Human hemoglobin expression in Escherichia coli: importance of optimal codon usage

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

The codon adaptation index-a measure of directional synonymous codon usage bias, and its potential applications

1987 • 3,628 citations

Improved free-energy parameters for predictions of RNA duplex stability.

1986 • 1,472 citations

Correlation between the abundance of Escherichia coli transfer RNAs and the occurrence of the respective codons in its protein genes: A proposal for a synonymous codon choice that is optimal for the E. coli translational system

1981 • 1,405 citations

The crystal structure of human deoxyhaemoglobin at 1.74 Å resolution

1984 • 738 citations

X-ray Diffraction Study of Binding of 2,3-Diphosphoglycerate to Human Deoxyhaemoglobin

1972 • 731 citations

ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification

1988 • 673 citations

Structure of human oxyhaemoglobin at 2·1resolution

1983 • 618 citations

Codon usage patterns inEscherichia coli, Bacillus subtilis, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Drosophila melanogasterandHomo sapiens; a review of the considerable within-species diversity

1988 • 584 citations

Codon usage determines translation rate in Escherichia coli

1989 • 577 citations

[29] Synthesis and sequence-specific proteolysis of hybrid proteins produced in Escherichia coli

1987 • 392 citations

Generation of β-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli

1984 • 374 citations

Codon frequencies in 119 individual genes confirm corsistent choices of degenerate bases according to genome type

1980 • 370 citations

Preparation and Properties of α- and β-Chains from Human Hemoglobin

1969 • 363 citations

High-level expression of sperm whale myoglobin in Escherichia coli.

1987 • 338 citations

The role of the distal histidine in myoglobin and haemoglobin

1988 • 288 citations

The effects of amino acid substitution at position E7 (residue 64) on the kinetics of ligand binding to sperm whale myoglobin.

1990 • 288 citations

The structure of human carbonmonoxy haemoglobin at 2.7 Å resolution

1980 • 271 citations

Expression of fully functional tetrameric human hemoglobin in Escherichia coli.

1990 • 234 citations

Isolation and DNA sequence of a full-length cDNA clone for human X chromosome-encoded phosphoglycerate kinase.

1983 • 232 citations

Nucleotide sequence of the Pseudomonas putida cytochrome P-450cam gene and its expression in Escherichia coli.

1986 • 225 citations

Characterization of T7-specific Ribonucleic Acid Polymerase

1974 • 180 citations

Distal residues in the oxygen binding site of haemoglobin studied by protein engineering

1987 • 160 citations

Synthesis, bacterial expression, and mutagenesis of the gene coding for mammalian cytochrome b5.

1986 • 151 citations

X-ray diffraction and solution studies of specifically carbamylated human hemoglobin A. Evidence for the location of a proton- and oxygen-linked chloride binding site at valine 1 alpha.

1979 • 128 citations

Oxygenation-linked subunit interactions in human hemoglobin: analysis of linkage functions for constituent energy terms

1976 • 119 citations

Influence of Prosthetic Groups on Protein Folding and Subunit Assembly

1972 • 118 citations

Cloning, expression in Escherichia coli, and reconstitution of human myoglobin.

1985 • 111 citations

The role of Val68(E11) in ligand binding to sperm whale myoglobin. Site-directed mutagenesis of a synthetic gene.

1990 • 104 citations

Nuclear magnetic resonance quadrupole relaxation studies of chloride binding to human oxy- and deoxyhaemoglobin

1972 • 104 citations

Genetic analysis of the folding pathway for the tail spike protein of phage P22.

1983 • 103 citations

Experimental resolution of cooperative free energies for the ten ligation states of human hemoglobin.

1985 • 93 citations

The organization and complete nucleotide sequence of the PstI restriction-modification system.

1984 • 74 citations

Oxygen-linked binding sites for inorganic anions to hemoglobin.

1980 • 70 citations

Structure-specific model of hemoglobin cooperativity.

1983 • 67 citations

Design, synthesis and expression of a human interteukin-2 gene incorporating the codon usage bias found in highly expressed Escherichia coli genes

1988 • 67 citations

Functional role of the distal valine (E11) residue of α subunits in human haemoglobin

1991 • 48 citations

Site-directed mutagenesis in haemoglobin

1991 • 47 citations

Structure of haemoglobin in the deoxy quaternary state with ligand bound at the α haems

1989 • 45 citations

RNA primary sequence or secondary structure in the translational initiation region controls expression of two variant interferon-beta genes in Escherichia coli.

1990 • 37 citations

Recombinant human hemoglobin: Expression and refolding of β-globin fromEscherichia coli

1991 • 34 citations

Variations in codon usage are not correlated with heterologous gene expression in Saccharomyces cerevisiae and Escherichia coli

1988 • 27 citations

Experimental resolution of cooperative free energies for the ten ligation species of cobalt(II)/iron(II)-carbon monoxide hemoglobin

1991 • 26 citations

Effect of the distal residues on the vibrational modes of the iron-carbon monoxide bond in hemoglobin studied by protein engineering

1990 • 24 citations

In vitro characterization of hybrid promoters and altered tryptophan operon promoters

1985 • 4 citations