Conserved Extracellular Cysteine Pair in the M₃ Muscarinic Acetylcholine Receptor Is Essential for Proper Receptor Cell Surface Localization but Not for G Protein Coupling

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction

1973 • 12,522 citations

“Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A

1981 • 8,657 citations

LIGAND: A versatile computerized approach for characterization of ligand-binding systems

1980 • 8,534 citations

Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides

1983 • 1,937 citations

Identification of a Family of Muscarinic Acetylcholine Receptor Genes

1987 • 1,439 citations

STRUCTURE AND FUNCTION OF G PROTEIN-COUPLED RECEPTORS

1994 • 1,070 citations

[71] Use of eukaryotic expression technology in the functional analysis of cloned genes

1987 • 834 citations

Protein degradation in the endoplasmic reticulum

1990 • 659 citations

Antagonist binding profiles of five cloned human muscarinic receptor subtypes.

1991 • 605 citations

[35] Epitope tagging and protein surveillance

1991 • 526 citations

Functional heterogeneity of mutant rhodopsins responsible for autosomal dominant retinitis pigmentosa.

1991 • 492 citations

Molecular Biology of Muscarinic Acetylcholine Receptors

1996 • 488 citations

Cysteine residues 110 and 187 are essential for the formation of correct structure in bovine rhodopsin.

1988 • 409 citations

Structural features required for ligand binding to the beta-adrenergic receptor.

1987 • 358 citations

Assembly of functional rhodopsin requires a disulfide bond between cysteine residues 110 and 187.

1990 • 320 citations

Using PCR to Engineer DNA

1989 • 303 citations

The G-Protein Linked Receptor Facts Book

1994 • 292 citations

Role of extracellular disulfide-bonded cysteines in the ligand binding function of the .beta.2-adrenergic receptor

1990 • 215 citations

Plasma Membrane Localization and Functional Rescue of Truncated Forms of a G Protein-coupled Receptor

1995 • 158 citations

Functional rescue of mutant V2 vasopressin receptors causing nephrogenic diabetes insipidus by a co-expressed receptor polypeptide.

1996 • 152 citations

Propylbenzilylcholine Mustard Labels an Acidic Residue in Transmembrane Helix 3 of the Muscarinic Receptor

1989 • 146 citations

Muscarinic acetylcholine receptors. Peptide sequencing identifies residues involved in antagonist binding and disulfide bond formation.

1990 • 138 citations

Site-directed mutagenesis of the m2 muscarinic acetylcholine receptor. Analysis of the role of N-glycosylation in receptor expression and function.

1990 • 134 citations

Hm1 muscarinic cholinergic receptor internalization requires a domain in the third cytoplasmic loop.

1992 • 129 citations

Structure and function in rhodopsin: replacement by alanine of cysteine residues 110 and 187, components of a conserved disulfide bond in rhodopsin, affects the light-activated metarhodopsin II state.

1994 • 126 citations

Site-directed mutagenesis of the rat m1 muscarinic acetylcholine receptor. Role of conserved cysteines in receptor function.

1992 • 126 citations

The high affinity state of the beta 2-adrenergic receptor requires unique interaction between conserved and non-conserved extracellular loop cysteines.

1994 • 123 citations

Assembly, sorting, and exit of oligomeric proteins from the endoplasmic reticulum

1998 • 108 citations

Functional Role of the Third Cytoplasmic Loop in Muscarinic Receptor Dimerization

1996 • 98 citations

Binding-, intracellular transport-, and biosynthesis-defective mutants of vasopressin type 2 receptor in patients with X-linked nephrogenic diabetes insipidus.

1995 • 85 citations

Deletion analysis of the mouse m1 muscarinic acetylcholine receptor: effects on phosphoinositide metabolism and down-regulation

1989 • 77 citations

A Disulfide Bond between Conserved Extracellular Cysteines in the Thyrotropin-releasing Hormone Receptor Is Critical for Binding

1995 • 76 citations

Charged amino acids required for signal transduction by the m3 muscarinic acetylcholine receptor.

1993 • 67 citations

The peptide binding site of the substance P (NK-1) receptor localized by a photoreactive analogue of substance P: presence of a disulfide bond.

1996 • 62 citations

An X-Linked NDI Mutation Reveals a Requirement for Cell Surface V2R Expression

1997 • 53 citations

Internalization of the Hm1 muscarinic cholinergic receptor involves the third cytoplasmic loop

1990 • 52 citations

Role of cysteine residues in the extracellular domain and exoplasmic loops of the transmembrane domain of the TSH receptor: Effect of mutation to serine on TSH receptor activity and response to thyroid stimulating autoantibodies

1992 • 48 citations

A disulfide bonding interaction role for cysteines in the extracellular domain of the thyrotropin-releasing hormone receptor.

1996 • 28 citations

Quality control in protein biogenesis: Thiol-mediated retention monitors the redox state of proteins in the endoplasmic reticulum

1996 • 26 citations

Interaction of deglycosylate muscarinic receptors with ligands and G proteins

1990 • 18 citations

Point Mutations of the Thyrotropin Receptor Determining Structural Requirements for Its Ability to Bind Thyrotropin and to Stimulate Adenylate Cyclase Activity

1994 • 9 citations

A disulfide bonding interaction role for cysteines in the extracellular domain of the thyrotropin-releasing hormone receptor

1996 • 9 citations