A novel essential fission yeast gene Pad1+ positively regulates Pap1+-dependent transcription and is implicated in the maintenance of chromosome structure

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

1979 • 54,907 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing

1984 • 4,231 citations

[56] Molecular genetic analysis of fission yeast Schizosaccharomyces pombe

1991 • 3,691 citations

[12] One-step gene disruption in yeast

1983 • 3,302 citations

Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis

1981 • 2,828 citations

Staurosporine, a potent inhibitor of phospholipidCa++dependent protein kinase

1986 • 2,445 citations

Methods in cell physiology

1964 • 1,816 citations

Dideoxy sequencing method using denatured plasmid templates

1986 • 1,537 citations

2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans

1994 • 1,472 citations

Vectors for selective expression of cloned DNAs by T7 RNA polymerase

1987 • 1,449 citations

The complete DNA sequence of yeast chromosome III

1992 • 851 citations

Regulation of p34cdc2 protein kinase during mitosis

1989 • 544 citations

Cell cycle-dependent specific positioning and clustering of centromeres and telomeres in fission yeast.

1993 • 506 citations

The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases

1989 • 486 citations

The NDA3 gene of fission yeast encodes β-tubulin: A cold-sensitive nda3 mutation reversibly blocks spindle formation and chromosome movement in mitosis

1984 • 473 citations

YAP1 dependent activation of TRX2 is essential for the response of Saccharomyces cerevisiae to oxidative stress by hydroperoxides.

1994 • 472 citations

Genetic engineering of Schizosaccharomyces pombe: A system for gene disruption and replacement using the ura4 gene as a selectable marker

1988 • 467 citations

Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization.

1984 • 444 citations

Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases.

1991 • 369 citations

High-frequency transformation of the fission yeast Schizosaccharomyces pombe

1981 • 351 citations

Yeast YAP1 encodes a novel form of the jun family of transcriptional activator proteins.

1989 • 328 citations

Higher order chromosome structure is affected by cold-sensitive mutations in a Schizosaccharomyces pombe gene crm1+ which encodes a 115-kD protein preferentially localized in the nucleus and its periphery.

1989 • 246 citations

Premature initiation of mitosis in yeast lacking RCC1 or an interacting GTPase

1991 • 231 citations

A temperature-sensitive mutation of the Schizosaccharomyces pombe gene nuc2+ that encodes a nuclear scaffold-like protein blocks spindle elongation in mitotic anaphase.

1988 • 220 citations

Metaphase Chromosome Structure: The Role of Nonhistone Proteins

1978 • 219 citations

Mitosis in the fission yeast Schizosaccharomyces pombe: a comparative study with light and electron microscopy

1971 • 218 citations

Identification of the pleiotropic cell division cycle gene NDA2 as one of two different α-tubulin genes in schizosaccharomyces pombe

1984 • 216 citations

The PAR1 (YAP1/SNQ3) gene of Saccharomyces cerevisiae, ac-jun homologue, is involved in oxygen metabolism

1992 • 209 citations

RCC1 in the cell cycle: the regulator of chromosome condensation takes on new roles

1993 • 192 citations

S. pombe gene sds22+ essential for a midmitotic transition encodes a leucine-rich repeat protein that positively modulates protein phosphatase-1

1991 • 191 citations

Interaction between cdc13+ and cdc2+ in the control of mitosis in fission yeast; dissociation of the G1 and G2 roles of the cdc2+ protein kinase.

1987 • 177 citations

The primary structure of the alcohol dehydrogenase gene from the fission yeast Schizosaccharomyces pombe.

1983 • 175 citations

Cold-sensitive nuclear division arrest mutants of the fission yeast Schizosaccharomyces pombe

1983 • 165 citations

A 13 kb resolution cosmid map of the 14 Mb fission yeast genome by nonrandom sequence-tagged site mapping

1993 • 150 citations

Sequential alterations in the nuclear chromatin region during mitosis of the fission yeast Schizosa Ccharomyces Pombe-: Video fluorescence microscopy of synchronously growing wild-type and cold-sensitive cdc mutants by using a dna-binding fluorescent probe

1981 • 135 citations

A fission yeast RCC1-related protein is required for the mitosis to interphase transition.

1994 • 134 citations

Retroviruses and insertional mutagenesis in mice: proviral integration at the Mov 34 locus leads to early embryonic death.

1987 • 133 citations

Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1.

1992 • 116 citations

Yeast chromosome III: new gene functions.

1994 • 104 citations

Temporal and spatial coordination of chromosome movement, spindle formation, and nuclear envelope breakdown during prometaphase in Drosophila melanogaster embryos.

1990 • 103 citations

Essential roles of the RNA polymerase I largest subunit and DNA topoisomerases in the formation of fission yeast nucleolus.

1989 • 84 citations

A stationary-phase protein of Escherichia coli that affects the mode of association between the trp repressor protein and operator-bearing DNA.

1993 • 81 citations

Fission yeast genes nda1+ and nda4+, mutations of which lead to S-phase block, chromatin alteration and Ca2+ suppression, are members of the CDC46/MCM2 family.

1993 • 78 citations

Characterization of PDR4, a Saccharomyces cerevisiae gene that confers pleiotropic drug resistance in high-copy number

1991 • 71 citations

Visualization of chromosomes in mitotically arrested cells of the fission yeast Schizosaccharomyces pombe

1983 • 70 citations

Schizosaccharomyces pombe Spk1 is a Tyrosine-Phosphorylated Protein Functionally Related to Xenopus Mitogen-Activated Protein Kinase

1993 • 57 citations

Schizosaccharomyces pombe Spk1 is a tyrosine-phosphorylated protein functionally related to Xenopus mitogen-activated protein kinase.

1993 • 44 citations

Molecular analysis of the Mov 34 mutation: transcript disrupted by proviral integration in mice is conserved in Drosophila

1990 • 42 citations

A data‐base of chromosome III of Saccharomyces cerevisiae

1993 • 37 citations

Isolation and sequence of two genes associated with a CpG island 5′ of the factor VIII gene

1992 • 26 citations

Higher-order chromosome structure in yeast

1990 • 20 citations

The complete sequence of a 10·8kb fragment to the right of the chromosome III centromere of Saccharomyces cerevisiae

1992 • 15 citations