Self-sustained sequence replication (3SR): an isothermal transcription-based amplification system alternative to PCR.

Molecular Cloning: A Laboratory Manual

2001 • 133,517 citations

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

PCR protocols — A guide to methods and applications

1990 • 12,897 citations

Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

1985 • 9,128 citations

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

1987 • 5,745 citations

Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates

1987 • 2,098 citations

Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements

1983 • 1,316 citations

Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA

1990 • 1,270 citations

Effects of primer-template mismatches on the polymerase chain reaction: Human immunodeficiency virus type 1 model studies

1990 • 1,037 citations

Genetic disease detection and DNA amplification using cloned thermostable ligase.

1991 • 803 citations

Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication.

1990 • 527 citations

Novel method for studying mRNA phenotypes in single or small numbers of cells

1989 • 382 citations

Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene from Thermus aquaticus

1989 • 370 citations

PCR Technology

1989 • 297 citations

The ligation amplification reaction (LAR)—Amplification of specific DNA sequences using sequential rounds of template-dependent ligation

1989 • 258 citations

Processivity in early stages of transcription by T7 RNA polymerase

1988 • 256 citations

Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format.

1989 • 253 citations

Nearest Neighbor Influences on DNA Polymerase Insertion Fidelity

1989 • 247 citations

Isolation and Characterization of an Endonuclease from Escherichia coli Specific for Ribonucleic Acid in Ribonucleic Acid·Deoxyribonucleic Acid Hybrid Structures

1973 • 223 citations

Characterization of T7-specific Ribonucleic Acid Polymerase

1973 • 204 citations

The ligase chain reaction in a PCR world.

1991 • 202 citations

A computer program for selection of oligonucleotide primers for polymerase chain reactions

1990 • 195 citations

Characterization of T7-specific Ribonucleic Acid Polymerase

1974 • 180 citations

Sandwich hybridization as a convenient method for the detection of nucleic acids in crude samples

1983 • 172 citations

Physical Chemistry

1978 • 140 citations

Kinetic analysis of T7 RNA polymerase-promoter interactions with small synthetic promoters

1987 • 134 citations

Endonuclease activity of purified RNA-directed DNA polymerase from avian myeloblastosis virus.

1979 • 100 citations

The synthesis of high yields of full-length reverse transcripts of globin mRNA

1977 • 94 citations

Optimization strategies for the polymerase chain reaction.

1989 • 94 citations

Sequence and analysis of the gene for bacteriophage T3 RNA polymerase

1985 • 86 citations

Theoretical biology and biophysics

1971 • 82 citations

Thermophilic DNA ligase. Purification and properties of the enzyme from Thermus thermophilus HB8.

1984 • 79 citations

Use of inorganic pyrophosphatase to improve the yield of in vitro transcription reactions catalyzed by T7 RNA polymerase.

1990 • 69 citations

[15a] Purification and detection of reverse transcriptase in viruses and cells

1974 • 59 citations

Sequences of three promoters for the bacteriophage SP6 RNA polymerase

1986 • 58 citations

Dot blot detection of point mutations with adjacently hybridising synthetic oligonucleotide probes

1988 • 51 citations

Mechanism of Action of the Endonuclease Associated with the αβ and ββ Forms of Avian RNA Tumor Virus Reverse Transcriptase

1983 • 47 citations

Relationship between promoter structure and template specificities exhibited by the bacteriophage T3 and T7 RNA polymerases.

1983 • 46 citations

Detection of Human Immunodeficiency Virus Type 1 in AIDS Patients Using Amplification-Mediated Hybridization Analyses: Reproducibility and Quantitative Limitations

1990 • 37 citations

Use of avian myeloblastosis virus reverse transcriptase at high temperature for sequence analysis of highly structured RNA

1989 • 29 citations

Differential inhibition of DNA polymerase and RNase H activities of the reverse transcriptase by phosphonoformate

1982 • 23 citations

Unique features of the self-sustained sequence replication (3SR) reaction in the in vitro amplification of nucleic acids.

1990 • 22 citations

RNA sequencing using fluorescent-labeled dideoxynucleotides and automated fluorescence detection

1990 • 17 citations