Two‐dimensional electrophoresis of human placental mitochondria and protein identification by mass spectrometry: Toward a human mitochondrial proteome

Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels

1996 • 8,994 citations

Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels

1987 • 4,186 citations

Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polycrylamide to nitrocellulose

1984 • 2,553 citations

Oxidative damage and mitochondrial decay in aging.

1994 • 2,024 citations

Mitochondrial DNA damage is more extensive and persists longer than nuclear DNA damage in human cells following oxidative stress

1997 • 1,762 citations

Mitochondrial control of apoptosis

1997 • 1,500 citations

In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis

1992 • 1,259 citations

Progress with gene‐product mapping of the Mollicutes: Mycoplasma genitalium

1995 • 934 citations

The NADH:ubiquinone oxidoreductase (complex I) of respiratory chains

1992 • 772 citations

Improvement of the solubilization of proteins in two‐dimensional electrophoresis with immobilized pH gradients

1997 • 484 citations

Cloning and Expression of a Novel Mammalian Thioredoxin

1997 • 391 citations

Mass spectrometric approaches for the identification of gel‐separated proteins

1995 • 378 citations

Sample application by in‐gel rehydration improves the resolution of two‐dimensional electrophoresis with immobilized pH gradients in the first dimension

1994 • 367 citations

Immobilized pH Gradients: Theory and Methodology

1990 • 363 citations

Delayed Extraction Improves Specificity in Database Searches by Matrix-assisted Laser Desorption/Ionization Peptide Maps

1996 • 297 citations

Has the endosymbiont hypothesis been proven?

1982 • 281 citations

Elimination of point streaking on silver stained two‐dimensional gels by addition of iodoacetamide to the equilibration buffer

1987 • 271 citations

A comparison between low background silver diammine and silver nitrate protein stains

1992 • 266 citations

Sensitive colloidal metal (gold or silver) staining of protein blots on nitrocellulose membranes

1985 • 242 citations

Identification of mouse liver proteins on two‐dimensional electrophoresis gels by matrix‐assisted laser desorption/ionization mass spectrometry ofin situenzymatic digests

1997 • 231 citations

Identification of the subunits of F1F0-ATPase from bovine heart mitochondria

1991 • 200 citations

Micropreparative two‐dimensional electrophoresis allowing the separation of samples containing milligram amounts of proteins

1993 • 191 citations

Interlaboratory reproducibility of yeast protein patterns analyzed by immobilized pH gradient two‐dimensional gel electrophoresis

1995 • 152 citations

Two‐dimensional electrophoresis of membrane proteins: A current challenge for immobilized pH gradients

1997 • 138 citations

The REF52 Protein Database

1989 • 131 citations

Cross‐species identification of proteins separated by two‐dimensional gel electrophoresis using matrix‐assisted laser desorption ionisation/time‐of‐flight mass spectrometry and amino acid composition

1995 • 127 citations

Experimentally improved reliability of ultrasensitive silver staining of protein in polyacrylamide gels

1982 • 117 citations

Oxidative Phosphorylation Diseases and Mitochondrial DNA Mutations: Diagnosis and Treatment

1994 • 112 citations

Imidazole‐SDS‐Zn reverse staining of proteins in gels containing or not SDS and microsequence of individual unmodified electroblotted proteins

1992 • 107 citations

Polyvinylpyrrolidone as a Blocking Agent in Immunochemical Studies

1993 • 95 citations

A Semidry Electroblotting System Efficiently Transfers Both High- and Low-Molecular-Weight Proteins Separated by SDS-PAGE

1993 • 84 citations

How many proteins are there in a typical mammalian cell?

1982 • 80 citations

Highly resolving two-dimensional gels for protein sequencing.

1991 • 79 citations

Animal and plant mitochondria contain specific thioredoxins

1989 • 76 citations

Comparison of in‐gel and on‐membrane digestion methods at low to sub‐pmol level for subsequent peptide and fragment‐ion mass analysis using matrix‐assisted laser‐desorption/ionization mass spectrometry

1997 • 74 citations

Proteome Analysis: Genomics via the Output Rather Than the Input Code

1997 • 65 citations

Advantages of separations on "giant" two-dimensional gels for detection of physiologically relevant changes in the expression of protein gene-products.

1984 • 63 citations

Rapid electrotransfer of proteins from polyacrylamide gel to nitrocellulose membrane using surface-conductive glass as anode

1985 • 56 citations

A Permanent Zn2+ Reverse Staining Method for the Detection and Quantification of Proteins in Polyacrylamide Gels

1993 • 47 citations

A Nonradioactive Double Detection Method for the Assignment of Spots in Two-Dimensional Blots

1997 • 37 citations

Photographic Processing Chemistry

1966 • 30 citations

Silver staining of histones in Triton-acid-urea gels

1983 • 24 citations

Stage transitions in B-lymphocyte differentiation correlate with limited variations in nuclear proteins.

1991 • 16 citations

Immobilized pH 2.5–11 gradients for two‐dimensional electrophoresis

1992 • 15 citations

High-resolution two-dimensional electrophoresis of nuclear proteins: A comparison of HeLa nuclei prepared by three different methods

1992 • 15 citations

[11] Purification of mitochondrial DNA from human cell cultures and placenta

1996 • 15 citations

The rat liver epithelial (RLE) cell nuclear protein database

1993 • 14 citations