Future medical applications of single-cell sequencing in cancer

Hallmarks of Cancer: The Next Generation

2011 • 58,651 citations

Real-Time DNA Sequencing from Single Polymerase Molecules

2008 • 3,446 citations

Isolation of rare circulating tumour cells in cancer patients by microchip technology

2007 • 3,431 citations

mRNA-Seq whole-transcriptome analysis of a single cell

2009 • 3,180 citations

Tumour evolution inferred by single-cell sequencing

2011 • 2,478 citations

Distant metastasis occurs late during the genetic evolution of pancreatic cancer

2010 • 2,359 citations

Tumor Cells Circulate in the Peripheral Blood of All Major Carcinomas but not in Healthy Subjects or Patients With Nonmalignant Diseases

2004 • 2,355 citations

Cancer as an evolutionary and ecological process

2006 • 1,658 citations

Comprehensive human genome amplification using multiple displacement amplification

2002 • 1,429 citations

Molecular Definition of Breast Tumor Heterogeneity

2007 • 1,338 citations

Genome remodelling in a basal-like breast cancer metastasis and xenograft

2010 • 1,138 citations

Mutational evolution in a lobular breast tumour profiled at single nucleotide resolution

2009 • 1,060 citations

Whole genome amplification from a single cell: implications for genetic analysis.

1992 • 898 citations

Lumpectomy Compared with Lumpectomy and Radiation Therapy for the Treatment of Intraductal Breast Cancer

1993 • 832 citations

Complex landscapes of somatic rearrangement in human breast cancer genomes

2009 • 828 citations

Personalized copy number and segmental duplication maps using next-generation sequencing

2009 • 730 citations

I-SPY 2: An Adaptive Breast Cancer Trial Design in the Setting of Neoadjuvant Chemotherapy

2009 • 707 citations

Genetic clonal diversity predicts progression to esophageal adenocarcinoma

2006 • 692 citations

Sensitive and accurate detection of copy number variants using read depth of coverage

2009 • 604 citations

Tracing the Derivation of Embryonic Stem Cells from the Inner Cell Mass by Single-Cell RNA-Seq Analysis

2010 • 508 citations

Prevention of invasive breast cancer in women with ductal carcinoma in situ: An update of the National Surgical Adjuvant Breast and Bowel Project experience

2001 • 504 citations

High-resolution mapping of copy-number alterations with massively parallel sequencing

2008 • 500 citations

Inferring tumor progression from genomic heterogeneity

2009 • 493 citations

[Radiotherapy in breast-conserving treatment for ductal carcinoma in situ: first results of the EORTC randomised phase III trial 10853].

2001 • 459 citations

Comparative genomic hybridization, loss of heterozygosity, and DNA sequence analysis of single cells

1999 • 449 citations

How Darwinian models inform therapeutic failure initiated by clonal heterogeneity in cancer medicine

2010 • 406 citations

Biomarker Expression and Risk of Subsequent Tumors After Initial Ductal Carcinoma In Situ Diagnosis

2010 • 337 citations

Cellular and genetic diversity in the progression of in situ human breast carcinomas to an invasive phenotype

2010 • 326 citations

High-resolution aCGH and expression profiling identifies a novel genomic subtype of ER negative breast cancer

2007 • 322 citations

Novel patterns of genome rearrangement and their association with survival in breast cancer

2006 • 319 citations

Mechanism of chimera formation during the Multiple Displacement Amplification reaction

2007 • 285 citations

Ductal Carcinoma In situ and the Emergence of Diversity during Breast Cancer Evolution

2008 • 279 citations

Predictive and Prognostic Value of Peripheral Blood Cytokeratin-19 mRNA-Positive Cells Detected by Real-Time Polymerase Chain Reaction in Node-Negative Breast Cancer Patients

2006 • 267 citations

Whole Genome Analysis of Genetic Alterations in Small DNA Samples Using Hyperbranched Strand Displacement Amplification and Array–CGH

