cDNA expression and human two‐dimensional gel protein databases: Towards integrating DNA and protein information

DNA cloning : a practical approach

1985 • 2,302 citations

An analysis of vertebrate mRNA sequences: intimations of translational control.

1991 • 1,655 citations

Cell biology :a laboratory handbook.

1994 • 1,213 citations

Vaccinia virus: a selectable eukaryotic cloning and expression vector.

1982 • 663 citations

Eukaryotic start and stop translation sites

1991 • 602 citations

Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus.

1982 • 506 citations

Vaccinia Virus: A Tool for Research and Vaccine Development

1991 • 410 citations

Molecular Cloning, Occurrence, and Expression of a Novel Partially Secreted Protein “Psoriasin” That Is Highly Up-Regulated in Psoriatic Skin

1991 • 410 citations

Escherichia coli gpt gene provides dominant selection for vaccinia virus open reading frame expression vectors

1988 • 317 citations

Characterization and primary structure of the poly(C)-binding heterogeneous nuclear ribonucleoprotein complex K protein.

1992 • 281 citations

A retinoblastoma-binding protein related to a negative regulator of Ras in yeast

1993 • 265 citations

Molecular Cloning and Expression of a Novel Keratinocyte Protein (Psoriasis-associated fatty acid-binding protein [PA-FABP]) that Is Highly Up-Regulated in Psoriatic Skin and that Shares Similarity to Fatty Acid-Binding Proteins

1992 • 257 citations

Characterisation of Two Major Cellular Poly(rC)-Binding Human Proteins, Each Containing Three K-homologous (KH) Domains

1995 • 205 citations

Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family.

1987 • 191 citations

Molecular Cloning and Expression of the Transformation Sensitive Epithelial Marker Stratifin

1993 • 177 citations

Tissue‐dependent variation in the expression of elongation factor‐1α isoforms: Isolation and characterisation of a cDNA encoding a novel variant of human elongation‐factor 1α

1993 • 160 citations

Molecular cloning and expression of a transformation-sensitive human protein containing the TPR motif and sharing identity to the stress-inducible yeast protein STI1.

1992 • 160 citations

One hundred base pairs of 5' flanking sequence of a vaccinia virus late gene are sufficient to temporally regulate late transcription.

1985 • 139 citations

Cloning and expression of a human 14-3-3 protein mediating phospholipolysis. Identification of an arachidonoyl-enzyme intermediate during catalysis.

1992 • 126 citations

Derepression of a novel class of vaccinia virus genes upon DNA replication.

1988 • 117 citations

Sequencing and expression of complementary DNA for the general transcription factor BTF3

1990 • 115 citations

The human keratinocyte two‐dimensional gel protein database (update 1995): Mapping components of signal transduction pathways

1995 • 115 citations

High expression of functional adenovirus DNA polymerase and precursor terminal protein using recombinant vaccinia virus

1988 • 94 citations

Human interferon gamma potently induces the synthesis of a 55-kDa protein (gamma 2) highly homologous to rabbit peptide chain release factor and bovine tryptophanyl-tRNA synthetase.

1991 • 94 citations

Identification, Molecular Cloning, Expression and Chromosome Mapping of a Family of Transformation Upregulated hnRNP-K Proteins Derived by Alternative Splicing

1994 • 91 citations

Identification of transformation sensitive proteins recorded in human two‐dimensional gel protein databases by mass spectrometric peptide mapping alone and in combination with microsequencing

1994 • 87 citations

Identification of Two Human Rho GDP Dissociation Inhibitor Proteins Whose Overexpression Leads to Disruption of the Actin Cytoskeleton

1993 • 86 citations

Expression and Cell Type—Specific Processing of Human Preproenkephalin with a Vaccinia Recombinant

1986 • 81 citations

Cloning and expression of a novel human profilin variant, profilin II

1993 • 80 citations

Human cellular protein patterns and their link to genome DNA sequence data: usefulness of two‐dimensional gel electrophoresis and microsequencing

1991 • 67 citations

Deacylation of the hemagglutinin of influenza A/Aichi/2/68 has no effect on membrane fusion properties

1991 • 66 citations

Microsequencing of proteins recorded in human two‐dimensional gel protein databases

1991 • 63 citations

Two‐dimensional gel electrophoresis, protein electroblotting and microsequencing: A direct link between proteins and genes

1990 • 60 citations

Cloning and expression of a cDNA covering the complete coding region of the P32 subunit of human pre-m RNA splicing factor SF2

1993 • 60 citations

Oxygen radical induced mutagenesis is DNA polymerase specific

1994 • 56 citations

Interferon‐γ up‐regulates a unique set of proteins in human keratinocytes

1993 • 55 citations

Mapping of Ras-related GTP-binding proteins by GTP overlay following two-dimensional gel electrophoresis.

1994 • 51 citations

Mapping of ras-related GTP-binding proteins by GTP-overlay and 2D-gel analysis.

1994 • 36 citations

Polylactosaminoglycan modification of the respiratory syncytial virus small hydrophobic (SH) protein: A conserved feature among human and bovine respiratory syncytial viruses

1992 • 34 citations

The molecular chaperones HSP28, GRP78, endoplasmin, and calnexin exhibit strikingly different levels in quiescent keratinocytes as compared to their proliferating normal and transformed counterparts: cDNA cloning and expression of calnexin

1994 • 26 citations

Role of N-linked glycans in antigenicity, processing, and cell surface expression of bovine herpesvirus 1 glycoprotein gIV

1993 • 24 citations

Assignment of the Human Genes Encoding 14-3-3 Eta (YWHAH) to 22q12, 14-3-3 Zeta (YWHAZ) to 2p25.1–p25.2, and 14-3-3 Beta (YWHAB) to 20q13.1 byin SituHybridization

1996 • 21 citations

Expression of cDNA clones by coupled in vitro transcription/translation and transfection into COS‐1 cells: Protein mapping in two‐dimensional gels

1995 • 8 citations

Cloning of a cDNA encoding a novel human nuclear phosphoprotein belonging to the WD-40 family

1994 • 8 citations

Expression of Recombinant Proteins in the Vaccinia Virus Expression System

1994 • 4 citations

UK health ann safety: £1,800 million bill

1979 • 2 citations