Production of infectious RNA transcripts from Sindbis virus cDNA clones: mapping of lethal mutations, rescue of a temperature-sensitive marker, and in vitro mutagenesis to generate defined mutants

Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues

2013 • 47,043 citations

[57] Sequencing end-labeled DNA with base-specific chemical cleavages

1980 • 15,302 citations

Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter

1984 • 6,459 citations

Quantitative Film Detection of 3H and 14C in Polyacrylamide Gels by Fluorography

1975 • 4,693 citations

Functional messenger RNAs are produced by SP6in vitrotranscription of cloned cDNAs

1984 • 1,533 citations

Cloned Poliovirus Complementary DNA Is Infectious in Mammalian Cells

1981 • 686 citations

Recognition of cap structure in splicing in vitro of mRNA precursors

1984 • 575 citations

3′-Terminal labelling of RNA with T4 RNA ligase

1978 • 556 citations

A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels

1979 • 460 citations

EK2 Derivatives of Bacteriophage Lambda Useful in the Cloning of DNA from Higher Organisms: The λgt WES System

1977 • 415 citations

Complete nucleotide sequence of the genomic RNA of Sindbis virus

1984 • 393 citations

The Togaviridae and Flaviviridae

1986 • 367 citations

Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase.

1986 • 344 citations

The T=4 envelope of sindbis virus is organized by interactions with a complementary T=3 capsid

1987 • 337 citations

Multicomponent RNA plant virus infection derived from cloned viral cDNA

1984 • 300 citations

Synthesis of brome mosaic virus subgenomic RNA in vitro by internal initiation on (−)-sense genomic RNA

1985 • 272 citations

cDNA cloning of the complete genome of tobacco mosaic virus and production of infectious transcripts

1986 • 263 citations

Sindbis Virus: A Newly Recognized Arthropod-Transmitted Virus 1

1955 • 259 citations

Isolation and characterization of conditional-lethal mutants of Sindbis virus

1966 • 254 citations

Expression of bacterial chloramphenicol acetyltransferase gene in tobacco plants mediated by TMV-RNA

1987 • 237 citations

Bacteriophage SP6-specific RNA polymerase. I. Isolation and characterization of the enzyme.

1982 • 233 citations

Structure and Replication of the Alphavirus Genome

1986 • 219 citations

Bacterial Gene Inserted in an Engineered RNA Virus: Efficient Expression in Monocotyledonous Plant Cells

1986 • 215 citations

Mapping of sequences required for mouse neurovirulence of poliovirus type 2 Lansing

1986 • 202 citations

In vitromutagenesis of the putative replicase genes of tobacco mosaic virus

1986 • 201 citations

Genetic analysis of the attenuation phenotype of poliovirus type 1

1986 • 191 citations

Qβ DNA-containing hybrid plasmids giving rise to Qβ phage formation in the bacterial host

1978 • 187 citations

Complementation between temperature-sensitive mutants of Sindbis virus

1966 • 164 citations

Deletion mapping of sindbis virus DI RNAs derived from cDNAs defines the sequences essential for replication and packaging

1986 • 160 citations

Partial nucleotide sequence of the Murray Valley encephalitis virus genome

1986 • 155 citations

In vitro transcription of infectious RNAs from full-length cDNAs of tobacco mosaic virus

1986 • 147 citations

Association of Sindbis virion glycoproteins and their precursors

1982 • 133 citations

Genetic complementation among poliovirus mutants derived from an infectious cDNA clone

1986 • 132 citations

Construction of infectious potato spindle tuber viroid cDNA clones

1983 • 123 citations

Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals

1984 • 121 citations

Role of the Immune Response in Recovery from Sindbis Virus Encephalitis in Mice

1977 • 116 citations

Mutant viral RNAs synthesized in vitro show altered aminoacylation and replicase template activities

1984 • 115 citations

Sequence studies of several alphavirus genomic RNAs in the region containing the start of the subgenomic RNA.

1982 • 109 citations

Production of guanidine-resistant and -dependent poliovirus mutants from cloned cDNA: mutations in polypeptide 2C are directly responsible for altered guanidine sensitivity

1986 • 108 citations

A poliovirus temperature-sensitive RNA synthesis mutant located in a noncoding region of the genome.

1986 • 101 citations

Comparative studies of the 3′-terminal sequences of several alphavirus RNAs

1981 • 98 citations

A single nucleotide change in the E2 glycoprotein gene of Sindbis virus affects penetration rate in cell culture and virulence in neonatal mice.

