Selective Inactivation of the Exonuclease Activity of Bacteriophage T7 DNA Polymerase by in Vitro Mutagenesis

A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

1976 • 225,752 citations

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

1976 • 200,127 citations

Rapid and efficient site-specific mutagenesis without phenotypic selection.

1985 • 7,573 citations

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

1985 • 3,185 citations

Empirical Predictions of Protein Conformation

1978 • 3,167 citations

The catalytic decomposition of hydrogen peroxide by iron salts

1934 • 2,483 citations

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

1987 • 2,266 citations

Chemical Modification of Proteins

2012 • 1,097 citations

Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP

1985 • 855 citations

Chemical Modification of Proteins

1974 • 419 citations

Escherichia coli thioredoxin confers processivity on the DNA polymerase activity of the gene 5 protein of bacteriophage T7.

1987 • 399 citations

Genetic and Crystallographic Studies of the 3′,5′-Exonucleolytic Site of DNA Polymerase I

1988 • 344 citations

Cocrystal structure of an editing complex of Klenow fragment with DNA.

1988 • 324 citations

Helix-random coil transitions in DNA homopolymer pairs

1964 • 289 citations

Nonenzymatic cleavage of proteins by reactive oxygen species generated by dithiothreitol and iron.

1985 • 268 citations

Escherichia coli thioredoxin: a subunit of bacteriophage T7 DNA polymerase.

1976 • 267 citations

Escherichia coli thioredoxin stabilizes complexes of bacteriophage T7 DNA polymerase and primed templates.

1987 • 207 citations

Photooxidation with Rose Bengal of a Critical Histidine Residue in Yeast Enolase^*

1965 • 193 citations

Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.

1983 • 168 citations

Interaction of mutant thioredoxins of Escherichia coli with the gene 5 protein of phage T7. The redox capacity of thioredoxin is not required for stimulation of DNA polymerase activity.

1986 • 146 citations

Exonucleolytic proofreading

1988 • 133 citations

Deoxyribonucleic Acid Polymerase of Bacteriophage T7

1971 • 132 citations

Domain of E. coli DNA polymerase I showing sequence homology to T7 DNA polymerase

1985 • 118 citations

Enzymatic Synthesis of Deoxyribonucleic Acid

1969 • 117 citations

A preformed, topologically stable replication fork. Characterization of leading strand DNA synthesis catalyzed by T7 DNA polymerase and T7 gene 4 protein.

1983 • 113 citations

Bacteriophage T7 deoxyribonucleic acid replication invitro. Bacteriophage T7 DNA polymerase: an an emzyme composed of phage- and host-specific subunits.

1975 • 111 citations

Bacteriophage T7 Deoxyribonucleic acid replication in vitro. A protein of Escherichia coli required for bacteriophage T7 DNA polymerase activity.

1975 • 100 citations

Characterization of strand displacement synthesis catalyzed by bacteriophage T7 DNA polymerase.

1983 • 100 citations

The speEspeD operon of Escherichia coli. Formation and processing of a proenzyme form of S-adenosylmethionine decarboxylase.

1987 • 90 citations

Control of mutation frequency by bacteriophage T4 DNA polymerase. II. Accuracy of nucleotide selection by the L88 mutator, CB120 antimutator, and wild type phage T4 DNA polymerases.

1976 • 82 citations

The effect of the T7 and Escherichia coli DNA-binding proteins at the replication fork of bacteriophage T7.

1988 • 82 citations

Two forms of the DNA polymerase of bacteriophage T7.

1983 • 81 citations

Leading and lagging strand synthesis at the replication fork of bacteriophage T7. Distinct properties of T7 gene 4 protein as a helicase and primase.

1988 • 71 citations

Mixed-function oxidation of histidine residues

1984 • 70 citations

Deoxyribonucleic acid polymerase of bacteriophage T7. Characterization of the exonuclease activities of the gene 5 protein and the reconstituted polymerase.

1979 • 67 citations

A DNA Polymerase Induced by Bacteriophage T7

1971 • 61 citations

Selective oxidation of the exonuclease domain of bacteriophage T7 DNA polymerase.

1987 • 56 citations

T7-induced DNA polymerase. Characterization of associated exonuclease activities and resolution into biologically active subunits.

1979 • 55 citations

Bacteriophage T7 DNA replication in vitro. Stimulation of DNA synthesis by T7 RNA polymerase.

1980 • 49 citations

Vit C.Fe(III) induced loss of the covalently bound phosphate and enzyme activity of phosphoglucomutase.

1985 • 27 citations