Heterologous Expression of Genes in Bacterial, Fungal, Animal, and Plant Cells

Universal control mechanism regulating onset of M-phase

1990 • 2,882 citations

Direct expression cloning of vascular cell adhesion molecule 1, a cytokine-induced endothelial protein that binds to lymphocytes

1989 • 1,715 citations

Transformation of yeast by a replicating hybrid plasmid

1978 • 1,362 citations

Tobacco BY-2 Cell Line as the “HeLa” Cell in the Cell Biology of Higher Plants

1992 • 1,188 citations

Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2

1987 • 1,153 citations

Cloning by functional expression of a member of the glutamate receptor family

1989 • 1,009 citations

Expression cloning of a mammalian proton-coupled oligopeptide transporter

1994 • 880 citations

Expression cloning of an activin receptor, a predicted transmembrane serine kinase

1991 • 760 citations

The Use ofXenopusOocytes for the Study of Ion Channel

1987 • 649 citations

Transport of cationic amino acids by the mouse ecotropic retrovirus receptor

1991 • 552 citations

Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter

1986 • 480 citations

Isolation and characterization of a sucrose carrier cDNA from spinach by functional expression in yeast.

1992 • 479 citations

Evidence for an essential role of the sucrose transporter in phloem loading and assimilate partitioning.

1994 • 418 citations

Identification of a high affinity NH4+ transporter from plants.

1994 • 411 citations

The human oestrogen receptor functions in yeast

1988 • 402 citations

CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells.

1991 • 390 citations

Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system

1994 • 336 citations

The ABC-transporter Ste6 accumulates in the plasma membrane in a ubiquitinated form in endocytosis mutants.

1994 • 319 citations

[14] Cloning genes by complementation in yeast

1991 • 266 citations

Voltage-sensitive ion channels

1989 • 265 citations

Potato sucrose transporter expression in minor veins indicates a role in phloem loading.

1993 • 251 citations

Cloning and functional expression in yeast of two human isoforms of the outer mitochondrial membrane channel, the voltage-dependent anion channel.

1993 • 239 citations

Screening patients for heterozygous p53 mutations using a functional assay in yeast

1993 • 236 citations

Isolation of an Arabidopsis thaliana gene encoding cycloartenol synthase by functional expression in a yeast mutant lacking lanosterol synthase by the use of a chromatographic screen.

1993 • 226 citations

Cloning of a rat kidney cDNA that stimulates dibasic and neutral amino acid transport and has sequence similarity to glucosidases.

1992 • 222 citations

Complementation of snf1, a mutation affecting global regulation of carbon metabolism in yeast, by a plant protein kinase cDNA.

1991 • 211 citations

Cloning of a human cDNA encoding a CDC2-related kinase by complementation of a budding yeast cdc28 mutation.

1991 • 201 citations

Functional characterization of the Escherichia coli glycerol facilitator, GlpF, in Xenopus oocytes.

1994 • 185 citations

Novel voltage clamp to record small, fast currents from ion channels expressed in Xenopus oocytes

1992 • 180 citations

STRUCTURE AND FUNCTION OF HEXOSE TRANSPORTERS

1991 • 165 citations

Methylamine/ammonia uptake systems in Saccharomyces cerevisiae: multiplicity and regulation

1979 • 152 citations

Expression of a tuber-specific storage protein in transgenic tobacco plants: demonstration of an esterase activity

1987 • 152 citations

Expression of mRNA coding for kidney and red cell water channels in Xenopus oocytes.

1990 • 147 citations

NPK1, a tobacco gene that encodes a protein with a domain homologous to yeast BCK1, STE11, and Byr2 protein kinases.

1993 • 130 citations

Functional comparisons between plant plasma membrane H(+)-ATPase isoforms expressed in yeast.

1994 • 126 citations

Fission yeast and a plant have functional homologues of the Sar1 and Sec12 proteins involved in ER to Golgi traffic in budding yeast.

1992 • 124 citations

Abnormal expression of chromosomal rabbit β-globin gene in Saccharomyces cerevisiae

1980 • 110 citations

Cloning of an Arabidopsis histidine transporting protein related to nitrate and peptide transporters

1994 • 106 citations

Isolation of a gene from Drosophila by complementation in yeast

1981 • 95 citations

Inward rectifier potassium channels in plants differ from their animal counterparts in response to voltage and channel modulators

1995 • 93 citations

Phenylglucosides and the Na+/glucose cotransporter (SGLT1): Analysis of interactions

1994 • 93 citations

Biochemical, molecular, and functional characterization of porin isoforms from potato mitochondria

1994 • 92 citations

Evidence for a common structure for a class of membrane channels

1993 • 87 citations

cdc2MsB, a cognate cdc2 gene from alfalfa, complements the G1/S but not the G2/M transition of budding yeast cdc28 mutants

1993 • 67 citations

Saccharomyces cerevisiae cdc15 mutants arrested at a late stage in anaphase are rescued by Xenopus cDNAs encoding N-ras or a protein with beta-transducin repeats.

1993 • 66 citations

Baculovirus expression of the maize mitochondrial protein URF13 confers insecticidal activity in cell cultures and larvae.

1993 • 65 citations

Biosynthesis of the Torpedo californica Acetylcholine Receptor α Subunit in Yeast

1986 • 57 citations

Use of a yeast expression system for the isolation and analysis of drug-resistant mutants of a mammalian phosphodiesterase.

1993 • 56 citations

Complementation of the cs dis2-11 cell cycle mutant of Schizosaccharomyces pombe by a protein phosphatase from Arabidopsis thaliana.

1992 • 53 citations

Functional expression of a plant plasma membrane transporter in Xenopus oocytes

1992 • 50 citations

Molecular physiology of anion channels

1994 • 46 citations

Cloning and expression in yeast of a higher plant chorismate mutase Molecular cloning, sequencing of the cDNA and characterization of theArabidopsis thalianaenzyme expressed in yeast

1993 • 41 citations

Characterization of cDNA encoding the mouse DNA topoisomerase II that can complement the budding yeasttop2mutation

1992 • 36 citations

Expression of the two maize TATA binding protein genes and function of the encoded TBP proteins by complementation in yeast.

1993 • 27 citations

Cloning and sequencing of a human cDNA coding for dihydroorotate dehydrogenase by complementation of the corresponding yeast mutant

1992 • 26 citations

Isolation of a gene encoding a chaperonin-like protein by complementation of yeast amino acid transport mutants with human cDNA.

1992 • 22 citations

DNase I hypersensitive sites in the 5′-region of the maize Shrunken gene in nuclei from different organs

1988 • 14 citations

[27] Vaccinia virus as vector to express ion channel genes

1992 • 10 citations

Approaches to cloning genes encoding for nutrient transporters in plants

1993 • 7 citations

NIH 3T3 cells transfected with a yeast H⁺‐ATPase have altered sensitivity to insulin, insulin growth factor‐I, and platelet‐derived growth factor‐AA

1994 • 6 citations

A test for screening monoclonal antibodies to membrane proteins based on their ability to inhibit protein reconstitution into vesicles

1993 • 2 citations

Deleted Work

1955 • 0 citations