Vaccinia Virus: A Tool for Research and Vaccine Development

Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.

1986 • 2,118 citations

Smallpox and its eradication

1988 • 1,387 citations

The complete DNA sequence of vaccinia virus

1990 • 847 citations

HIV-specific cytotoxic T lymphocytes in seropositive individuals

1987 • 797 citations

Vaccinia virus expression vector: coexpression of beta-galactosidase provides visual screening of recombinant virus plaques.

1985 • 789 citations

Expression of cystic fibrosis transmembrane conductance regulator corrects defective chloride channel regulation in cystic fibrosis airway epithelial cells

1990 • 706 citations

Vaccinia virus: a selectable eukaryotic cloning and expression vector.

1982 • 663 citations

General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes

1984 • 652 citations

Influenza A virus nucleoprotein is a major target antigen for cross-reactive anti-influenza A virus cytotoxic T lymphocytes.

1985 • 526 citations

Differences in antigen presentation to MHC class I-and class II-restricted influenza virus-specific cytolytic T lymphocyte clones.

1986 • 513 citations

Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus.

1982 • 506 citations

HIV-1 gag-specific cytotoxic T lymphocytes defined with recombinant vaccinia virus and synthetic peptides

1988 • 447 citations

Decreased virulence of recombinant vaccinia virus expression vectors is associated with a thymidine kinase-negative phenotype

1985 • 447 citations

Infectious vaccinia virus recombinants that express hepatitis B virus surface antigen

1983 • 422 citations

Disseminated Vaccinia in a Military Recruit with Human Immunodeficiency Virus (HIV) Disease

1987 • 412 citations

Use of a hybrid vaccinia virus-T7 RNA polymerase system for expression of target genes.

1987 • 409 citations

Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5' sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system.

1989 • 401 citations

Protection from rabies by a vaccinia virus recombinant containing the rabies virus glycoprotein gene.

1984 • 379 citations

The Ick tyrosine protein kinase interacts with the cytoplasmic tail of the CD4 glycoprotein through its unique amino-terminal domain

1989 • 362 citations

Safety of and immunological response to a recombinant vaccinia virus vaccine expressing HIV envelope glycoprotein

1991 • 334 citations

Defective presentation to class I-restricted cytotoxic T lymphocytes in vaccinia-infected cells is overcome by enhanced degradation of antigen.

1988 • 331 citations

Escherichia coli gpt gene provides dominant selection for vaccinia virus open reading frame expression vectors

1988 • 317 citations

Infectious poxvirus vectors have capacity for at least 25 000 base pairs of foreign DNA

1983 • 311 citations

Live recombinant vaccinia virus protects chimpanzees against hepatitis B

1984 • 290 citations

Oligonucleotide sequence signaling transcriptional termination of vaccinia virus early genes.

1987 • 280 citations

Poxvirus pathogenesis

1991 • 273 citations

Vaccinia Virus Expression Vectors

1987 • 272 citations

HIV-1 Reverse Transcriptase Is a Target for Cytotoxic T Lymphocytes in Infected Individuals

1988 • 270 citations

Identification of viral molecules recognized by influenza-specific human cytotoxic T lymphocytes.

1987 • 251 citations

STUDIES ON SMALLPOX AND COMPLICATIONS OF SMALLPOX VACCINATION

1960 • 247 citations

Oral vaccination of the fox against rabies using a live recombinant vaccinia virus

1986 • 238 citations

Deletion of the vaccinia virus growth factor gene reduces virus virulence

1988 • 238 citations

Recombinant vaccinia virus primes and stimulates influenza haemagglutinin-specific cytotoxic T cells

1984 • 234 citations

Biological and immunological properties of human immunodeficiency virus type 1 envelope glycoprotein: analysis of proteins with truncations and deletions expressed by recombinant vaccinia viruses

1991 • 231 citations

Oral immunization and protection of raccoons (Procyon lotor) with a vaccinia-rabies glycoprotein recombinant virus vaccine.

