Design and gene delivery activity of modified polyethylenimines

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine.

1995 • 6,103 citations

Encyclopedia of polymer science and engineering

1985 • 5,821 citations

Direct Gene Transfer into Mouse Muscle in Vivo

1990 • 3,985 citations

Regulation of transport pathways in tumor vessels: Role of tumor type and microenvironment

1998 • 2,375 citations

Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA

1999 • 1,702 citations

Sterically stabilized liposomes: improvements in pharmacokinetics and antitumor therapeutic efficacy.

1991 • 1,591 citations

Cellular and Molecular Barriers to Gene Transfer by a Cationic Lipid

1995 • 1,406 citations

Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations.

1994 • 1,306 citations

Polyamidoamine cascade polymers mediate efficient transfection of cells in culture

1993 • 1,186 citations

Poly(ethylenimine) and its role in gene delivery

1999 • 1,182 citations

PEGylated DNA/transferrin–PEI complexes: reduced interaction with blood components, extended circulation in blood and potential for systemic gene delivery

1999 • 1,165 citations

Efficient transfer of genetic material into mammalian cells using Starburst polyamidoamine dendrimers.

1996 • 995 citations

High Levels of Foreign Gene Expression in Hepatocytes after Tail Vein Injections of Naked Plasmid DNA

1999 • 934 citations

Direct gene transfer with DNA-liposome complexes in melanoma: expression, biologic activity, and lack of toxicity in humans.

1993 • 888 citations

Receptor-mediated in vitro gene transformation by a soluble DNA carrier system.

1987 • 841 citations

Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery

1999 • 819 citations

In Vitro Gene Delivery by Degraded Polyamidoamine Dendrimers

1996 • 805 citations

The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes

1997 • 798 citations

Evidence for the role of proteoglycans in cation-mediated gene transfer.

1996 • 765 citations

Gene delivery: A single nuclear localization signal peptide is sufficient to carry DNA to the cell nucleus

1999 • 718 citations

Polyethylenimine but Not Cationic Lipids Promotes Transgene Delivery to the Nucleus in Mammalian Cells

1998 • 711 citations

Different behavior of branched and linear polyethylenimine for gene deliveryin vitro andin vivo

2001 • 677 citations

The influence of endosome-disruptive peptides on gene transfer using synthetic virus-like gene transfer systems.

1994 • 668 citations

Transferrin-polycation conjugates as carriers for DNA uptake into cells.

1990 • 657 citations

Receptor-mediated gene delivery and expression in vivo.

1988 • 642 citations

CpG DNA: A potent signal for growth, activation, and maturation of human dendritic cells

1999 • 638 citations

Metabolic instability of plasmid DNA in the cytosol: a potential barrier to gene transfer

1999 • 619 citations

In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment.

1990 • 603 citations

Microvascular permeability of normal and neoplastic tissues

1986 • 601 citations

Activation of the Complement System by Synthetic DNA Complexes: A Potential Barrier for Intravenous Gene Delivery

1996 • 583 citations

The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells

1998 • 568 citations

A Powerful Nonviral Vector for In Vivo Gene Transfer into the Adult Mammalian Brain: Polyethylenimine

1996 • 544 citations

Cell cycle dependence of gene transfer by lipoplex, polyplex and recombinant adenovirus

2000 • 524 citations

Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes.

1992 • 483 citations

Nonviral Gene Therapy: The Promise of Genes as Pharmaceutical Products

1995 • 471 citations

In vivo gene transfer via intravenous administration of cationic lipid–protamine–DNA (LPD) complexes

1997 • 470 citations

Adenovirus enhancement of transferrin-polylysine-mediated gene delivery.

