Secondary structure prediction and in vitro accessibility of mRNA as tools in the selection of target sites for ribozymes

Practical Statistics for Medical Research.

1992 • 12,008 citations

Practical Statistics for Medical Research

1990 • 9,667 citations

Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure

1999 • 3,775 citations

Algorithms and Thermodynamics for RNA Secondary Structure Prediction: A Practical Guide

1999 • 1,146 citations

Polymer support oligonucleotide synthesis XVIII^1.2): use ofβ-cyanoethyi-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product

1984 • 840 citations

The automated chemical synthesis of long oligoribuncleotides using 2'-O-silylated ribonucleoside 3'-O-phosphoramidites on a controlled-pore glass support: synthesis of a 43-nucleotide sequence similar to the 3'-half molecule of an Escherichia coli formylmethionine tRNA

1987 • 367 citations

Numbering system for the hammerhead

1992 • 291 citations

Selecting effective antisense reagents on combinatorial oligonucleotide arrays

1997 • 267 citations

Protein Enhancement of Hammerhead Ribozyme Catalysis

1993 • 199 citations

Mapping of RNA accessible sites for antisense experiments with oligonucleotide libraries

1998 • 188 citations

Determining the influence of structure on hybridization using oligonucleotide arrays

1999 • 178 citations

Potent antisense oligonucleotides to the human multidrug resistance-1 mRNA are rationally selected by mapping RNA-accessible sites with oligonucleotide libraries

1996 • 168 citations

Effects of RNA secondary structure on cellular antisense activity

2000 • 162 citations

Selection of Efficient Cleavage Sites in Target RNAs by Using a Ribozyme Expression Library

1995 • 151 citations

Hammerhead ribozymes: importance of stem-loop II for activity.

1993 • 138 citations

A synthetic, chemically modified ribozyme eliminates amelogenin, the major translation product in developing mouse enamel in vivo.

1995 • 137 citations

Nuclease-resistant chimeric ribozymes containing deoxyribonucleotides and phosphorothioate linkages

1993 • 124 citations

Inhibition of hepatitis C virus (HCV)-RNA-dependent translation and replication of a chimeric HCV poliovirus using synthetic stabilized ribozymes

2000 • 116 citations

A theoretical approach to select effective antisense oligodeoxyribonucleotides at high statistical probability

1999 • 103 citations

Probing accessible sites for ribozymes on human acetylcholinesterase RNA.

1997 • 96 citations

Comparative analysis of cleavage rates after systematic permutation of the NUX↓ consensus target motif for hammerhead ribozymes

1995 • 95 citations

RNA accessibility prediction: a theoretical approach is consistent with experimental studies in cell extracts

2000 • 90 citations

Intracellular cleavage of hepatitis C virus RNA and inhibition of viral protein translation by hammerhead ribozymes.

1996 • 89 citations

Cytoplasmic delivery of ribozymes leads to efficient reduction in alpha-lactalbumin mRNA levels in C127I mouse cells.

1992 • 88 citations

Efficient hammerhead ribozyme-mediated cleavage of the structured heapatitis B virus encapsidation signalin vitroand in cell extracts, but not in intact cells

1995 • 85 citations

A rapid in vitro method for obtaining RNA accessibility patterns for complementary DNA probes: correlation with an intracellular pattern and known RNA structures

1997 • 84 citations

Rapid determination and quantitation of the accessibility to native RNAs by antisense oligodeoxynucleotides in murine cell extracts

1998 • 83 citations

Bioactivity of anti-angiogenic ribozymes targeting Flt-1 and KDR mRNA

1999 • 78 citations

Nuclease-resistant ribozymes decrease stromelysin mRNA levels in rabbit synovium following exogenous delivery to the knee joint.