1986 • 230 citations

Construction of live vaccines by using genetically engineered poxviruses: biological activity of recombinant vaccinia virus expressing influenza virus hemagglutinin.

1983 • 225 citations

Prevention of vaccinia virus infection in imiminodeficient mice by vector-directed IL-2 expression

1987 • 216 citations

Orthopoxvirus DNA: A comparison of restriction profiles and maps

1985 • 214 citations

Recombinant fowlpox virus inducing protective immunity in non-avian species

1988 • 209 citations

Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus

1983 • 206 citations

Recovery of immunodeficient mice from a vaccinia virus/IL-2 recombinant infection

1987 • 198 citations

Cytoplasmic expression system based on constitutive synthesis of bacteriophage T7 RNA polymerase in mammalian cells.

1990 • 195 citations

REGULATION OF VACCINIA VIRUS TRANSCRIPTION

1990 • 194 citations

Recombinant Vaccinia Virus: Immunization Against Multiple Pathogens

1985 • 192 citations

An endogenous processing pathway in vaccinia virus-infected cells for presentation of cytoplasmic antigens to class II-restricted T cells.

1990 • 188 citations

Human immunodeficiency virus envelope glycoprotein/CD4-mediated fusion of nonprimate cells with human cells

1990 • 185 citations

Removal of cryptic poxvirus transcription termination signals from the human immunodeficiency virus type 1 envelope gene enhances expression and immunogenicity of a recombinant vaccinia virus

1990 • 172 citations

Synthesis and cellular location of the ten influenza polypeptides individually expressed by recombinant vaccinia viruses

1987 • 166 citations

Mapping of the vaccinia virus thymidine kinase gene by marker rescue and by cell-free translation of selected mRNA

1982 • 162 citations

Cloning and expression of foreign genes in vaccinia virus, using a host range selection system

1989 • 151 citations

A nonstructural viral protein expressed by a recombinant vaccinia virus protects against lethal cytomegalovirus infection

1988 • 151 citations

Recognition of Influenza A Virus Nucleoprotein by Human Cytotoxic T Lymphocytes

1986 • 151 citations

Binding region for human immunodeficiency virus (HIV) and epitopes for HIV-blocking monoclonal antibodies of the CD4 molecule defined by site-directed mutagenesis.

1988 • 150 citations

Temporal regulation of influenza hemagglutinin expression in vaccinia virus recombinants and effects on the immune response

1986 • 149 citations

Protection of Cattle Against Rinderpest with Vaccinia Virus Recombinants Expressing the HA or F Gene

1988 • 142 citations

T-Lymphocyte Priming and Protection Against Friend Leukemia by Vaccinia-RetrovirusenvGene Recombinant

1986 • 141 citations

Mapping the fine specificity of a cytolytic T cell response to HIV-1 nef protein.

1990 • 133 citations

Structure and stability of mRNA synthesized by vaccinia virus-encoded bacteriophage T7 RNA polymerase in mammalian cells

1989 • 133 citations

A dominant selectable marker for the construction of recombinant poxviruses

1988 • 129 citations

Neomycin resistance as a dominant selectable marker for selection and isolation of vaccinia virus recombinants.

1985 • 128 citations

Genetic Engineering of Novel Genomes of Large DNA Viruses

1985 • 125 citations

Molecular genetics of vaccinia virus: demonstration of marker rescue.

