Assays for monitoring cellular immune responses to active immunotherapy of cancer.

Real time quantitative PCR.

1996 • 6,155 citations

Immunobiology: the immune system in health and disease

1995 • 3,893 citations

Phenotypic Analysis of Antigen-Specific T Lymphocytes

1996 • 3,611 citations

Vaccination of melanoma patients with peptide- or tumorlysate-pulsed dendritic cells

1998 • 2,916 citations

Lymphocyte responses and cytokines

1994 • 1,860 citations

Vaccination with Mage-3a1 Peptide–Pulsed Mature, Monocyte-Derived Dendritic Cells Expands Specific Cytotoxic T Cells and Induces Regression of Some Metastases in Advanced Stage IV Melanoma

1999 • 1,260 citations

Infusion of Cytotoxic T Cells for the Prevention and Treatment of Epstein-Barr Virus–Induced Lymphoma in Allogeneic Transplant Recipients

1998 • 1,103 citations

Tumor regressions observed in patients with metastatic melanoma treated with an antigenic peptide encoded by geneMAGE-3 and presented by HLA-A1

1999 • 803 citations

Determination of antigen-specific memory/effector CD4+ T cell frequencies by flow cytometry: evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency.

1997 • 560 citations

Ex Vivo Staining of Metastatic Lymph Nodes by Class I Major Histocompatibility Complex Tetramers Reveals High Numbers of Antigen-experienced Tumor-specific Cytolytic T Lymphocytes

1998 • 534 citations

A Re-Evaluation of the Frequency of CD8+ T Cells Specific for EBV in Healthy Virus Carriers

1999 • 520 citations

Generation of Human Cytotoxic T Cells Specific for Human Carcinoembryonic Antigen Epitopes From Patients Immunized With Recombinant Vaccinia-CEA Vaccine

1995 • 506 citations

High Frequencies of Naive Melan-a/Mart-1–Specific Cd8+ T Cells in a Large Proportion of Human Histocompatibility Leukocyte Antigen (Hla)-A2 Individuals

1999 • 497 citations

Reverse ELISPOT assay for clonal analysis of cytokine production I. Enumeration of gamma-interferon-secreting cells

1988 • 496 citations

Induction of immunity to prostate cancer antigens: results of a clinical trial of vaccination with irradiated autologous prostate tumor cells engineered to secrete granulocyte-macrophage colony-stimulating factor using ex vivo gene transfer.

1999 • 468 citations

Rapid generation of broad T-cell immunity in humans after a single injection of mature dendritic cells

1999 • 466 citations

Isolation of High Avidity Melanoma-Reactive CTL from Heterogeneous Populations Using Peptide-MHC Tetramers

1999 • 423 citations

Monitoring CD8 T cell responses to NY-ESO-1: Correlation of humoral and cellular immune responses

2000 • 407 citations

Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay

1999 • 404 citations

Quantification of antigen specific CD8+ T cells using an ELISPOT assay

1995 • 367 citations

Eradication of Established Tumors by CD8+ T Cell Adoptive Immunotherapy

2000 • 349 citations

Generation of immunity to the HER-2/neu oncogenic protein in patients with breast and ovarian cancer using a peptide-based vaccine.

1999 • 339 citations

Epstein-Barr virus (EBV) load in bone marrow transplant recipients at risk to develop posttransplant lymphoproliferative disease: prophylactic infusion of EBV-specific cytotoxic T cells

2000 • 335 citations

A Phase I study of active immunotherapy with carcinoembryonic antigen peptide (CAP-1)-pulsed, autologous human cultured dendritic cells in patients with metastatic malignancies expressing carcinoembryonic antigen.

1999 • 299 citations

Increased Vaccine-Specific T Cell Frequency After Peptide-Based Vaccination Correlates with Increased Susceptibility to In Vitro Stimulation But Does Not Lead to Tumor Regression

1999 • 290 citations

Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry

1998 • 285 citations

Direct isolation, phenotyping and cloning of low-frequency antigen-specific cytotoxic T lymphocytes from peripheral blood

1998 • 234 citations

Longitudinal analysis of T cell receptor (TCR) gene usage by human immunodeficiency virus 1 envelope-specific cytotoxic T lymphocyte clones reveals a limited TCR repertoire.

1994 • 232 citations

Identification of functional subsets by flow cytometry: Intracellular detection of cytokine expression

1998 • 219 citations

A recombinant vaccinia virus based ELISPOT assay detects high frequencies of Pol-specific CD8 T cells in HIV-1-positive individuals

1999 • 217 citations

Functional Analysis of Antigen-Specific T Lymphocytes by Serial Measurement of Gene Expression in Peripheral Blood Mononuclear Cells and Tumor Specimens

1999 • 206 citations

Cytolytic T lymphocyte recognition of the immunodominant HLA-A*0201-restricted Melan-A/MART-1 antigenic peptide in melanoma.

1997 • 176 citations

Quantification of cytokine mRNA expression by RT PCR in samples of previously frozen blood

1997 • 169 citations

Vaccination with IL-12 gene-modified autologous melanoma cells: preclinical results and a first clinical phase I study

1998 • 167 citations

Signaling defects in T lymphocytes of patients with malignancy

1999 • 166 citations

In vitro predictors of therapeutic response in melanoma patients receiving tumor-infiltrating lymphocytes and interleukin-2.

