.

1982 • 105 citations

Genetic and Biochemical Analysis of the Ability of Saccharomyces cerevisiae to Decarboxylate Cinnamic Acids

1982 • 105 citations

Hybridization of Candida albicans through fusion of protoplasts

1981 • 104 citations

ONE HUNDRED YEARS OF YEAST RESEARCH AND DEVELOPMENT IN THE BREWING INDUSTRY

1986 • 95 citations

Current Topics in Microbiology and Immunology

1981 • 95 citations

Purification and properties of an enzyme, zymolyase, which lyses viable yeast cells

1972 • 94 citations

The genetic manipulation of industrial yeast strains

1981 • 92 citations

Fermentation of <scp>D</scp>-xylose, xylitol, and <scp>D</scp>-xylulose by yeasts

1982 • 90 citations

Transformation of Saccharomyces cerevisiae spheroplasts by high electric pulse

1985 • 90 citations

Construction of Lactose-Assimilating and High-Ethanol-Producing Yeasts by Protoplast Fusion

1986 • 87 citations

An improved electrofusion technique for production of mouse hybridoma cells

1985 • 85 citations

High-frequency fusion of fungal protoplasts

1975 • 82 citations

Somatic segregation of the killer (k) and neutral (n) cytoplasmic genetic determinants in yeast

1969 • 79 citations

The Production and Selection of a Family of Strains in Penicillium chrysogenum

1955 • 69 citations

Biotechnology in food processing

1986 • 69 citations

PRODUCTION OF AMYLOLYTIC ENZYMES BY SEVERAL YEAST SPECIES

1982 • 68 citations

Electrofusion

1984 • 66 citations

Recombination after protoplast fusion in the yeast Candida tropicalis

1977 • 66 citations

FERMENTATION PROPERTIES OF BREWING YEAST WITH KILLER CHARACTER

1984 • 61 citations

Zygote formation and recombination between like mating types in the yeast Saccharomycopsis lipolytica by protoplast fusion

1978 • 60 citations

Sexual agglutination in Saccharomyces cerevisiae

1981 • 60 citations

Electrofusion of cells: principles and industrial potential

1983 • 60 citations

Electric Field‐induced Fusion of Sea Urchin Eggs

1981 • 58 citations

Genetic construction of yeast strains for high ethanol production

1983 • 54 citations

Electric field-induced fusion of large liposomes from natural and polymerizable lipids

1982 • 54 citations

Fusion of Yeast Protoplasts Induced by Polyethylene Glycol

1978 • 54 citations

Ionic modulation of electrically induced fusion of mammalian cells

1985 • 53 citations

Intergeneric hybridization in yeasts through protoplast fusion

1978 • 51 citations

Starch hydrolysis by derepressed mutants of Schwanniomyces castellii

1983 • 49 citations

Chapter XXIV Production and Use of Fungal Protoplasts

1971 • 49 citations

Electro-fusion of haploid Saccharomyces yeast cells of identical mating type

1983 • 48 citations

Saccharomyces cerevisiae Mutants Resistant to Catabolite Repression: Use in Cheese Whey Hydrolysate Fermentation

1982 • 46 citations

Regeneration of Mycelial Protoplasts of Fusarium culmorum

1966 • 45 citations

Mitotic Chromosome Loss Induced by Methyl Benzimidazole-2-yl-Carbamate as a Rapid Mapping Method in Saccharomyces cerevisiae

1982 • 45 citations

Transformation of yeast without the use of foreign DNA

1979 • 43 citations

Intergeneric hybrids of Saccharomyces cerevisiae and Zygosaccharomyces fermentati obtained by protoplast fusion

1986 • 42 citations

Large-scale production of yeast hybrids by electrofusion

1984 • 41 citations

The use of mitochondrial mutants in hybridization of industrial yeasts

1981 • 41 citations

Influence of the composition of the fusion medium on the yield of electrofused yeast hybrids

1985 • 39 citations

Cytoduction as a new tool in studying the cytoplasmic heredity in yeast

1977 • 39 citations

Protoplast fusion in the yeast, Schwanniomyces alluvius

1982 • 39 citations

A stable plasmid carrying the yeast Leu2 gene and containing only yeast deoxyribonucleic acid

1980 • 38 citations

Factors affecting high-frequency fungal protoplast fusion

1976 • 36 citations

Interspecific hybrid production between the yeastsKluyveromyces lactisandKluyveromyces fragilisby protoplast fusion

1978 • 35 citations

Synthesis of [1,2-3H2]Medroxyprogesterone Acetate (17α-Acetoxy-6α-methyl[1,2-3H2]pregn-4-ene-3,20-dione) and [1,2-3H2]Megestrol Acetate (17α-Acetoxy-6-methyl[1,2-3H2]pregna-4,6-diene-3,20-dione)

1964 • 35 citations

Mitotic chromosome loss induced by methyl benzimidazole-2-yl-carbamate as a rapid mapping method in Saccharomyces cerevisiae.