1996 • 74 citations

Optimizing the Cell Efficacy of Synthetic Ribozymes

1996 • 73 citations

Computer-Aided Search for Effective Antisense RNA Target Sequences of the Human Immunodeficiency Virus Type 1

1993 • 68 citations

Ribozyme-mediated Suppression of the G Protein γ7Subunit Suggests a Role in Hormone Regulation of Adenylylcyclase Activity

1997 • 66 citations

Hammerhead ribozyme design and application

1998 • 59 citations

A ribozyme with DNA in the hybridising arms displays enhanced cleavage ability

1992 • 58 citations

Synthesis of 2′–modified nucleotides and their incorporation into hammerhead ribozymes

1995 • 56 citations

Specific Hammerhead Ribozyme-mediated Cleavage of Mutant N-ras mRNA in Vitro and ex Vivo

1997 • 55 citations

The effect of structure in a long target RNA on ribozyme cleavage efficiency

1997 • 52 citations

Complementary Large Loops Determine the Rate of RNA Duplex Formation in Vitro in the Case of an Effective Antisense RNA Directed Against the Human Immunodeficiency Virus Type 1

1993 • 44 citations

Ribozyme-Mediated Inhibition of Expression of Leukocyte-type 12-Lipoxygenase in Porcine Aortic Vascular Smooth Muscle Cells

1995 • 42 citations

In vitro selection of a purine nucleotide-specific hammerheadlike ribozyme

1998 • 41 citations

Mechanisms of Inhibition of in Vitro Dimerization of HIV Type I RNA by Sense and Antisense Oligonucleotides

1996 • 38 citations

Interaction Between Tumour Necrosis Factor α Ribozyme and Cellular Proteins

1994 • 37 citations

Extending the cleavage rules for the hammerhead ribozyme: Mutating adenosine15.1 to inosine15.1 changes the cleavage site specificity from N16.2U16.1H17 to N16.2C16.1H17

1998 • 37 citations

On the rapid deprotection of synthetic oligonucleotides and analogs

1994 • 37 citations

Synthesis of RNA containing inosine: analysis of the sequence requirements for the 5′ splice site of theTetrahymenagroup I intron

1991 • 35 citations

Efficient hammerhead ribozyme and antisense RNA targeting in a slow ribosome Escherichia colimutant

1997 • 35 citations

Fast and simple purification of chemically modified hammerhead ribozymes using a lipophilic capture tag

1999 • 31 citations

Inhibition of luciferase expression by synthetic hammerhead ribozymes and their cellular uptake

1999 • 30 citations

Comparative Analysis of Five Highly Conserved Target Sites Within the HIV-1 RNA for Their Susceptibility to Hammerhead Ribozyme-Mediated CleavageIn VitroandIn Vivo

1996 • 27 citations

Efficient Improvement of Hammerhead Ribozyme Mediated Cleavage of Long Substrates by Oligonucleotide Facilitators

1996 • 27 citations

Retrovirus-Mediated Transfer of Anti-MDR1 Ribozymes Fully Restores Chemosensitivity of P-Glycoprotein-Expressing Human Lymphoma Cells

1999 • 27 citations

Correlation of Activity with Stability of Chemically Modified Ribozymes in Nuclei Suspension

1996 • 22 citations

Retrovirus-mediated transfer of anti-MRD1 hammerhead ribozymes into multidrug-resistant human leukemia cells: Screening for effective target sites

1999 • 21 citations

Effects of deoxyribonucleotide substitutions in the substrate strand on hammerhead ribozyme-catalyzed reactions

1994 • 9 citations

Transcripts containing a small anti-HIV hammerhead ribozyme that are active in the cell cytoplasm but inactive in vitro as free RNAs

1996 • 8 citations

Rapid desilylation of oligoribonucleotides at elevated temperatures: cleavage activity in ribozyme-substrate assays.

1995 • 8 citations

Rapid deprotection procedures for synthetic oligonucleotides.

1991 • 7 citations

Application of hammerhead ribozymes for structural studies of ribosomal 5S RNAs

1997 • 3 citations

Cleavage of the highly conserved hairpin-loop region of HIV-1 by synthetic ribozymes.

1993 • 3 citations