Targeting of human catalase to peroxisomes is dependent upon a novel COOH-terminal peroxisomal targeting sequence.

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Methods in yeast genetics

1979 • 7,824 citations

Peroxisomes (microbodies and related particles).

1966 • 1,635 citations

The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.

1991 • 1,489 citations

A conserved tripeptide sorts proteins to peroxisomes.

1989 • 1,113 citations

Biogenesis of Peroxisomes

1985 • 1,108 citations

BIOCHEMISTRY OF PEROXISOMES

1992 • 843 citations

Construction of plasmids that expressE.coli β-galactosidase in mammalian cells

1989 • 458 citations

Mutations in the PTS1 receptor gene, PXR1, define complementation group 2 of the peroxisome biogenesis disorders

1995 • 430 citations

A Human Gene Responsible for Zellweger Syndrome That Affects Peroxisome Assembly

1992 • 357 citations

An oligomeric protein is imported into peroxisomes in vivo.

1994 • 340 citations

Identification of peroxisomal targeting signals located at the carboxy terminus of four peroxisomal proteins.

1988 • 338 citations

Peroxisomal Membrane Ghosts in Zellweger Syndrome—Aberrant Organelle Assembly

1988 • 327 citations

Isolation of peroxisome-deficient mutants of Saccharomyces cerevisiae.

1989 • 326 citations

Peroxisomal protein import is conserved between yeast, plants, insects and mammals.

1990 • 296 citations

Disorders of peroxisome biogenesis

1995 • 289 citations

Biogenesis of peroxisomes: intracellular site of synthesis of catalase and uricase.

1978 • 284 citations

Copper metallothionein of yeast, structure of the gene, and regulation of expression.

1984 • 283 citations

The pas8 mutant of Pichia pastoris exhibits the peroxisomal protein import deficiencies of Zellweger syndrome cells--the PAS8 protein binds to the COOH-terminal tripeptide peroxisomal targeting signal, and is a member of the TPR protein family [published erratum appears in J Cell Biol 1993 Sep;122(5):following 1143]

1993 • 246 citations

Phenotype of patients with peroxisomal disorders subdivided into sixteen complementation groups

1995 • 246 citations

PAS10 is a tetratricopeptide-repeat protein that is essential for the import of most matrix proteins into peroxisomes of Saccharomyces cerevisiae.

1993 • 225 citations

Activity of peroxisomal enzymes and intracellular distribution of catalase in Zellweger syndrome

1984 • 200 citations

THE SYNTHESIS AND TURNOVER OF RAT LIVER PEROXISOMES

1973 • 199 citations

Peroxisomal multifunctional beta-oxidation protein of Saccharomyces cerevisiae. Molecular analysis of the fox2 gene and gene product.

1992 • 195 citations

Human peroxisomal targeting signal-1 receptor restores peroxisomal protein import in cells from patients with fatal peroxisomal disorders.

1995 • 187 citations

The Pichia pastoris peroxisomal protein PAS8p is the receptor for the C-terminal tripeptide peroxisomal targeting signal.

1995 • 178 citations

Identification and Characterization of the Putative Human Peroxisomal C-terminal Targeting Signal Import Receptor

1995 • 176 citations

Genetic heterogeneity in the cerebrohepatorenal (Zellweger) syndrome and other inherited disorders with a generalized impairment of peroxisomal functions. A study using complementation analysis.

1988 • 164 citations

Two independent peroxisomal targeting signals in catalase A of Saccharomyces cerevisiae.

1993 • 158 citations

The targeting and assembly of peroxisomal proteins: some old rules do not apply

1996 • 157 citations

In vivo import of firefly luciferase into the glycosomes of Trypanosoma brucei and mutational analysis of the C-terminal targeting signal.

1992 • 148 citations

The Tetratricopeptide Repeat Domain of the PAS10 Protein of Saccharomyces cerevisiae Is Essential for Binding the Peroxisomal Targeting Signal -SKL

1994 • 147 citations

Association of glyoxylate and beta-oxidation enzymes with peroxisomes of Saccharomyces cerevisiae

1990 • 136 citations

The Hansenula polymorpha PER3 Gene Is Essential for the Import of PTS1 Proteins into the Peroxisomal Matrix

1995 • 130 citations

Isolation of peroxisome assembly mutants from Saccharomyces cerevisiae with different morphologies using a novel positive selection procedure.

1992 • 123 citations

Synthesis of catalase in two cell-free protein-synthesizing systems and in rat liver

1978 • 121 citations

Carboxyl-terminal consensus Ser-Lys-Leu-related tripeptide of peroxisomal proteins functions in vitro as a minimal peroxisome-targeting signal.

1992 • 119 citations

Glycosome assembly in trypanosomes: variations in the acceptable degeneracy of a COOH-terminal microbody targeting signal.

