Mutants of the membrane-binding region of Semliki Forest virus E2 protein. I. Cell surface transport and fusogenic activity.

Molecular Cloning: A Laboratory Manual

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1982 • 22,471 citations

Studies on transformation of Escherichia coli with plasmids

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[2] New M13 vectors for cloning

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pEMBL: a new family of single stranded plasmids

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[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors

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Mechanisms for the incorporation of proteins in membranes and organelles.

1982 • 875 citations

Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

1977 • 711 citations

Membrane fusion proteins of enveloped animal viruses

1983 • 670 citations

Two mRNAs with different 3′ ends encode membrane-bound and secreted forms of immunoglobulin μ chain

1980 • 659 citations

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1984 • 563 citations

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1980 • 562 citations

The gapped duplex DNA approach to oligonucleotide-directed mutation construction

1984 • 511 citations

Mutation in LDL Receptor: Alu-Alu Recombination Deletes Exons Encoding Transmembrane and Cytoplasmic Domains

1985 • 453 citations

Nucleotide sequence of cDNA coding for Semliki Forest virus membrane glycoproteins

1980 • 428 citations

Membrane assembly in vitro : Synthesis, glycosylatio, and asymmetric insertion of a transmembrane protein

1977 • 366 citations

Influenza virus proteins

1970 • 341 citations

Domain structure of 3-hydroxy-3-methylglutaryl coenzyme A reductase, a glycoprotein of the endoplasmic reticulum.

1985 • 301 citations

Expression from cloned cDNA of cell-surface secreted forms of the glycoprotein of vesicular stomatitis virus in eucaryotic cells

1982 • 266 citations

A signal sequence for the insertion of a transmembrane glycoprotein. Similarities to the signals of secretory proteins in primary structure and function.

1978 • 266 citations

Altered cytoplasmic domains affect intracellular transport of the vesicular stomatitis virus glycoprotein

1983 • 264 citations

Sorting of an apical plasma membrane glycoprotein occurs before it reaches the cell surface in cultured epithelial cells.

1984 • 247 citations

Construction of influenza haemagglutinin genes that code for intracellular and secreted forms of the protein

1982 • 236 citations

Using Recombinant DNA Techniques to Study Protein Targeting in the Eucaryotic Cell

1985 • 158 citations

Fine structure of a membrane anchor domain

1985 • 151 citations

Secretion of a transplantation-related antigen

1983 • 150 citations

Mutations in the cytoplasmic domain of the influenza virus hemagglutinin affect different stages of intracellular transport.

1985 • 145 citations

A stop transfer sequence confers predictable transmembrane orientation to a previously secreted protein in cell-free systems

1983 • 142 citations

Evidence from cDNA clones that the rat leukocyte-common antigen (T200) spans the lipid bilayer and contains a cytoplasmic domain of 80,000 Mr

1985 • 132 citations

Subunit structure, cell surface orientation, and partial amino-acid sequences of murine histocompatibility antigens.

1976 • 128 citations

Herpes simplex virus type 1 glycoprotein C-negative mutants exhibit multiple phenotypes, including secretion of truncated glycoproteins

1984 • 122 citations

Conversion of a secretory protein into a transmembrane protein results in its transport to the golgi complex but not to the cell surface

1984 • 120 citations

Mutations of the Rous sarcoma virus env gene that affect the transport and subcellular location of the glycoprotein products.

1984 • 109 citations

Expression and function of transplantation antigens with altered or deleted cytoplasmic domains

1983 • 105 citations

A prokaryotic membrane anchor sequence: carboxyl terminus of bacteriophage f1 gene III protein retains it in the membrane.

1982 • 94 citations

Incorporation of a charged amino acid into the membrane-spanning domain blocks cell surface transport but not membrane anchoring of a viral glycoprotein.

1985 • 93 citations

Directed mutagenesis of DNA cloned in filamentous phage: influence of hemimethylated GATC sites on marker recovery from restriction fragments

1982 • 93 citations

Expression of Semliki Forest virus proteins from cloned complementary DNA. I. The fusion activity of the spike glycoprotein.

1983 • 91 citations

Isolation and characterization of the hydrophobic COOH-terminal domains of the Sindbis virion glycoproteins

1982 • 79 citations

Human and murine class I MHC antigens share conserved serine 335, the site of HLA phosphorylation in vivo.

1984 • 74 citations

The amphiphilic membrane glycoproteins of Semliki forest virus are attached to the lipid bilayer by their COOH-terminal ends

1978 • 74 citations

Semliki Forest Virus: A Probe for Membrane Traffic in the Animal Cell

1984 • 73 citations

Replication of a plasmid containing two origins of bacteriophage f1

1981 • 62 citations

Expression of Semliki Forest virus proteins from cloned complementary DNA. II. The membrane-spanning glycoprotein E2 is transported to the cell surface without its normal cytoplasmic domain.

1983 • 61 citations

Evidence for a separate signal sequence for the carboxy-terminal envelope glycoprotein E1 of Semliki forest virus

1981 • 57 citations

SOS mutator effect in E. coli mutants deficient in mismatch correction.

1984 • 52 citations

Incorporation of a Charged Amino Acid into the Membrane-Spanning Domain Blocks Cell Surface Transport But Not Membrane Anchoring of a Viral Glycoprotein

1985 • 41 citations

Pushing the signal hypothesis: what are the limits?

1985 • 37 citations

Interactions of Semliki Forest virus spike glycoprotein rosettes and vesicles with cultured cells.

1983 • 35 citations

Chemical synthesis and molecular cloning of a STOP oligonucleotide encoding an UGA translation terminator in all three reading frames

1983 • 34 citations

Chimeric influenza virus hemagglutinin containing either the NH2 terminus or the COOH terminus of G protein of vesicular stomatitis virus is defective in transport to the cell surface.

1984 • 32 citations

Sequence analysis of two mutants of sindbis virus defective in the intracellular transport of their glycoproteins

1983 • 30 citations

[39] Expression of viral membrane proteins from cloned cDNA by microinjection into eukaryotic cell nuclei

1983 • 28 citations

MECHANISMS FOR THE INTRACELLULAR COMPARTMENTATION OF NEWLY SYNTHESIZED PROTEINS

1978 • 10 citations

Different membrane anchors allow the Semliki Forest virus spike subunit E2 to reach the cell surface

1985 • 9 citations