A putative Ca2+-binding protein: structure of the light subunit of porcine calpain elucidated by molecular cloning and protein sequence analysis.

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

1979 • 22,482 citations

[57] Sequencing end-labeled DNA with base-specific chemical cleavages

1980 • 15,302 citations

Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose

1972 • 6,922 citations

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

1982 • 6,153 citations

Prediction of the Secondary Structure of Proteins from their Amino Acid Sequence

1979 • 2,930 citations

Amino acid analysis by reverse-phase high-performance liquid chromatography: Precolumn derivatization with phenylisothiocyanate

1984 • 1,616 citations

High-Efficiency Cloning of Full-Length cDNA

1982 • 1,309 citations

[47] Establishing homologies in protein sequences

1983 • 1,187 citations

Plasmid screening at high colony density

1980 • 1,100 citations

Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue

1983 • 1,028 citations

Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin

1982 • 928 citations

Calcium-binding proteins and calcium function

1977 • 742 citations

[36] High-sensitivity sequencing with a gas-phase sequenator

1983 • 670 citations

Purification and characterization of a unique isozyme of cytochrome P-450 from liver microsomes of ethanol-treated rabbits.

1982 • 537 citations

Separation of large denatured peptides by reverse phase high performance liquid chromatography. Trifluoroacetic acid as a peptide solvent.

1980 • 376 citations

Troponin and Parvalbumin Calcium Binding Regions Predicted in Myosin Light Chain and T4 Lysozyme

1975 • 374 citations

Intracellular Ca2+-dependent protease (CALPAIN) and its high-molecular-weight endogenous inhibitor (CALPASTATIN)

1981 • 374 citations

[43] Analysis of phenylthiohydantoins by ultrasensitive gradient high-performance liquid chromatography

1983 • 358 citations

Evolutionary origin of a calcium-dependent protease by fusion of genes for a thiol protease and a calcium-binding protein?

1984 • 337 citations

Mouse beta 2-microglobulin cDNA clones: a screening procedure for cDNA clones corresponding to rare mRNAs.

1981 • 320 citations

Canine cardiac calcium‐dependent proteases: Resolution of two forms with different requirements for calcium

1980 • 267 citations

Calcium and Cell Function

1984 • 178 citations

Solid Phase Methods in Protein Sequence Analysis

1978 • 133 citations

A general method for detection and characterization of an mRNA using an oligonucleotide probe.

1981 • 128 citations

Proteases: potential role in health and disease.

1984 • 82 citations

The Chou‐Fasman secondary structure prediction method with an extended data base

1978 • 69 citations

Sequence of the amino-terminal 349 residues of rabbit muscle glycogen phosphorylase including the sites of covalent and allosteric control

1978 • 49 citations

Correspondence of homologies in amino acid sequence and tertiary structure of protein molecules

1982 • 49 citations

Studies on the Ca2+-Activated Neutral Proteinase of Rabbit Skeletal Muscle. I. The Characterization of the 80 K and the 30 K Subunits

1981 • 49 citations

Large-scale purification of porcine calpain I and calpain II and comparison of proteolytic fragments of their subunits.

1984 • 45 citations

Effect of Metal Ions on the Structure and Activity of Calcium-Activated Neutral Protease (CANP)1

1983 • 32 citations

Reconstitution of calpain I and calpain II from their subunits: Interchangeability of the light subunits

1984 • 30 citations