NUP82 is an essential yeast nucleoporin required for poly(A)+ RNA export.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Improved tools for biological sequence comparison.

1988 • 11,455 citations

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

1989 • 8,705 citations

Predicting Coiled Coils from Protein Sequences

1991 • 3,977 citations

New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites

1988 • 2,903 citations

A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector

1987 • 1,181 citations

The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses

1987 • 850 citations

Identification and characterization of a nuclear pore complex protein

1986 • 610 citations

Correlation between structure and mass distribution of the nuclear pore complex and of distinct pore complex components.

1990 • 510 citations

Nup358, a Cytoplasmically Exposed Nucleoporin with Peptide Repeats, Ran-GTP Binding Sites, Zinc Fingers, a Cyclophilin A Homologous Domain, and a Leucine-rich Region

1995 • 471 citations

The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex

1995 • 460 citations

Generation of cDNA Probes Directed by Amino Acid Sequence: Cloning of Urate Oxidase

1988 • 325 citations

Internal protein sequence analysis: Enzymatic digestion for less than 10 μg of protein bound to polyvinylidene difluoride or nitrocellulose membranes

1992 • 290 citations

Isolation of the yeast nuclear pore complex.

1993 • 287 citations

Pores for thought: nuclear pore complex proteins

1994 • 286 citations

Structure of the leucine zipper

1992 • 282 citations

A new family of yeast nuclear pore complex proteins.

1992 • 256 citations

A novel nuclear pore protein Nup133p with distinct roles in poly(A)+ RNA transport and nuclear pore distribution.

1994 • 232 citations

The NUP1 gene encodes an essential component of the yeast nuclear pore complex

1990 • 219 citations

A temperature-sensitive NUP116 null mutant forms a nuclear envelope seal over the yeast nuclear pore complex thereby blocking nucleocytoplasmic traffic.

1993 • 211 citations

An Essential Yeast Gene Encoding a Homolog of Ubiquitin-activating Enzyme

1995 • 207 citations

A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes.

1995 • 192 citations

Purification of NSP1 reveals complex formation with ‘GLFG’ nucleoporins and a novel nuclear pore protein NIC96.

1993 • 189 citations

NSP1: A yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain

1990 • 180 citations

A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1.

1992 • 172 citations

Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p.

1995 • 167 citations

A novel nucleoskeletal-like protein located at the nuclear periphery is required for the life cycle of Saccharomyces cerevisiae.

1988 • 156 citations

Defects in mRNA 3'-end formation, transcription initiation, and mRNA transport associated with the yeast mutation prp20: possible coupling of mRNA processing and chromatin structure.

1992 • 142 citations

NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure.

1994 • 140 citations

The Essential Yeast Nucleoporin NUP159 Is Located on the Cytoplasmic Side of the Nuclear Pore Complex and Serves in Karyopherin-mediated Binding of Transport Substrate

1995 • 139 citations

Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif

1994 • 134 citations

NUP2, a novel yeast nucleoporin, has functional overlap with other proteins of the nuclear pore complex.

1993 • 119 citations

Mutation or deletion of the Saccharomyces cerevisiae RAT3/NUP133 gene causes temperature-dependent nuclear accumulation of poly(A)+ RNA and constitutive clustering of nuclear pore complexes.

1995 • 98 citations

Nuclear transport

1994 • 89 citations

Disruption of the nucleoporin gene NUP133 results in clustering of nuclear pore complexes.

1995 • 89 citations

Role of different domains in the self-association of rat nucleoporin p62

1994 • 51 citations