Genetic disease detection and DNA amplification using cloned thermostable ligase.

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

1988 • 17,059 citations

Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms.

1989 • 3,707 citations

A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

1985 • 3,185 citations

Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification

1988 • 1,260 citations

Multiple Mutations in HIV-1 Reverse Transcriptase Confer High-Level Resistance to Zidovudine (AZT)

1989 • 1,199 citations

Effects of primer-template mismatches on the polymerase chain reaction: Human immunodeficiency virus type 1 model studies

1990 • 1,037 citations

Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes.

1989 • 1,025 citations

A Ligase-Mediated Gene Detection Technique

1988 • 834 citations

Reactivity of cytosine and thymine in single-base-pair mismatches with hydroxylamine and osmium tetroxide and its application to the study of mutations.

1988 • 636 citations

Detection of Single Base Substitutions by Ribonuclease Cleavage at Mismatches in RNA:DNA Duplexes

1985 • 538 citations

Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides.

1983 • 536 citations

Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication.

1990 • 527 citations

DNA Diagnostics—Molecular Techniques and Automation

1988 • 286 citations

DNA fingerprinting on trial

1989 • 285 citations

The ligation amplification reaction (LAR)—Amplification of specific DNA sequences using sequential rounds of template-dependent ligation

1989 • 258 citations

Single-sperm typing: determination of genetic distance between the G gamma-globin and parathyroid hormone loci by using the polymerase chain reaction and allele-specific oligomers.

1989 • 195 citations

Detection of specific DNA sequences by fluorescence amplification: a color complementation assay.

1989 • 186 citations

Specificity of the nick-closing activity of bacteriophage T4 DNA ligase

1989 • 163 citations

Molecular cloning of the DNA ligase gene from bacteriophage T4

1979 • 109 citations

[6] Oligonucleotide hybridization techniques

1987 • 95 citations

Thermophilic DNA ligase. Purification and properties of the enzyme from Thermus thermophilus HB8.

1984 • 79 citations

[16] An automated DNA synthesizer employing deoxynucleoside 3′-phosphoramidites

1987 • 59 citations

Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli

1986 • 56 citations

A strategy for high-volume sequencing of cosmid DNAs: random and directed priming with a library of oligonucleotides.

1989 • 55 citations

Blunt-end and single-strand ligations by Escherichia coli ligase: influence on an in vitro amplication scheme

1990 • 38 citations

Mutation detection using nucleotide analogs that alter electrophoretic mobility

1989 • 31 citations