1991 • 431 citations

In vivo gene electroinjection and expression in rat liver

1996 • 431 citations

Nanoscopic structure of DNA condensed for gene delivery

1997 • 405 citations

Factors influencing the efficiency of cationic liposome-mediated intravenous gene delivery

1997 • 403 citations

Electropermeabilization of skeletal muscle enhances gene transfer in vivo

1999 • 383 citations

Factors affecting blood clearance and in vivo distribution of polyelectrolyte complexes for gene delivery

1999 • 381 citations

Ionization of Poly(ethylenimine) and Poly(allylamine) at Various pH′s

1994 • 377 citations

Targeting gene expression to hypoxic tumor cells

1997 • 373 citations

A peptide nucleic acid–nuclear localization signal fusion that mediates nuclear transport of DNA

1999 • 373 citations

Polyethylenimine-based intravenous delivery of transgenes to mouse lung

1998 • 367 citations

Gene Transfer with Synthetic Cationic Amphiphiles: Prospects for Gene Therapy

1994 • 366 citations

Characterization of Vectors for Gene Therapy Formed by Self-Assembly of DNA with Synthetic Block Co-Polymers

1996 • 365 citations

Coupling of cell-binding ligands to polyethylenimine for targeted gene delivery

1997 • 363 citations

Polycation-based DNA complexes for tumor-targeted gene deliveryin vivo

1999 • 351 citations

Polyethylenimine/DNA complexes shielded by transferrin target gene expression to tumors after systemic application

2001 • 350 citations

Evaluation of polyether-polyethyleneimine graft copolymers as gene transfer agents

2000 • 349 citations

Optimized galenics improve in vitro gene transfer with cationic molecules up to 1000-fold.

1996 • 348 citations

Interactions of polymeric and liposomal gene delivery systems with extracellular glycosaminoglycans: physicochemical and transfection studies

1999 • 342 citations

Transferrin-polycation-mediated introduction of DNA into human leukemic cells: stimulation by agents that affect the survival of transfected DNA or modulate transferrin receptor levels.

1990 • 340 citations

In Vitro Gene Delivery to Hepatocytes with Galactosylated Polyethylenimine

1997 • 339 citations

Gene transfer into hepatocytes using asialoglycoprotein receptor mediated endocytosis of DNA complexed with an artificial tetra-antennary galactose ligand

1992 • 325 citations

Immunotherapy of malignancy by in vivo gene transfer into tumors.

1993 • 320 citations

Stabilization of cationic liposome‐plasmid DNA complexes by polyamines and poly(ethylene glycol)‐phospholipid conjugates for efficient in vivo gene delivery

1997 • 316 citations

Size, diffusibility and transfection performance of linear PEI/DNA complexes in the mouse central nervous system

1998 • 313 citations

Systemic linear polyethylenimine (L-PEI)-mediated gene delivery in the mouse

2000 • 312 citations

An electron microscopy study into the mechanism of gene transfer with lipopolyamines.

1996 • 301 citations

Characterization of Cationic Liposome-Mediated Gene TransferIn Vivoby Intravenous Administration

1997 • 293 citations

Liposomes as drug carriers : recent trends and progress

1988 • 285 citations

Transfection and physical properties of various saccharide, poly(ethylene glycol), and antibody-derivatized polyethylenimines (PEI)

1999 • 280 citations

Delivery of drugs, proteins and genes into cells using transferrin as a ligand for receptor-mediated endocytosis

1994 • 278 citations

DNA vector chemistry: The covalent attachment of signal peptides to plasmid DNA

1998 • 271 citations

Complexes of Adenovirus with Polycationic Polymers and Cationic Lipids Increase the Efficiency of Gene Transfer in Vitro and in Vivo

1997 • 268 citations

ExGen 500 is an efficient vector for gene delivery to lung epithelial cells in vitro and in vivo

1997 • 253 citations

Factors controlling the efficiency of cationic lipid-mediated transfection in vivo via intravenous administration

1997 • 249 citations

Novel vectors for gene delivery formed by self-assembly of DNA with poly(l-lysine) grafted with hydrophilic polymers

1998 • 247 citations

Different Strategies for Formation of PEGylated EGF-Conjugated PEI/DNA Complexes for Targeted Gene Delivery

2001 • 243 citations

Gene transfer with synthetic virus-like particles via the integrin-mediated endocytosis pathway

1999 • 236 citations

CpG DNA and LPS induce distinct patterns of activation in human monocytes

1999 • 210 citations

In Vivo Delivery to Tumors of DNA Complexed with Linear Polyethylenimine

1999 • 209 citations

A combination of poloxamers increases gene expression of plasmid DNA in skeletal muscle

2000 • 209 citations

Expression of naked DNA in human, pig, and mouse skin.