1986 • 98 citations

Mutants of Sindbis virus

1976 • 93 citations

Expression of Sindbis virus structural proteins via recombinant vaccinia virus: synthesis, processing, and incorporation into mature Sindbis virions

1985 • 89 citations

In vitro synthesis of an infectious RNA from cDNA clones of human rhinovirus type 14

1985 • 88 citations

Infectivity andin vitromutagenesis of monomeric cDNA clones of citrus exocortis viroid indicates the site of processing of viroid precursors

1985 • 85 citations

Infectivity studies on different potato spindle tuber viroid (PSTV) RNAs synthesized in vitro with the SP6 transcription system

1985 • 83 citations

Sequence analysis of three Sindbis virus mutants temperature-sensitive in the capsid protein autoprotease.

1985 • 81 citations

Modulation of replication, aminoacylation and adenylation in vitro and infectivity in vivo of BMV RNAs containing deletions within the multifunctional 3′ end.

1986 • 80 citations

Infectious RNA derived by transcription from cloned cDNA copies of the genomic RNA of an insect virus.

1986 • 77 citations

A sindbis virus mutant temperature-sensitive in the regulation of minus-strand RNA synthesis

1981 • 76 citations

Functional defects of temperature-sensitive mutants of Sindbis virus

1968 • 75 citations

Alphavirus Pathogenesis and Immunity

1986 • 73 citations

Effect of Ionic Strength on the Binding of Sindbis Virus to Chick Cells

1974 • 72 citations

Sindbis Virus Mutants Selected for Rapid Growth in Cell Culture Display Attenuated Virulence in Animals

1984 • 67 citations

Formation and Assembly of Alphavirus Glycoproteins

1986 • 67 citations

In vitro phenotypic markers of a poliovirus recombinant constructed from infectious cDNA clones of the neurovirulent Mahoney strain and the attenuated Sabin 1 strain

1985 • 63 citations

Establishment and maintenance of persistent infection by Sindbis virus in BHK cells

1980 • 62 citations

Cloned infectious complementary DNA of the poliovirus Sabin 1 genome: biochemical and biological properties of the recovered virus

1984 • 62 citations

Entry of Alphaviruses

1986 • 62 citations

Functional analysis of the a complementation group mutants of sindbis HR virus

1985 • 61 citations

Synthesis, cleavage and sequence analysis of DNA complementary to the 26 S messenger RNA of Sindbis virus

1981 • 61 citations

Syrinx 2A: an improved lambda phage vector designed for screening DNA libraries by recombination in vivo.

1987 • 57 citations

Satellite tobacco ringspot virus RNA: Biological activity of DNA clones and their in vitro transcripts

1986 • 47 citations

Mutants of Alphaviruses: Genetics and Physiology

1980 • 45 citations

Expression of Alfalfa Mosaic virus RNA 4 cDNA transcripts in Vitro and in Vivo

1985 • 43 citations

In vitro synthesis of infectious poliovirus RNA.

1985 • 43 citations

Purification and amino acid compositions of the structural proteins of sindbis virus

1979 • 42 citations

Engineered defective interfering RNAs of Sindbis virus express bacterial chloramphenicol acetyltransferase in avian cells.

1987 • 40 citations

An Infectious cDNA clone of the poliovirus sabin strain could be used as a stable repository and inoculum for the oral polio live vaccine

1986 • 40 citations

Temperature-sensitive host-dependent mutants of Sindbis virus

1981 • 37 citations

Infectious RNA transcripts from cloned cDNAS of cucumber mosaic viral satellites

1986 • 36 citations

Requirement for host transcription in the replication of Sindbis virus

1983 • 34 citations

Mutants of sindbis virus II. Characterization of a maturation-defective mutant, ts103

1977 • 33 citations

Synthesis in vitro of infectious RNA copies of the virulent satellite of turnip crinkle virus

1987 • 31 citations

Cytopathic Effect and Plaque Formation by Arboviruses in a Continuous Cell Line (XTC-2) from the Toad Xenopus laevis

1977 • 31 citations

The effect of loss of regulation of minus-strand RNA synthesis on sindbis virus replication

1986 • 30 citations

Sequence analysis of two mutants of sindbis virus defective in the intracellular transport of their glycoproteins

1983 • 30 citations

Studies on SP6 promoter using a new plasmid vector that allow gene insertion at the transcription initiation site

1987 • 26 citations

Sindbis virus mutant ts20 of complementation group E contains a lesion in glycoprotein E2

1986 • 25 citations

Sindbis virus mutants able to replicate in methionine-deprived Aedes albopictus cells

1985 • 24 citations

Comparative characterization of a C-type virus-producing cell line (VSW) and a virus-free cell line (VH2) from Vipera russelli.

1973 • 21 citations

Regulated expression of Sindbis and vesicular stomatitis virus glycoproteins in Saccharomyces cerevisiae.

1986 • 19 citations

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