Characterization of plasma membranes from A431 cells, isolated by self-generating Percoll gradient: a rapid isolation procedure to obtain plasma membranes with functional epidermal growth factor receptors

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

1951 • 319,299 citations

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION

1959 • 47,127 citations

Inositol trisphosphate, a novel second messenger in cellular signal transduction

1984 • 6,088 citations

Epidermal Growth Factor

1979 • 2,964 citations

G proteins and dual control of adenylate cyclase

1984 • 1,811 citations

Quantitative analysis of phospholipids by thin-layer chromatography

1964 • 1,414 citations

Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes.

1980 • 1,184 citations

Role of guanine nucleotide binding protein in the activation of polyphosphoinositide phosphodiesterase

1985 • 960 citations

A rapid and sensitive sub-micro phosphorus determination

1961 • 951 citations

Epidermal growth factor-receptor-protein kinase interactions. Co-purification of receptor and epidermal growth factor-enhanced phosphorylation activity.

1980 • 853 citations

A native 170,000 epidermal growth factor receptor-kinase complex from shed plasma membrane vesicles.

1982 • 788 citations

Common elements in growth factor stimulation and oncogenic transformation: 85 kd phosphoprotein and phosphatidylinositol kinase activity

1987 • 670 citations

Rapid enhancement of protein phosphorylation in A-431 cell membrane preparations by epidermal growth factor.

1979 • 530 citations

Epidermal growth factor stimulates phosphorylation in membrane preparations in vitro

1978 • 448 citations

5-Hydroxytryptamine stimulates inositol phosphate production in a cell-free system from blowfly salivary glands. Evidence for a role of GTP in coupling receptor activation to phosphoinositide breakdown.

1985 • 429 citations

Enhancement of calcium uptake and phosphatidylinositol turnover by epidermal growth factor in A-431 cells

1981 • 426 citations

Isolation of a calcium-dependent 35-kilodalton substrate for the epidermal growth factor receptor/kinase from A-431 cells.

1984 • 366 citations

Chemoattractant receptor-induced hydrolysis of phosphatidylinositol 4,5-bisphosphate in human polymorphonuclear leukocyte membranes. Requirement for a guanine nucleotide regulatory protein.

1985 • 358 citations

Rapid isolation of plasma membranes in high yield from cultured fibroblasts

1977 • 304 citations

The epidermal growth factor-induced calcium signal in A431 cells.

1986 • 297 citations

Action of highly purified phospholipases on blood platelets. Evidence for an asymmetric distribution of phospholipids in the surface membrane

1977 • 248 citations

Epidermal growth factor stimulates the production of phosphatidylinositol monophosphate and the breakdown of polyphosphoinositides in A431 cells.

1987 • 230 citations

Epidermal growth factor stimulates the rapid accumulation of inositol (1,4,5)-trisphosphate and a rise in cytosolic calcium mobilized from intracellular stores in A431 cells.

1987 • 202 citations

Thyrotropin-releasing hormone activates a Ca2+-dependent polyphosphoinositide phosphodiesterase in permeable GH3 cells. GTP gamma S potentiation by a cholera and pertussis toxin-insensitive mechanism.

1986 • 197 citations

Epidermal growth factor, its receptor, and related proteins

1986 • 186 citations

Evidence suggesting that a novel guanine nucleotide regulatory protein couples receptors to phospholipase C in exocrine pancreas

1986 • 177 citations

The protein-tyrosine kinase substrate, p81, is homologous to a chicken microvillar core protein.

1986 • 173 citations

Characterization by electrophoresis of epidermal growth factor stimulated phosphorylation using A-431 membranes

1980 • 153 citations

Epidermal growth factor stimulates the phosphorylation of the calcium-dependent 35,000-dalton substrate in intact A-431 cells.

1985 • 149 citations

Asymmetric distribution of arachidonic acid in the plasma membrane of human platelets A determination using purified phospholipases and a rapid method for membrane isolation

1979 • 144 citations

Activation of 45Ca2+ influx and 22Na+/H+ exchange by epidermal growth factor and vanadate in A431 cells is independent of phosphatidylinositol turnover and is inhibited by phorbol ester and diacylglycerol.

1986 • 101 citations

Epidermal growth factor (EGF) stimulates inositol trisphosphate formation in cells which overexpress the EGF receptor

1987 • 99 citations

Lipids of ocular tissues. I. The phospholipids of mature rabbit and bovine lens.

1969 • 89 citations

Lipids of ocular tissues

1969 • 81 citations

Effect of 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) on the growth inhibitory and increased phosphatidylinositol (PI) responses induced by epidermal growth factor (EGF) in A431 cells

1983 • 71 citations

Biochemical characterization of plasma membranes and intracellular membranes isolated from human platelets using Percoll gradients

1986 • 56 citations

Rapid and efficient purification of plasma membrane from cultured cells: characterization of epidermal growth factor binding

1987 • 55 citations

Isolation and characterization of plasma membranes from Krebs II ascite cells using percoll gradient

1982 • 55 citations

Studies on enzymes related to diacylglycerol production in activated platelets II. Subcellular distribution, enzymatic properties and positional specificity of diacylglycerol- and monoacylglycerol-lipases

1984 • 44 citations

A rapid isolation procedure of plasma membranes from human neutrophils using self-generating Percoll gradients. Importance of pH in avoiding contamination by intracellular membranes

1985 • 38 citations

[41] Purification of the receptor for epidermal growth factor from A-431 cells: its function as a tyrosyl kinase

1983 • 35 citations

Subcellular localization of inositol lipids in blood platelets as deduced from the use of labelled precursors

1987 • 27 citations