1996 • 198 citations

Organ-specific endothelial cell uptake of cationic liposome-DNA complexes in mice

1997 • 198 citations

Cell-specific nuclear import of plasmid DNA

1999 • 193 citations

In vitro and in vivo targeting of gene expression to melanoma cells.

1993 • 190 citations

Nonviral Gene Delivery to the Rat Kidney with Polyethylenimine

1997 • 186 citations

The efficient expression of intravascularly delivered DNA in rat muscle

1998 • 166 citations

Biological activity of oligonucleotide-poly(L-lysine) conjugates: mechanism of cell uptake

1990 • 160 citations

Gene Transfer into Mammalian Cells Using Histone-Condensed Plasmid DNA

1996 • 158 citations

Comparison between cationic polymers and lipids in mediating systemic gene delivery to the lungs

1999 • 157 citations

Decreased Binding to Proteins and Cells of Polymeric Gene Delivery Vectors Surface Modified with a Multivalent Hydrophilic Polymer and Retargeting through Attachment of Transferrin

2000 • 157 citations

Physicochemical and Pharmacokinetic Characteristics of Plasmici DNA/ Cationic Liposome Complexes

1995 • 155 citations

Targeting DNA to Cells with Basic Fibroblast Growth Factor (FGF2)

1996 • 153 citations

Gene transfer into the airway epithelium of animals by targeting the polymeric immunoglobulin receptor.

1995 • 151 citations

Protective copolymers for nonviral gene vectors: synthesis, vector characterization and application in gene delivery

2000 • 148 citations

Rapid crossing of the pulmonary endothelial barrier by polyethylenimine/DNA complexes

2000 • 140 citations

Nonviral vectors for gene therapy

1999 • 139 citations

[42] Receptor-mediated transport of DNA into eukaryotic cells

1993 • 115 citations

Receptor-Mediated Gene Transfer into Human T LymphocytesviaBinding of DNA/CD3 Antibody Particles to the CD3 T Cell Receptor Complex

1995 • 110 citations

A novel gene delivery system using EGF receptor‐mediated endocytosis

1994 • 102 citations

Efficient Gene Delivery into Human Dendritic Cells by Adenovirus Polyethylenimine and Mannose Polyethylenimine Transfection

1999 • 98 citations

Epidermal growth factor mediated DNA delivery into lung cancer cells via the epidermal growth factor receptor.

1996 • 90 citations

DirectIn VivoGene Transfer to Airway Epithelium Employing Adenovirus–Polylysine–DNA Complexes

1993 • 85 citations

Rhinovirus-mediated endosomal release of transfection complexes

1995 • 85 citations

Interaction of liposomal and polycationic transfection complexes with pulmonary surfactant

1999 • 81 citations

Glycerol Enhancement of Ligand-Polylysine/DNA Transfection

1996 • 69 citations

Bacterial DNA is implicated in the inflammatory response to delivery of DNA/DOTAP to mouse lungs

2000 • 63 citations

Delivery of bacterial artificial chromosomes into mammalian cells with psoralen-inactivated adenovirus carrier

1997 • 49 citations

Obstacles and advances in non-viral gene delivery.

2000 • 48 citations

Mechanism of polycation stimulation of pinocytosis

1979 • 39 citations

Development of Transferrin-Polycation/DNA Based Vectors for Gene Delivery to Melanoma Cells

1999 • 34 citations

Immunologic responses to gene transfer into mice via the polymeric immunoglobulin receptor.

1996 • 31 citations

Cytokine gene-modified tumor cells for prophylactic and therapeutic vaccination: IL-2, IFN-gamma, or combination IL-2 + IFN-gamma.

1998 • 29 citations

Polycation/DNA complexes for in vivo gene delivery

2000 • 29 citations

Polyethylenimines

1999 • 27 citations

Gene delivery into malignant cells in vivo by a conjugated adenovirus/DNA complex.

1997 • 14 citations

Determination of a first‐order rate constant by difference gel chromatography: Dissociation of insulin by alkali

1974 • 2 citations