2003 • 267 citations

Characteristics Associated With Recurrence Among Women With Ductal Carcinoma In Situ Treated by Lumpectomy

2003 • 237 citations

Prognostic Value of the Molecular Detection of Circulating Tumor Cells Using a Multimarker Reverse Transcription-PCR Assay for Cytokeratin 19, Mammaglobin A, and HER2 in Early Breast Cancer

2008 • 232 citations

Single circulating tumor cell detection and overall survival in nonmetastatic breast cancer

2009 • 231 citations

Genetic Heterogeneity in HER2 Testing in Breast Cancer: Panel Summary and Guidelines

2009 • 227 citations

Chromosome analysis of 97 primary breast carcinomas: Identification of eight karyotypic subgroups

1995 • 203 citations

Direct Genetic Analysis of Single Disseminated Cancer Cells for Prediction of Outcome and Therapy Selection in Esophageal Cancer

2008 • 203 citations

HE4: a new potential early biomarker for the recurrence of ovarian cancer

2010 • 178 citations

Single-Cell PCR Analysis of TCR Repertoires Selected by Antigen In Vivo: A High Magnitude CD8 Response Is Comprised of Very Few Clones

1996 • 174 citations

DNA Sudoku—harnessing high-throughput sequencing for multiplexed specimen analysis

2009 • 168 citations

Intratumor genomic heterogeneity in breast cancer with clonal divergence between primary carcinomas and lymph node metastases

2006 • 167 citations

Genomic analysis of genetic heterogeneity and evolution in high-grade serous ovarian carcinoma

2010 • 165 citations

Gene Expression Profiling in Cervical Cancer: An Exploration of Intratumor Heterogeneity

2006 • 149 citations

High resolution array-CGH analysis of single cells

2006 • 149 citations

The genetics of cancer—a 3D model

1999 • 141 citations

Intratumoral Heterogeneity in Breast Carcinoma Revealed by Laser-Microdissection and Comparative Genomic Hybridization

1999 • 123 citations

Circulating melanoma cells and survival in metastatic melanoma

2011 • 112 citations

New technologies for the detection of circulating tumour cells

2010 • 111 citations

Using next-generation sequencing for high resolution multiplex analysis of copy number variation from nanogram quantities of DNA from formalin-fixed paraffin-embedded specimens

2010 • 110 citations

Identification of small gains and losses in single cells after whole genome amplification on tiling oligo arrays

2009 • 106 citations

Novel genomic alterations and clonal evolution in chronic lymphocytic leukemia revealed by representational oligonucleotide microarray analysis (ROMA)

2008 • 103 citations

CNAseg—a novel framework for identification of copy number changes in cancer from second-generation sequencing data

2010 • 103 citations

Genotypic Intratumoral Heterogeneity in Breast Carcinoma With HER2/neu Amplification

2009 • 101 citations

Detection and Removal of Biases in the Analysis of Next-Generation Sequencing Reads

2011 • 99 citations

Karyotypic comparisons of multiple tumorous and macroscopically normal surrounding tissue samples from patients with breast cancer.

1996 • 98 citations

Breast cancer assessment tools and optimizing adjuvant therapy

2010 • 89 citations

Intratumoral Heterogeneity of HER2/neu in Breast Cancer?A Rare Event

2007 • 88 citations

PSA and Beyond: The Past, Present, and Future of Investigative Biomarkers for Prostate Cancer

2010 • 84 citations

Clonal heterogeneity in breast cancer: Karyotypic comparisons of multiple intra—and extra—tumorous samples from 3 patients

1995 • 82 citations

Approaching Solid Tumor Heterogeneity on a Cellular Basis by Tissue Proteomics Using Laser Capture Microdissection and Biological Mass Spectrometry

2009 • 80 citations

ERCC5 Is a Novel Biomarker of Ovarian Cancer Prognosis

2008 • 76 citations

Bone marrow micrometastasis in breast cancer: review of detection methods, prognostic impact and biological issues

2007 • 75 citations

Intra-tumour genetic heterogeneity and poor chemoradiotherapy response in cervical cancer

2010 • 71 citations

Cytogenetic and flow cytometry DNA analysis of regional heterogeneity in a low grade human glioma.