1982 • 125 citations

Glycoprotein cytoplasmic domain sequences required for rescue of a vesicular stomatitis virus glycoprotein mutant

1989 • 124 citations

Anti‐viral protection and prevention of lymphocytic choriomeningitis or of the local footpad swelling reaction in mice by immunization with vaccinia‐recombinant virus expressing LCMV‐WE nucleoprotein or glycoprotein

1989 • 123 citations

Anti-influenza virus cytotoxic T lymphocytes recognize the three viral polymerases and a nonstructural protein: responsiveness to individual viral antigens is major histocompatibility complex controlled

1987 • 121 citations

New vaccinia virus recombination plasmids incorporating a synthetic late promoter for high level expression of foreign proteins

1990 • 119 citations

Immunodeficient mice recover from infection with vaccinia virus expressing interferon‐γ

1990 • 117 citations

Cytoplasmic transcription system encoded by vaccinia virus

1991 • 114 citations

Recognition of pre-processed endogenous antigen by class I but not class II MHC-restricted T cells

1989 • 113 citations

Interferon gamma is involved in the recovery of athymic nude mice from recombinant vaccinia virus/interleukin 2 infection.

1990 • 110 citations

Prevention of HIV-1 glycoprotein transport by soluble CD4 retained in the endoplasmic reticulum

1990 • 108 citations

Protection Against Streptococcal Pharyngeal Colonization with a Vaccinia: M Protein Recombinant

1989 • 105 citations

Mapping and insertional mutagenesis of a vaccinia virus gene encoding a 13,800-Da secreted protein

1989 • 104 citations

Elevated natural killer cell responses in mice infected with recombinant vaccinia virus encoding murine IL-2.

1990 • 103 citations

Tumour prevention and rejection with recombinant vaccinia

1987 • 101 citations

Passive transfer of respiratory syncytial virus (RSV) antiserum suppresses the immune response to the RSV fusion (F) and large (G) glycoproteins expressed by recombinant vaccinia viruses

1988 • 99 citations

Effective tumor immunotherapy directed against an oncogene-encoded product using a vaccinia virus vector.

1987 • 98 citations

Recombinant vaccinia virus vaccine against the human melanoma antigen p97 for use in immunotherapy.

1988 • 98 citations

Contribution of measles virus fusion protein in protective immunity: anti-F monoclonal antibodies neutralize virus infectivity and protect mice against challenge

1990 • 97 citations

Recombinant Vaccinia Viruses As Vectors for Studying T Lymphocyte Specificity and Function

1990 • 94 citations

Successful oral rabies vaccination of raccoons with raccoon poxvirus recombinants expressing rabies virus glycoprotein

1988 • 91 citations

Carboxy-terminally truncated dengue virus envelope glycoproteins expressed on the cell surface and secreted extracellularly exhibit increased immunogenicity in mice

1991 • 90 citations

Eukaryotic transient expression system dependent on transcription factors and regulatory DNA sequences of vaccinia virus.

1985 • 85 citations

Factor-dependent transcription termination by vaccinia virus RNA polymerase. Evidence that the cis-acting termination signal is in nascent RNA.

1988 • 84 citations

Vaccinia virus vectors utilizing the β-galactosidase assay for rapid selection of recombinant viruses and measurement of gene expression

1986 • 84 citations

Regulated expression of foreign genes in mammalian cells under the control of coliphage T3 RNA polymerase and lac repressor.

1989 • 83 citations

The Roles of Influenza Virus Haemagglutinin and Nueleoprotein in Protection: Analysis Using Vaccinia Virus Recombinants

1987 • 82 citations

Recognition of cloned influenza virus hemagglutinin gene products by cytotoxic T lymphocytes

1986 • 79 citations

Genetic studies with mammalian poxviruses

1958 • 78 citations

Cells that express all five proteins of vesicular stomatitis virus from cloned cDNAs support replication, assembly, and budding of defective interfering particles.

1991 • 72 citations

A poxvirus-derived vector that directs high levels of expression of cloned genes in mammalian cells.

1988 • 69 citations

Insertional inactivation of the large subunit of ribonucleotide reductase encoded by vaccinia virus is associated with reduced virulence in vivo

1990 • 69 citations

Large-Scale Production and Purification of a Vaccinia Recombinant-Derived HIV-1 gp160 and Analysis of Its Immunogenicity

1989 • 65 citations

Effects and virulences of recombinant vaccinia viruses derived from attenuated strains that express the human T-cell leukemia virus type I envelope gene

1988 • 62 citations

Class I MHC can present an endogenous peptide to cytotoxic T lymphocytes.

