Cloning in Yeast

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

1984 • 2,401 citations

DNA cloning : a practical approach

1985 • 2,302 citations

Transformation of yeast.

1978 • 2,267 citations

High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.

1979 • 1,504 citations

Yeast transformation: a model system for the study of recombination.

1981 • 1,491 citations

Transformation of yeast by a replicating hybrid plasmid

1978 • 1,362 citations

Synthesis and assembly of hepatitis B virus surface antigen particles in yeast

1982 • 854 citations

Isolation and characterisation of a yeast chromosomal replicator

1979 • 795 citations

Isolation of a yeast centromere and construction of functional small circular chromosomes

1980 • 788 citations

Replacement of chromosome segments with altered DNA sequences constructed in vitro.

1979 • 779 citations

Methods in yeast genetics, laboratory manual

1987 • 733 citations

The Molecular biology of the yeast saccharomyces, life cycle and inheritance

1981 • 636 citations

Cloning yeast telomeres on linear plasmid vectors

1982 • 625 citations

[49] Rapid DNA isolations for enzymatic and hybridization analysis

1980 • 572 citations

The Saccharomyces cerevisiae Cell Cycle

1981 • 539 citations

[14] Genetic applications of yeast transformation with linear and gapped plasmids

1983 • 503 citations

Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.

1984 • 477 citations

Construction of artificial chromosomes in yeast

1983 • 448 citations

Yeast recombination: the association between double-strand gap repair and crossing-over.

1983 • 432 citations

Isolation of the β-tubulin gene from yeast and demonstration of its essential function in vivo

1983 • 409 citations

Genetic map of Saccharomyces cerevisiae

1980 • 394 citations

Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae

1983 • 337 citations

Expression of a human gene for interferon in yeast

1981 • 332 citations

[13] Eviction and transplacement of mutant genes in yeast

1983 • 305 citations

Functional expression of cloned yeast DNA in Escherichia coli.

1977 • 284 citations

Functional genetic expression of eukaryotic DNA in Escherichia coli.

1976 • 261 citations

The new yeast genetics

1983 • 200 citations

Yeast plasmid requires a cis-acting locus and two plasmid proteins for its stable maintenance

1983 • 167 citations

The yeast plasmid 2μ circle encodes components required for its high copy propagation

1983 • 163 citations

Regulated high efficiency expression of human interferon-alpha in Saccharomyces cerevisiae.

1982 • 139 citations

Secretion of foreign proteins from Saccharomyces cerevisiae directed by alpha-factor gene fusions.

1984 • 126 citations

The yeast ARS element, six years on: A progress report

1985 • 114 citations

Abnormal expression of chromosomal rabbit β-globin gene in Saccharomyces cerevisiae

1980 • 110 citations

The transformation of brewing yeasts with a plasmid containing the gene for copper resistance

1985 • 95 citations

Regulated expression of a human interferon gene in yeast: control by phosphate concentration or temperature.

1984 • 85 citations

Expression of the human interferon–γ cDNA in yeast

1983 • 65 citations