Comparison and optimization of in situ hybridization procedures yielding rapid, sensitive mRNA detections

Methods in Enzymology , Vol

1966 • 26,261 citations

A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity

1983 • 26,063 citations

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

1977 • 12,015 citations

PERIODATE-LYSINE-PARAFORMALDEHYDE FIXATIVE A NEW FIXATIVE FOR IMMUNOELECTRON MICROSCOPY

1974 • 3,882 citations

A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane

1965 • 1,981 citations

Methods of Enzymology.

1956 • 1,750 citations

Hybridization of nucleic acids immobilized on solid supports

1984 • 1,357 citations

Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.

1982 • 1,333 citations

Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

1981 • 1,165 citations

Genetic engineering. Principles and methods

1987 • 715 citations

Functional role for c-myc in mitogenic response to platelet-derived growth factor

1984 • 578 citations

Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin

1985 • 526 citations

Human β-globin pre-mRNA synthesized in vitro is accurately spliced in xenopus oocyte nuclei

1983 • 500 citations

Fluorescent phycobiliprotein conjugates for analyses of cells and molecules.

1982 • 455 citations

Application of immunofluorescent staining on paraffin sections improved by trypsin digestion.

1976 • 413 citations

An improved in situ hybridization method for the detection of cellular RNAs in Drosophila tissue sections and its application for localizing transcripts of the homeotic Antennapedia gene complex

1983 • 381 citations

Quantitative analysis ofin situhybridization methods for the detection of actin gene expression

1985 • 370 citations

Immunolabelling for electron microscopy

1983 • 335 citations

A colorimetric method for DNA hybridization

1984 • 292 citations

Texas red, a hydrophilic, red-emitting flourophore for use with flourescein in dual parameter flow microfluorometric and fluorescence microscopic studies

1982 • 270 citations

Bacteriophage SP6-specific RNA polymerase. I. Isolation and characterization of the enzyme.

1982 • 233 citations

Use of freeze-dried paraffin-embedded sections for immunohistologic staining with monoclonal antibodies.

1985 • 208 citations

The reaction of formaldehyde with nucleotides and T2 bacteriophage DNA

1961 • 160 citations

Isolation and identification of cross-links from formaldehyde-treated nucleic acids

1980 • 158 citations

Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization

1985 • 157 citations

Fluorescent tandem phycobiliprotein conjugates. Emission wavelength shifting by energy transfer

1983 • 133 citations

Efficiency of in situ hybridization as a function of probe size and fixation technique

1985 • 111 citations

Improved immunohistochemical localization of tissue antigens using modified methacarn fixation.

1985 • 108 citations

In situ hybridization of DNA sequences in human metaphase chromosomes visualized by an indirect fluorescent immunocytochemical procedure

1982 • 103 citations

Temporal Expression of Mouse Glial Fibrillary Acidic Protein mRNA Studied by a Rapid In Situ Hybridization Procedure

1985 • 101 citations

Quick-Blot: Selective mRNA or DNA Immobilization from Whole Cells

1983 • 97 citations

Quantitative in situ hybridization of ribosomal RNA species to polytene chromosomes of Drosophila melanogaster

1977 • 97 citations

The immunogold-silver staining method

1985 • 85 citations

A non-radioactivein situhybridization method based on mercurated nucleic acid probes and sulfhydryl-hapten ligands

1986 • 85 citations

Analysis of persistent virus infections by in situ hybridization to whole-mouse sections

1984 • 78 citations

Fixation of Thy-1 in nervous tissue for immunohistochemistry: a quantitative assessment of the effect of different fixation conditions upon retention of antigenicity and the cross-linking of Thy-1.

1983 • 75 citations

Detection of Two Viral Genomes in Single Cells by Double-Label Hybridization in Situ and Color Microradioautography

1985 • 72 citations

A double labeling technique for performing immunocytochemistry and in situ hybridization in virus infected cell cultures and tissues

1985 • 71 citations

In situ hybridization technique to localize rRNA and mRNA in mammalian neurons.

1986 • 71 citations

Biotin-Labeled Oligonucleotides: Enzymatic Synthesis and Use as Hybridization Probes

1984 • 68 citations

Bacteriophage SP6-specific RNA polymerase. II. Mapping of SP6 DNA and selective in vitro transcription.

1982 • 64 citations

Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligase

1984 • 59 citations

A single-step silver enhancement method permitting rapid diagnosis of cytomegalovirus infection in formalin-fixed, paraffin-embedded tissue sections by in situ hybridization and immunoperoxidase detection.

1986 • 58 citations

Immunofluorescence of deparaffinized, trypsin-treated renal tissues. Preservation of antigens as an adjunct to diagnosis of disease.

1979 • 56 citations

Specific detection of neuronal cell bodies: in situ hybridization with a biotin-labeled neurofilament cDNA probe.

1986 • 55 citations

Cytochemical hybridization with fluorochrome-labeled RNA. I. Development of a method using nucleic acids bound to agarose beads as a model.

1981 • 54 citations

Cellular levels of mRNA from c‐myc, c‐myb and c‐fes onc‐genes in normal myeloid anderythroid precursors of human bone marrow: an in situ hybridization study

1986 • 52 citations

Polynucleotide-histone H1 complexes as probes for blot hybridization.

1983 • 47 citations

Rapid and high resolution detection of in situ hybridisation to polytene chromosomes using fluorochrome-labeled RNA

1981 • 35 citations

Enhanced Mutability Associated with a Temperature-Sensitive Mutant of Vesicular Stomatitis Virus

1981 • 33 citations

A complex of single-strand binding protein and M13 DNA as hybridization probe

1985 • 32 citations

Immunocytochemical and enzyme histochemical localization of kallikrein-like enzymes in colon, intestine, and stomach of rat and cat.

1986 • 31 citations

Detection of AKR MuLV-specific RNA in AKR mouse cells by in situ hybridization

1979 • 31 citations

Immunofluorescence of Renal Lesions in Paraffinembedded and Fresh-frozen Sections

1980 • 29 citations

Tissue fixation with diimidoesters as an alternative to aldehydes. II. Cytochemical and biochemical studies of rat liver fixed with dimethylsuberimidate.

1976 • 26 citations

In situ hybridization: a routine method for parallel localization of DNA sequences and of their transcripts in consecutive paraffin sections with the use of 3H‐labelled nick translated cloned DNA probes

1985 • 25 citations

Virus-specific transcription and translation in organs of BALB / Mo mice: Comparative study using quantitative hybridization, in Situ hybridization, and immunocytochemistry

1982 • 20 citations

Alkali hydrolysis of RNA of Escherichia coli deposited on filter paper disks

1973 • 18 citations

Quantitativein SituHybridization of³H-Labeled Complementary Deoxyribonucleic Acid (cDNA) to the Messenger Ribonucleic Acid of Thyroglobulin in Human Thyroid Tissues*

1983 • 17 citations

A comparative study of in situ hybridization procedures using cRNA applied to Drosophila hydei salivary gland chromosomes

1974 • 17 citations

Glutaraldehyde nonfixation of isolated viral and yeast RNAs.

1980 • 14 citations

PHYCOBILIPROTEINS: COMPARISON OF SOLUTION AND SINGLE CRYSTAL FLUORESCENCE FOR C-PHYCOCYANIN AND B-PHYCOERYTHRIN

1982 • 8 citations

The study of fluorescent probes by quantitative video intensification microscopy (QVIM).

1986 • 7 citations

Metaplasia of the middle ear epithelium as studied with monoclonal anticytokeratin antibodies

1985 • 1 citations

Deleted Work

1955 • 0 citations