1989 • 62 citations

The Origins of Vaccinia Virus

1977 • 61 citations

Anchoring a Secreted Plasmodium Antigen on the Surface of Recombinant Vaccinia Virus-Infected Cells Increases Its Immunogenicity

1986 • 60 citations

Heterologously expressed serotonin 1A receptors couple to muscarinic K+ channels in heart.

1991 • 59 citations

[34] Vaccinia virus as an expression vector

1987 • 55 citations

High level gene expression in mammalian cells by a nuclear T7-phage RNA polymerase

1989 • 54 citations

The Enzymology of Poxvirus DNA Replication

1990 • 52 citations

Reverse guanine phosphoribosyltransferase selection of recombinant vaccinia viruses

1990 • 52 citations

A 15 amino acid fragment of influenza nucleoprotein synthesized in the cytoplasm is presented to class I-restricted cytotoxic T lymphocytes.

1989 • 51 citations

Other Orthopoxviruses

1989 • 49 citations

Anchor sequence-dependent endogenous processing of human immunodeficiency virus 1 envelope glycoprotein gp160 for CD4+ T cell recognition.

1990 • 49 citations

The Molecular Pathogenesis of Poxviruses

1990 • 48 citations

Successful vaccination with a polyvalent live vector despite existing immunity to an expressed antigen

1988 • 44 citations

The immunogenicity of VP7, a rotavirus antigen resident in the endoplasmic reticulum, is enhanced by cell surface expression

1990 • 38 citations

Attenuation and immunogenicity in primates of vaccinia virus recombinants expressing human interleukin-2

1990 • 37 citations

Expression of polyomavirus virion proteins by a vaccinia virus vector: association of VP1 and VP2 with the nuclear framework

1987 • 37 citations

Effectiveness of enteric immunization in the development of secretory immunoglobulin A response and the outcome of infection with respiratory syncytial virus

1991 • 37 citations

The Role of Telomeres in Poxvirus DNA Replication

1990 • 36 citations

Epitope mapping by deletion mutants and chimeras of two vesicular stomatitis virus glycoprotein genes expressed by a vaccinia virus vector

1989 • 36 citations

Plaque size phenotype as a selectable marker to generate vaccinia virus recombinants

1989 • 32 citations

Synthesis of Functional mRNA in Mammalian Cells by Bacteriophage T3 RNA Polymerase

1990 • 32 citations

Expression of the M gene of vesicular stomatitis virus cloned in various vaccinia virus vectors

1988 • 29 citations

Regulated expression of nuclear genes by T3 RNA polymerase and lac repressor, using recombinant vaccinia virus vectors

1990 • 27 citations

Methisazone in Progressive Vaccinia

1967 • 26 citations

Transport to the cell surface of a peptide sequence attached to the truncated C terminus of an N-terminally anchored integral membrane protein.

1988 • 25 citations

Posttranslational Modification of Vaccinia Virus Proteins

1990 • 25 citations

Expression of ion channels and receptors in Xenopus oocytes using vaccinia virus

1991 • 21 citations

Response of monkeys to vaccination with recombinant vaccinia virus which coexpress HIV gpl60 and human interleukin‐2

1990 • 20 citations

A Novel Approach for the Production of Monoclonal Antibodies Using Infectious Vaccinia Virus Recombinants

1987 • 19 citations

Antigen-presenting Capacity of Epidermal Cells Infected with Vaccinia Virus Recombinants Containing the Herpes Simplex Virus Glycoprotein D, and Protective Immunity

1989 • 17 citations

RECIPROCITY IN THE INTERACTIONS BETWEEN THE POXVIRUSES AND THEIR HOST CELLS

1990 • 12 citations

Classics of Medicine and Surgery

1961 • 12 citations

Orthopoxvirus gene expression in Xenopus laevis oocytes: a component of the virion is needed for late gene expression

1990 • 4 citations