Designing and optimizing library selection strategies for generating high-affinity antibodies

Phage antibodies: filamentous phage displaying antibody variable domains

1990 • 2,346 citations

By-passing immunization

1991 • 1,603 citations

Making Antibodies by Phage Display Technology

1994 • 1,516 citations

Canonical structures for the hypervariable regions of immunoglobulins

1987 • 1,357 citations

Organ targeting In vivo using phage display peptide libraries

1996 • 1,198 citations

Making antibody fragments using phage display libraries

1991 • 1,195 citations

Human Antibodies with Sub-nanomolar Affinities Isolated from a Large Non-immunized Phage Display Library

1996 • 1,021 citations

Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains

1991 • 1,014 citations

Isolation of high affinity human antibodies directly from large synthetic repertoires.

1994 • 970 citations

Man-made antibodies

1991 • 935 citations

Antibody fragments from a ‘single pot’ phage display library as immunochemical reagents.

1994 • 578 citations

Human anti-self antibodies with high specificity from phage display libraries.

1993 • 480 citations

By-passing immunisation

1992 • 434 citations

CDR Walking Mutagenesis for the Affinity Maturation of a Potent Human Anti-HIV-1 Antibody into the Picomolar Range

1995 • 331 citations

Isolation of High-affinity Monomeric Human Anti-c-erbB-2 Single chain Fv Using Affinity-driven Selection

1996 • 295 citations

Molecular Profile of an Antibody Response to HIV-1 as Probed by Combinatorial Libraries

1993 • 229 citations

The Imprint of Somatic Hypermutation on the Repertoire of Human Germline V Genes

1996 • 201 citations

Phage libraries for generation of clinically useful antibodies

1994 • 179 citations

In-cell PCR from mRNA: amplifying and linking the rearranged immunoglobulin heavy and light chain V-genes within single cells

1992 • 133 citations

Affinity Maturation of a High-affinity Human Monoclonal Antibody Against the Third Hypervariable Loop of Human Immunodeficiency Virus: Use of Phage Display to Improve Affinity and Broaden Strain Reactivity

1996 • 108 citations

Strategies for expressing human antibody repertoires in transgenic mice

1996 • 94 citations

In Vitro Assembly of Repertoires of Antibody Chains on the Surface of Phage by Renaturation

1994 • 79 citations

Identification of functional and structural amino-acid residues by parsimonious mutagenesis

1996 • 62 citations

Selection of an anti-IGF-1 Fab from a Fab phage library created by mutagenesis of multiple CDR loops

1993 • 55 citations

Cell selection strategies for making antibodies from variable gene libraries: trapping the memory pool

1992 • 54 citations