Differential Structural Requirements for Interaction of Ras Protein with Its Distinct Downstream Effectors

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

1985 • 9,128 citations

Transformation of intact yeast cells treated with alkali cations

1983 • 7,688 citations

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

1988 • 6,107 citations

Mammalian Ras interacts directly with the serine/threonine kinase raf

1993 • 1,983 citations

Phosphatidylinositol-3-OH kinase direct target of Ras

1994 • 1,923 citations

Refined crystal structure of the triphosphate conformation of H-ras p21 at 1.35 A resolution: implications for the mechanism of GTP hydrolysis.

1990 • 1,077 citations

Molecular Switch for Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic ras Proteins

1990 • 1,037 citations

Complexes of Ras⋅GTP with Raf-1 and Mitogen-Activated Protein Kinase Kinase

1993 • 987 citations

A ras-related gene with transformation suppressor activity

1989 • 979 citations

Normal and oncogenic p21ras proteins bind to the amino-terminal regulatory domain of c-Raf-1

1993 • 876 citations

nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine.

1990 • 859 citations

Direct interaction of Ras and the amino-terminal region of Raf-1 in vitro

1993 • 730 citations

Multiple ras functions can contribute to mammalian cell transformation

1995 • 658 citations

Complex formation between RAS and RAF and other protein kinases.

1993 • 633 citations

The 2.2 Å crystal structure of the Ras-binding domain of the serine/threonine kinase c-Raf1 in complex with RaplA and a GTP analogue

1995 • 627 citations

Raf meets Ras: completing the framework of a signal transduction pathway

1994 • 591 citations

The ins and outs of Raf kinases

1994 • 537 citations

High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs bytrans-complementation ofSchizosaccharomyces pombe

1990 • 434 citations

DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase

1985 • 429 citations

Three-dimensional structures of H-ras p21 mutants: Molecular basis for their inability to function as signal switch molecules

1990 • 395 citations

Cellular interactions in development : a practical approach

1993 • 373 citations

Human cDNAs rap1 and rap2 homologous to the Drosophila gene Dras3 encode proteins closely related to ras in the 'effector' region.

1988 • 334 citations

Identification of effector residues and a neutralizing epitope of Ha-ras-encoded p21.

1986 • 291 citations

Activated Ras interacts with the Ral guanine nucleotide dissociation stimulator.

1994 • 281 citations

Role of a ras homolog in the life cycle of schizosaccharomyces pombe

1986 • 274 citations

Identification of the guanine nucleotide dissociation stimulator for Ral as a putative effector molecule of R-ras, H-ras, K-ras, and Rap.

1994 • 270 citations

Premeiotic DNA synthesis in fission yeast

1974 • 262 citations

ralGDS family members interact with the effector loop of ras p21.

1994 • 260 citations

A conserved kinase cascade for MAP kinase activation in yeast

1993 • 255 citations

Two Distinct Raf Domains Mediate Interaction with Ras

1995 • 243 citations

Evidence for the in vitro and in vivo interaction of Ras with protein kinase C zeta.

1994 • 221 citations

byr2, a Schizosaccharomyces pombe gene encoding a protein kinase capable of partial suppression of the ras1 mutant phenotype.

1991 • 208 citations

Mutational analysis of a ras catalytic domain.

1986 • 205 citations

The effector interactions of p21ras

1993 • 193 citations

The ras oncogene--an important regulatory element in lower eucaryotic organisms

1989 • 189 citations

GTP-dependent association of Raf-1 with Ha-Ras: identification of Raf as a target downstream of Ras in mammalian cells.

1993 • 180 citations

Critical binding and regulatory interactions between Ras and Raf occur through a small, stable N-terminal domain of Raf and specific Ras effector residues.

1994 • 175 citations

Cysteine-rich Region of Raf-1 Interacts with Activator Domain of Post-translationally Modified Ha-Ras

1995 • 157 citations

Involvement of ras in sexual differentiation but not in growth control in fission yeast

1986 • 139 citations

The Effect of Posttranslational Modifications on the Interaction of Ras2 with Adenylyl Cyclase

1993 • 139 citations

Leucine-rich repeats and carboxyl terminus are required for interaction of yeast adenylate cyclase with RAS proteins.

1990 • 133 citations

Association of MEK1 with p21ras.GMPPNP is dependent on B-Raf.

1994 • 73 citations

Protein Kinase Byr2 Is a Target of Ras1 in the Fission Yeast Schizosaccharomyces pombe

1995 • 70 citations

The post-translational modification of ras p21 is important for Raf-1 activation.

1994 • 69 citations

Mutations that abolish the ability of Ha-Ras to associate with Raf-1.

1994 • 58 citations

Identification of discrete segments of human Raf-1 kinase critical for high affinity binding to Ha-Ras.

1994 • 55 citations

Asparagine 26, glutamic acid 31, valine 45, and tyrosine 64 of Ras proteins are required for their oncogenicity.

1992 • 54 citations

Identification of amino acid residues required for Ras p21 target activation.

1991 • 53 citations

Raf-1 N-Terminal Sequences Necessary for Ras-Raf Interaction and Signal Transduction

1995 • 50 citations

p21-ras effector domain mutants constructed by "cassette" mutagenesis.

1988 • 47 citations

Quantitative analysis of mutually competitive binding of human Raf-1 and yeast adenylyl cyclase to Ras proteins.

1994 • 44 citations

Conformation change of effector-region residues in antiparallel β-sheet of human c-Ha-ras protein on GDP→GTPγS exchange: A two-dimensional NMR study

1989 • 30 citations

A glutamic acid residue at position 31 of Ras protein is essential to the signal transduction for neurite outgrowth of PC12 cells and the stimulation of GTPase activity by GAPRas.

1992 • 29 citations

Site-directed mutagenesis, fluorescence, and two-dimensional NMR studies on microenvironments of effector region aromatic residues of human c-Ha-Ras protein

1994 • 27 citations

A Constitutive Effector Region on the C-terminal Side of Switch I of the Ras Protein

1995 • 25 citations

Identification of amino acid residues of Ras protein that are essential for signal‐transducing activity but not for enhancement of GTPase activity by GAP

1991 • 13 citations