1982 • 35 citations

Electrofusion of myeloma cells on the single cell level

1983 • 34 citations

The use of mitochondrial mutants in the isolation of hybrids involving industrial yeast strains

1980 • 33 citations

ANALYSIS OF SPORULATION IN BREWER'S YEAST: INDUCTION OF TETRAD FORMATION*

1986 • 31 citations

Fusion of Rhodosporidium (Rhodotorula) protoplasts

1977 • 30 citations

Annual Review of Biochemistry

1964 • 30 citations

Transfer of mitochondrial function into a cytoplasmic respiratory-deficient mutant of Saccharomyces yeast by electro-fusion

1982 • 30 citations

Ethanol formation by hybrid yeasts

1986 • 29 citations

Protoplast fusion used for the construction of presumptive polyploids of the D-xylose fermenting yeast Candida shehatae

1985 • 28 citations

The use of mitochondrial mutants in hybridization of industrial yeast strains

1985 • 26 citations

UTILIZATION BY YEASTS OF THE CARBOHYDRATES OF WORT

1955 • 26 citations

Vectors for Cloning in Yeast

1982 • 25 citations

An enzyme which degrades the walls of living yeast

1966 • 24 citations

The use of mitochondrial mutants in hybridization of industrial yeast strains

1985 • 23 citations

Fusion of Yeast Protoplasts

1977 • 23 citations

PHYSIOLOGICAL REQUIREMENTS FOR INDUCTION OF SPORULATION IN LAGER YEAST*

1987 • 22 citations

Studies on protoplast fusion. Part I. Use of protoplast fusion for the development of rapid starch-fermenting strains of Saccharomyces diastaticus.

1986 • 20 citations

Formation of intergeneric hybrids of yeast by protoplast fusion of Yarrowia and Kluyveromyces species

1984 • 20 citations

Induction of gene conversion and reverse mutation by manganese sulphate and nickel sulphate in Saccharomyces cerevisiae

1984 • 19 citations

Potenytial cost savings for fuel ethanol production by employing a novel hybrid yeast strain

1985 • 18 citations

YEAST MUTANTS EXCRETING VITAMIN B₁AND THEIR USE IN THE PRODUCTION OF THIAMINE RICH BEERS*,†

1985 • 18 citations

Selection of yeast auxotrophs by thymidylate starvation

1977 • 18 citations

Dielectrophoretic properties of yeast cells dividing by budding and by transversal fission

1984 • 17 citations

STUDIES ON THE FORMATION OF SPHEROPLASTS FROM STATIONARY PHASE CELLS OFSACCHAROMYCES CEREVISIAE

1973 • 17 citations

Lactose-utilizing hybrid strain derived from Saccharomyces cerevisiae and Kluyveromyces lactis by protoplast fusion.

1984 • 17 citations

Dielectrophoretic behaviour ofSaccharomyces cerevisiae: effect of cations and detergents

1984 • 17 citations

Morphological and genetic effects of benomyl on polyploid brewing yeasts: isolation of auxotrophic mutants

1984 • 16 citations

Chromium resistant mutants of the yeast Saccharomyces cerevisiae

1982 • 16 citations

Valuable Techniques in the Genetic Manipulation of Industrial Yeast Strains

1985 • 16 citations

Fusion of yeast protoplasts.

1977 • 15 citations

Utilization of a Respiratory-Deficient Mutant for Alcohol Production

1982 • 15 citations

Use of Lipase and Strepzyme for the Isolation of ‘Protoplasts’ from Fungal Mycelium

1966 • 15 citations

Applied Aspects of Microbial Genetics

1970 • 14 citations

INTERGENERIC FUSION OF YEAST PROTOPLASTS

1981 • 14 citations

Efficiency of genetically engineered yeast in the production of ethanol from dextrinized cassava starch

1986 • 14 citations

Dielectrophoretic behaviour of Saccharomyces cerevisiae: effect of cations and detergents

1984 • 14 citations

Annual Review of Microbiology

1980 • 12 citations

Ethanol and biomass production of wild strains and respiratory deficient mutants of Saccharomyces cerevisiae under anaerobic and aerobic conditions

1982 • 11 citations

Control of Mitochondrial Inactivation of Temperature-sensitive Saccharomyces cerevisiae Nuclear Petite Mutants

1977 • 10 citations

INDUCTION OF RESPIRATION-DEFICIENT MUTANTS OF SACCHAROMYCES AND EVALUATION OF THEIR EFFICIENCY FOR ETHANOL PRODUCTION

1978 • 10 citations

ASPECTS OF MITOCHONDRIAL CONTROL OF CELL SURFACE CHARACTERISTICS IN SACCHAROMYCES CEREVISIAE

1981 • 9 citations

Interspecific protoplast Fusion of Schwanniomyces yeasts

1983 • 9 citations

Saccharomyces cerevisiae strains sensitive to inorganic mercury

1987 • 7 citations

Use of D‐glucosamine and 2‐deoxyglucose in the selective isolation of mutants of the yeast Lipomyces starkeyi derepressed for the production of extracellular endodextranase

1983 • 6 citations

Use of D-glucosamine and 2-deoxyglucose in the selective isolation of mutants of the yeastLipomyces starkeyi derepressed for the production of extracellular endodextranase

1983 • 6 citations

A study on the control of carbon catabolite-repressed proteins in Saccharomyces cerevisiae

1985 • 4 citations

Ethanol Production Characteristics for a Respiratory Deficient Mutant Yeast Strain

1982 • 3 citations

Saccharomyces cerevisiae strains sensitive to inorganic mercury

1985 • 3 citations

Effect of methanol on the induction of respiration-deficient mutants in yeast by acriflavine.

1984 • 2 citations