Preparation and characterization of a disulfide-stabilized Fv fragment of the anti-Tac antibody: Comparison with its single-chain analog

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

1986 • 6,284 citations

The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Application to the radioimmunoassay

1973 • 3,279 citations

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli.

1988 • 1,536 citations

Single-Chain Antigen-Binding Proteins

1988 • 1,450 citations

A monoclonal antibody (anti-Tac) reactive with activated and functionally mature human T cells. I. Production of anti-Tac monoclonal antibody and distribution of Tac (+) cells.

1981 • 1,366 citations

Rapid tumor penetration of a single-chain Fv and comparison with other immunoglobulin forms.

1992 • 824 citations

A comparison of strategies to stabilize immunoglobulin Fv-fragments

1990 • 469 citations

A recombinant immunotoxin consisting of two antibody variable domains fused to Pseudomonas exotoxin

1989 • 420 citations

A method for increasing the yield of properly folded recombinant fusion proteins: Single-chain immunotoxins from renaturation of bacterial inclusion bodies

1992 • 343 citations

Recombinant Toxins for Cancer Treatment

1991 • 308 citations

An improved linker for single-chain Fv with reduced aggregation and enhanced proteolytic stability

1993 • 289 citations

The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA

1985 • 247 citations

A recombinant immunotoxin containing a disulfide-stabilized Fv fragment.

1993 • 226 citations

Anti-Tac-H, a humanized antibody to the interleukin 2 receptor with new features for immunotherapy in malignant and immune disorders.

1990 • 150 citations

Conformational stability, folding, and ligand-binding affinity of single-chain Fv immunoglobulin fragments expressed in Escherichia coli

1991 • 145 citations

Characterization of monoclonal antibodies B1 and B3 that react with mucinous adenocarcinomas.

1991 • 140 citations

Recombinant immunotoxins containing anti-Tac(Fv) and derivatives of Pseudomonas exotoxin produce complete regression in mice of an interleukin-2 receptor-expressing human carcinoma

1994 • 137 citations

Stabilization of the Fv fragments in recombinant immunotoxins by disulfide bonds engineered into conserved framework regions

1994 • 128 citations

Engineering interchain disulfide bonds into conserved framework regions of Fv fragments: improved biochemical characteristics of recombinant immunotoxins containing disulfide-stabilized Fv

1994 • 113 citations

Anti-Tac(Fv)-PE40, a single chain antibody Pseudomonas fusion protein directed at interleukin 2 receptor bearing cells.

1990 • 94 citations

Clinical radioimmunodetection, 1978-1988: overview and suggestions for standardization of clinical trials.

1990 • 72 citations

[IMMUNITY TO CANCER].

1964 • 46 citations

Pharmacokinetic considerations on monoclonal antibodies.

1989 • 27 citations

Radioimmunodetection of solid tumors. Future horizons and applications for radioimmunotherapy

1993 • 20 citations

The coming of age of cancer radioimmunoconjugates

1993 • 19 citations

Radioantibodies for the Diagnosis and Treatment of Cancer; Radioimmunoimaging (RAI) and Radioimmunotherapy (RAT)

1990 • 8 citations