1992 • 116 citations

Biosynthesis and maturation of peroxisomal beta-oxidation enzymes in fibroblasts in relation to the Zellweger syndrome and infantile Refsum disease.

1986 • 106 citations

Sequence of the Saccharomyces cerevisiae CATI gene and amino acid sequence of catalase A derived from it

1988 • 104 citations

Isolation and characterization of the yeast gene encoding the MDH3 isozyme of malate dehydrogenase.

1992 • 101 citations

Peroxisomal biogenesis: multiple pathways of protein import.

1994 • 97 citations

The carboxyl-terminal tripeptide Ala-Lys-Ile is essential for targeting Candida tropicalis trifunctional enzyme to yeast peroxisomes.

1991 • 97 citations

Differentially regulated malate synthase genes participate in carbon and nitrogen metabolism ofS.cerevisiae

1992 • 94 citations

Mammalian alanine/glyoxylate aminotransferase 1 is imported into peroxisomes via the PTS1 translocation pathway. Increased degeneracy and context specificity of the mammalian PTS1 motif and implications for the peroxisome-to-mitochondrion mistargeting of AGT in primary hyperoxaluria type 1.

1995 • 93 citations

Complementation study of peroxisome-deficient disorders by immunofluorescence staining and characterization of fused cells

1992 • 92 citations

An efficient positive selection procedure for the isolation of peroxisomal import and peroxisome assembly mutants of Saccharomyces cerevisiae.

1993 • 92 citations

Isolation of human fibroblast catalase cDNA clones. Sequence of clones derived from spliced and unspliced mRNA.

1984 • 90 citations

An internal region of the peroxisomal membrane protein PMP47 is essential for sorting to peroxisomes

1994 • 84 citations

Zellwegar Syndrome: Biochemical and Morphological Studies on Two Patients Treated with Clofibrate

1985 • 84 citations

Efficient transformation of human fibroblasts by adenovirus-simian virus 40 recombinants.

1984 • 80 citations

cDNA sequence coding for human kidney catalase.

1986 • 73 citations

Peroxisomes in Saccharomyces cerevisiae: immunofluorescence analysis and import of catalase A into isolated peroxisomes.

1991 • 69 citations

Catalase in guinea pig hepatocytes is localized in cytoplasm, nuclear matrix and peroxisomes.

1988 • 66 citations

Chapter 23 Transmission Electron Microscopy and Immunocytochemical Studies of Yeast: Analysis of HMG—CoA Reductase Overproduction by Electron Microscopy

1989 • 65 citations

Localization of Catalase A in Vacuoles ofSaccharomyces cerevisiae:Evidence for the Vacuolar Nature of Isolated "Yeast Peroxisomes"

1976 • 63 citations

Targeting signal of the peroxisomal catalase in the methylotrophic yeastHansenula polymorpha

1992 • 62 citations

Novel peroxisome clustering mutants and peroxisome biogenesis mutants of Saccharomyces cerevisiae.

1993 • 58 citations

Targeting sequences of the two major peroxisomal proteins in the methylotrophic yeast Hansenula polymorpha

1992 • 56 citations

[26] Induced expression using yeast copper metallothionein promoter

1990 • 51 citations

Kinetics of the assembly of peroxisomes after fusion of complementary cell lines from patients with the cerebro-hepato-renal (Zellweger) syndrome and related disorders

1988 • 46 citations

Antibodies directed against a yeast carboxyl‐terminal peroxisomal targeting signal specifically recognize peroxisomal proteins from various yeasts

1992 • 40 citations

Topology of catalase assembly in human skin fibroblasts

1993 • 37 citations

The PAH2 gene is required for peroxisome assembly in the methylotrophic yeast Hansenula polymorpha and encodes a member of the tetratricopeptide repeat family of proteins

1995 • 36 citations

Expression and Function of a Mislocalized Form of Peroxisomal Malate Dehydrogenase (MDH3) in Yeast

1995 • 30 citations

Efficient Transformation of Human Fibroblasts by Adenovirus-Simian Virus 40 Recombinants

1984 • 26 citations

Alternative topogenic signals in peroxisomal citrate synthase of Saccharomyces cerevisiae.

1992 • 26 citations

Identification of peroxisomal membrane ghosts with an epitope‐tagged integral membrane protein in yeast mutants lacking peroxisomes

1995 • 24 citations

Peroxisomes in Saccharomyces cerevisiae: Immunofluorescence Analysis and Import of Catalase A into Isolated Peroxisomes

1991 • 20 citations

Catalase in cultured skin fibroblasts from patients with the cerebro-hepato-renal (Zellweger) syndrome: normal maturation in peroxisome-deficient cells

1987 • 17 citations

Alternative Topogenic Signals in Peroxisomal Citrate Synthase of Saccharomyces cerevisiae

1992 • 6 citations

Human catalase is imported and assembled in peroxisomes of Saccharomyces cerevisiae

1993 • 5 citations