1995 • 67 citations

Detection of CK-19 mRNA-positive cells in the peripheral blood of breast cancer patients with histologically and immunohistochemically negative axillary lymph nodes

2005 • 61 citations

High-resolution array comparative genomic hybridization of single micrometastatic tumor cells

2008 • 59 citations

Evaluation of Phi29-based whole-genome amplification for microarray-based comparative genomic hybridisation

2006 • 56 citations

Clone heterogeneity in diploid and aneuploid breast carcinomas as detected by FISH

2001 • 54 citations

Interphase cytogenetics reveals a high incidence of aneuploidy and intra-tumour heterogeneity in breast cancer

1995 • 45 citations

Molecular analysis of multifocal prostate cancer by comparative genomic hybridization

2008 • 42 citations

Heterogeneity of chromosome 17 and erbB‐2 gene copy number in primary and metastatic bladder cancer

1995 • 42 citations

Evaluation and significance of circulating epithelial cells in patients with hormone‐refractory prostate cancer

2007 • 41 citations

Tumor heterogeneity in lung cancer based on light microscopic features

1983 • 37 citations

Genetic heterogeneity and clonal evolution in neuroblastoma

2001 • 35 citations

Oncogenic role of p53 is suppressed by si-RNA bicistronic construct of uPA, uPAR and cathepsin-B in meningiomas both in vitro and in vivo

2011 • 34 citations

Single-Cell PCR Analysis of T Helper Cells in Human Lymph Node Germinal Centers

2000 • 33 citations

Chromosomal imbalances in primary and metastatic pancreatic carcinoma as detected by interphase cytogenetics: basic findings and clinical aspects

1998 • 31 citations

Flowcytometry ‐ Principles and Feasibility in Transfusion Medicine. Enumeration of Epithelial Derived Tumor Cells in Peripheral Blood

1998 • 31 citations

Aneuploidy of chromosome 8 as detected by interphase fluorescence in situ hybridization is a recurrent finding in primary and metastatic breast cancer

1998 • 28 citations

Use of whole genome amplification and comparative genomic hybridisation to detect chromosomal copy number alterations in cell line material and tumour tissue

2004 • 27 citations

Intratumoral nuclear morphologic heterogeneity in prostate cancer

1997 • 25 citations

Whole genome amplification and its impact on CGH array profiles

2008 • 24 citations

Prognostic significance of a grading system considering tumor heterogeneity in muscle-invasive urothelial carcinoma of the urinary bladder

2003 • 24 citations

Molecular Analysis of the Immunoglobulin VH Gene Rearrangement in a Primary Cutaneous Immunoblastic B-Cell Lymphoma by Micromanipulation and Single-Cell PCR

1997 • 23 citations

Cytogenetic manifestations of multiple myeloma heterogeneity

2004 • 22 citations

Intratumoral genetic heterogeneity in pilocytic astrocytomas revealed by CGH-analysis of microdissected tumor cells and FISH on tumor tissue sections

2006 • 20 citations

Whole Genome Amplification for Array Comparative Genomic Hybridization Using DNA Extracted from Formalin-Fixed, Paraffin-Embedded Histological Sections

2009 • 19 citations

Cytogenetic investigation of testicular carcinoma in situ and early seminoma by high-resolution comparative genomic hybridization analysis of subpopulations flow sorted according to DNA content

2004 • 15 citations

Homogeneity and heterogeneity in pancreas cancer: presence of predominant and minor morphological types and implications

1986 • 12 citations

Bar-Coded, Multiplexed Sequencing of Targeted DNA Regions Using the Illumina Genome Analyzer

2010 • 6 citations