Isolation and Characterization of MC3T3-E1 Preosteoblast Subclones with Distinct In Vitro and In Vivo Differentiation/Mineralization Potential

Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction

1987 • 64,270 citations

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

1987 • 45,673 citations

Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

1980 • 8,711 citations

Osf2/Cbfa1: A Transcriptional Activator of Osteoblast Differentiation

1997 • 4,191 citations

Targeted Disruption of Results in a Complete Lack of Bone Formation owing to Maturational Arrest of Osteoblasts

1997 • 4,168 citations

The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid

1961 • 3,860 citations

Cbfa1, a Candidate Gene for Cleidocranial Dysplasia Syndrome, Is Essential for Osteoblast Differentiation and Bone Development

1997 • 2,784 citations

In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria.

1983 • 1,661 citations

Progressive development of the rat osteoblast phenotype in vitro: Reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix

1990 • 1,546 citations

Mutations Involving the Transcription Factor CBFA1 Cause Cleidocranial Dysplasia

1997 • 1,463 citations

Expression cloning of a common receptor for parathyroid hormone and parathyroid hormone-related peptide from rat osteoblast-like cells: a single receptor stimulates intracellular accumulation of both cAMP and inositol trisphosphates and increases intracellular free calcium.

1992 • 1,009 citations

Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture: An in vitro model of osteoblast development

1992 • 949 citations

Relationship of cell growth to the regulation of tissue‐specific gene expression during osteoblast differentiation

1990 • 934 citations

Mineralized bone nodules formedin vitro from enzymatically released rat calvaria cell populations

1986 • 855 citations

Single-Colony Derived Strains of Human Marrow Stromal Fibroblasts Form Bone After Transplantation In Vivo

1997 • 722 citations

Nucleation of hydroxyapatite by bone sialoprotein.

1993 • 657 citations

PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila runt gene and the human AML1 gene.

1993 • 621 citations

Two Distinct Osteoblast-Specific cis-Acting Elements Control Expression of a Mouse Osteocalcin Gene

1995 • 564 citations

Relationship between collagen synthesis and expression of the osteoblast phenotype in MC3T3-E1 cells

1992 • 538 citations

Factors that promote progressive development of the osteoblast phenotype in cultured fetal rat calvaria cells

1990 • 515 citations

BONE FORMATION IN VIVO: COMPARISON OF OSTEOGENESIS BY TRANSPLANTED MOUSE AND HUMAN MARROW STROMAL FIBROBLASTS

1997 • 488 citations

Antisera and cDNA probes to human and certain animal model bone matrix noncollagenous proteins

1995 • 452 citations

Runt homology domain proteins in osteoblast differentiation: AML3/CBFA1 is a major component of a bone-specific complex

1997 • 441 citations

Effects of ascorbic acid on collagen matrix formation and osteoblast differentiation in murine MC3T3-E1 cells

1994 • 431 citations

Modulation of crystal formation by bone phosphoproteins: structural specificity of the osteopontin-mediated inhibition of hydroxyapatite formation

1994 • 427 citations

Expression of bone sialoprotein (BSP) in developing human tissues

1991 • 407 citations

Isolation of the human gene for bone gla protein utilizing mouse and rat cDNA clones.

1986 • 398 citations

Expression of differentiated function by mineralizing cultures of chicken osteoblasts

1987 • 397 citations

Developmental expression of bone sialoprotein mRNA in rat mineralized connective tissues

1992 • 393 citations

Role of the α2-Integrin in Osteoblast-specific Gene Expression and Activation of the Osf2 Transcription Factor

1998 • 373 citations

Modulation of crystal formation by bone phosphoproteins: role of glutamic acid-rich sequences in the nucleation of hydroxyapatite by bone sialoprotein

1994 • 349 citations

Physiological Concentrations of Glucocorticoids Stimulate Formation of Bone Nodules from Isolated Rat Calvaria Cellsin Vitro

1987 • 311 citations

Differentiation and Cell Surface Expression of Transforming Growth Factor-β Receptors Are Regulated by Interaction with Matrix Collagen in Murine Osteoblastic Cells

1996 • 203 citations

Coordinate regulation of the levels of type III and type I collagen mRNA in most but not all mouse fibroblasts.

1985 • 194 citations

Ascorbic Acid-Dependent Activation of the Osteocalcin Promoter in MC3T3-E1 Preosteoblasts: Requirement for Collagen Matrix Synthesis and the Presence of an Intact OSE2 Sequence

1997 • 187 citations

Reduction in Transforming Growth Factor β Receptor I Expression and Transcription Factor CBFa1 on Bone Cells by Glucocorticoid

1998 • 160 citations

Osteopontin and Related Phosphorylated Sialoproteins: Effects on Mineralization^a

1995 • 153 citations

Bone matrix mRNA expression in differentiating fetal bovine osteoblasts

1992 • 141 citations

Bone morphogenetic protein-2b stimulation of growth and osteogenic phenotypes in rat osteoblast-like cells: Comparison with TGF-β1

1991 • 129 citations

Bone sialoprotein in developing porcine dental tissues: Cellular expression and comparison of tissue localization with osteopontin and osteonectin

1993 • 127 citations

The Mature Osteoblast Phenotype Is Characterized by Extensive Plasticity

1997 • 114 citations

Bone sialoprotein is localized to the root surface during cementogenesis

1994 • 106 citations

Murine bone sialoprotein (BSP): cDNA cloning, mRNA expression, and genetic mapping

1994 • 96 citations

Differential effects and glucocorticoid potentiation of bone morphogenetic protein action during rat osteoblast differentiation in vitro.

1996 • 94 citations

Distribution and synthesis of bone sialoprotein in metaphyseal bone of young rats show a distinctly different pattern from that of osteopontin.

1994 • 84 citations

Mineralization of bone-like extracellular matrix in the absence of functional osteoblasts

1995 • 78 citations

Fibronectin gene expression, synthesis, and accumulation during in vitro differentiation of chicken osteoblasts

1995 • 74 citations

Isolation and characterization of the mouse liver/bone/kidney-type alkaline phosphatase gene

1990 • 70 citations

Ribosomal DNA of the fly Sciara coprophila has a very small and homogeneous repeat unit

1979 • 69 citations

Subclone heterogeneity in a clonally-derived osteoblast-like cell line

1985 • 48 citations

Phenotypic Heterogeneity of Osteoblast-like MC3T3-E1 Cells: Changes of Bradykinin-Induced Prostaglandin E2 Production During Osteoblast Maturation

1997 • 36 citations

Regulation and Properties of Bone Alkaline Phosphatase during Vitamin C Deficiency in Guinea Pigs

1996 • 34 citations

Alteration of Bone Status with Ascorbic Acid Deficiency in ODS (Osteogenic Disorder Shionogi) Rats

1995 • 29 citations

Bone sialoprotein knockout mice have bone abnormalities

1995 • 19 citations

A study of collagen gene expression in normally and abnormally healing human fractures

1995 • 3 citations