1994 • 158 citations

Reverse ELISPOT assay for clonal analysis of cytokine production

1992 • 135 citations

A flow cytometric method to estimate the precursor frequencies of cells proliferating in response to specific antigens

1999 • 133 citations

Evaluation of the interferon-γ ELISPOT-assay for quantification of peptide specific T lymphocytes from peripheral blood

1997 • 133 citations

Immunotherapy of Metastatic Malignant Melanoma by a Vaccine Consisting of Autologous Interleukin 2-Transfected Cancer Cells: Outcome of a Phase I Study

1999 • 125 citations

Evidence of a Cellular Immune Response Against Sialyl-Tn in Breast and Ovarian Cancer Patients After High-Dose Chemotherapy, Stem Cell Rescue, and Immunization with Theratope STn-KLH Cancer Vaccine

1999 • 120 citations

A Phase I Vaccine Trial with Peptides Reflecting ras Oncogene Mutations of Solid Tumors

1999 • 117 citations

Delayed-type hypersensitivity response is a predictor of peripheral blood T-cell immunity after HER-2/neu peptide immunization.

2000 • 113 citations

Report of immune monitoring of prostate cancer patients undergoing T-cell therapy using dendritic cells pulsed with HLA-A2-specific peptides from prostate-specific membrane antigen (PSMA)

1998 • 113 citations

Rapid Determination of Epstein-Barr Virus–Specific CD8+T-Cell Frequencies by Flow Cytometry

1999 • 100 citations

T cell receptor (TCR) structure of autologous melanoma-reactive cytotoxic T lymphocyte (CTL) clones: tumor-infiltrating lymphocytes overexpress in vivo the TCR beta chain sequence used by an HLA-A2-restricted and melanocyte-lineage-specific CTL clone.

1993 • 98 citations

Vaccination with IL-7 gene-modified autologous melanoma cells can enhance the anti-melanoma lytic activity in peripheral blood of patients with a good clinical performance status: a clinical phase I study

1998 • 98 citations

Delayed cutaneous hypersensitivity to autologous tumor cells in colorectal cancer patients immunized with an autologous tumor cell: Bacillus Calmette-Guérin vaccine.

1984 • 96 citations

Novel methods to monitor antigen-specific cytotoxic T-cell responses in cancer immunotherapy

1998 • 86 citations

Expansion of Tumor-T Cell Pairs from Fine Needle Aspirates of Melanoma Metastases

2000 • 76 citations

High-sensitivity immunofluorescence for detection of the pro- and anti-inflammatory cytokines gamma interferon and interleukin-10 on the surface of cytokine-secreting cells

2000 • 69 citations

Multiple specificities in the repertoire of a melanoma patient's cytolytic T lymphocytes directed against tumor antigen MAGE-1.A1.

1995 • 68 citations

Possible improved survival of patients with stage IV AJCC melanoma receiving SRL 172 immunotherapy: Correlation with induction of increased levels of intracellular interkukin-2 in peripheral blood lymphocytes

1999 • 68 citations

Circulating MELAN-A/MART-1 specific cytolytic T lymphocyte precursors in HLA-A2+ melanoma patients have a memory phenotype

1998 • 67 citations

Antitumor Immunization with a Minimal Peptide Epitope (G9–209–2M) Leads to a Functionally Heterogeneous CTL Response

1999 • 67 citations

Stimulation of CD8+ T cell responses to MAGE-3 and Melan A/MART-1 by immunization to a polyvalent melanoma vaccine

1997 • 65 citations

Overexpression of the T-cell receptor beta-chain variable region TCRBV14 in HLA-A2-matched primary human melanomas.

1995 • 43 citations

Immunization With Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor as a Vaccine Adjuvant Elicits Both a Cellular and Humoral Response to Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor

1999 • 40 citations

Evidence for oligoclonal T-cell response in a metastasis of renal cell carcinoma responding to vaccination with autologous tumor cells and transfer of in vitro-sensitized vaccine-draining lymph node lymphocytes.

1993 • 39 citations

Induction of tumor-specific cytotoxic T lymphocytes by immunization with autologous tumor cells and interleukin-2 gene transfected fibroblasts

1997 • 39 citations

Development of a dual color enzyme-linked immunospot assay for simultaneous detection of murine T helper type 1- and T helper type 2-cells

1998 • 38 citations

Use of a skin test assay to determine tumor‐specific CD8⁺ T cell reactivity

1994 • 38 citations

Evaluation of Immunological Responses in Patients with Ovarian Cancer Treated with the Anti-idiotype Vaccine ACA125 by Determination of Intracellular Cytokines—A Preliminary Report

1999 • 33 citations

Detection of intracellular cytokines in citrated whole blood or marrow samples by flow cytometry

1999 • 29 citations

Digital Quantification of the Enzyme-Linked Immunospot (ELISPOT)

1998 • 26 citations

Melanoma-reactive human cytotoxic T lymphocytes derived from skin biopsies of delayed-type hypersensitivity reactions induced by injection of an autologous melanoma cell line.

1997 • 22 citations

Quantitation of T-Cell Receptor Frequencies by Competitive PCR: Generation and Evaluation of Novel TCR Subfamily and Clone Specific Competitors

1999 • 16 citations

Verminderte Progressionsrate und Induktion tumorspezifischer Immunantworten durch adjuvante Immuntherapie bei Stadium-IV-Melanomen

1999 • 15 citations

An Automated Method for the Analysis of T-Cell Receptor Repertoires: Rapid RT-PCR Fragment Length Analysis of the T-Cell Receptor β Chain Complementarity-Determining Region 3

1999 • 13 citations

Low-Dose Monoclonal Antibody CC49 Administered Sequentially with Granulocyte-Macrophage Colony-Stimulating Factor in Patients with Metastatic Colorectal Cancer

1999 • 7 citations

Immunological issues in vaccine trials: T-cell responses.

1998 • 4 citations