Structure-based Mutagenesis Reveals Distinct Functions for Ras Switch 1 and Switch 2 in Sos-catalyzed Guanine Nucleotide Exchange

Proteins regulating Ras and its relatives

1993 • 1,930 citations

Association of Sos Ras exchange protein with Grb2 is implicated in tyrosine kinase signal transduction and transformation

1993 • 1,236 citations

Epidermal growth factor regulates p21ras through the formation of a complex of receptor, Grb2 adapter protein, and Sos nucleotide exchange factor

1993 • 1,077 citations

The SH2 and SH3 domains of mammalian Grb2 couple the EGF receptor to the Ras activator mSos1

1993 • 1,026 citations

Guanine-nucleotide-releasing factor hSos1 binds to Grb2 and links receptor tyrosine kinases to Ras signalling

1993 • 972 citations

The structural basis of the activation of Ras by Sos

1998 • 765 citations

Multiple ras functions can contribute to mammalian cell transformation

1995 • 658 citations

Grb2 mediates the EGF-dependent activation of guanine nucleotide exchange on Ras

1993 • 587 citations

Structural Basis for Activation of ARF GTPase

1998 • 534 citations

Membrane targeting of the nucleotide exchange factor Sos is sufficient for activating the Ras signaling pathway

1994 • 458 citations

Crystal structure of Rac1 in complex with the guanine nucleotide exchange region of Tiam1

2000 • 356 citations

Kinetic Analysis by Fluorescence of the Interaction between Ras and the Catalytic Domain of the Guanine Nucleotide Exchange Factor Cdc25^Mm

1998 • 221 citations

Crystal Structure of Human RhoA in a Dominantly Active Form Complexed with a GTP Analogue

1998 • 214 citations

Mutational analysis of a ras catalytic domain.

1986 • 205 citations

The crystal structure of human rac1, a member of the rho-family complexed with a GTP analogue

1997 • 192 citations

Structural requirements for the interaction of p21ras with GAP, exchange factors, and its biological effector target

1993 • 189 citations

Structure of the Guanine Nucleotide Exchange Factor Sec7 Domain of Human Arno and Analysis of the Interaction with ARF GTPase

1998 • 184 citations

A glutamic finger in the guanine nucleotide exchange factor ARNO displaces Mg2+ and the beta -phosphate to destabilize GDP on ARF1

1998 • 180 citations

The Role of Mg2+ Cofactor in the Guanine Nucleotide Exchange and GTP Hydrolysis Reactions of Rho Family GTP-binding Proteins

2000 • 172 citations

Influence of guanine nucleotides on complex formation between Ras and CDC25 proteins.

1993 • 147 citations

The role of the PH domain in the signal-dependent membrane targeting of Sos

1997 • 131 citations

Mutations of Ha-ras p21 that define important regions for the molecular mechanism of the SDC25 C-domain, a guanine nucleotide dissociation stimulator.

1992 • 102 citations

Regulation of Sos Activity by Intramolecular Interactions

1998 • 98 citations

Identification of residues critical for Ras(17N) growth-inhibitory phenotype and for Ras interaction with guanine nucleotide exchange factors.

1994 • 90 citations

Definition of the Switch Surface in the Solution Structure of Cdc42Hs^,

1997 • 82 citations

Homeotic control in Drosophila; the scabrous gene is an in vivo target of Ultrabithorax proteins.

1992 • 81 citations

In Vivo Functional Analysis of the Ras Exchange Factor Son of Sevenless

1995 • 76 citations

Identification of Residues of the H-Ras Protein Critical for Functional Interaction with Guanine Nucleotide Exchange Factors

1994 • 75 citations

Functional Roles for the Pleckstrin and Dbl Homology Regions in the Ras Exchange Factor Son-of-sevenless

1995 • 65 citations

An Open Conformation of Switch I Revealed by the Crystal Structure of a Mg2+-free Form of RHOA Complexed with GDP

2000 • 61 citations

N Terminus of Sos1 Ras Exchange Factor: Critical Roles for the Dbl and Pleckstrin Homology Domains

1998 • 60 citations

RAS residues that are distant from the GDP binding site play a critical role in dissociation factor-stimulated release of GDP.

1992 • 55 citations

Involvement of the Switch 2 Domain of Ras in Its Interaction with Guanine Nucleotide Exchange Factors

1996 • 53 citations

The Sos (Son of sevenless) protein

1994 • 47 citations

Residues crucial for Ras interaction with GDP-GTP exchangers.

1993 • 45 citations

Identification of residues of the H-ras protein critical for functional interaction with guanine nucleotide exchange factors.

1994 • 45 citations

p21Ras activation by the guanine nucleotide exchange factor Sos, requires the Sos/Grb2 interaction and a second ligand-dependent signal involving the Sos N-terminus.

1996 • 41 citations

Yeast mutants temperature-sensitive for growth after random mutagenesis of the chromosomal RAS2 gene and deletion of the RAS1 gene.

1988 • 39 citations

Endocytosis and recycling of subunit H1 of the asialoglycoprotein receptor is independent of oligomerization with H2.

1989 • 36 citations

Identification of amino acids in p21ras involved in exchange factor interaction.

1993 • 34 citations

Discriminatory Residues in Ras and Rap for Guanine Nucleotide Exchange Factor Recognition

1999 • 32 citations

Distinct Subclasses of Small GTPases Interact with Guanine Nucleotide Exchange Factors in a Similar Manner

1998 • 27 citations

Characterization and Properties of Dominant-negative Mutants of the Ras-specific Guanine Nucleotide Exchange Factor CDC25Mm

1999 • 26 citations

Amino Acid Residues in the CDC25 Guanine Nucleotide Exchange Factor Critical for Interaction with Ras

1994 • 25 citations

The catalytic domain of the mouse sos1 gene product activates Ras proteins in vivo and in vitro.

1993 • 21 citations

Inhibition of mSOS-activity by binding of phosphatidylinositol 4,5-P2 to the mSOS pleckstrin homology domain

1998 • 16 citations

Activation of Ras and its downstream extracellular signal-regulated protein kinases by the CDC25 homology domain of mouse Son-of-sevenless 1 (mSos1)

1998 • 15 citations

Amino acid residues in the CDC25 guanine nucleotide exchange factor critical for interaction with Ras.

1994 • 14 citations

Rasp21 sequences opposite the nucleotide binding pocket are required for GRF-mediated nucleotide release.

1996 • 13 citations

Chromosomal localization of two genes encoding human ras exchange factors: SOS1 maps to the 2p22→p16 region and SOS2 to the 14q21→q22 region of the human genome

1994 • 11 citations