EcoRV restriction endonuclease: communication between DNA recognition and catalysis

DNA sequencing with chain-terminating inhibitors

1977 • 69,181 citations

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

1985 • 15,222 citations

A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots

1984 • 2,189 citations

A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system

1981 • 1,991 citations

Trans-complementable copy-number mutants of plasmid ColE1

1980 • 975 citations

Binding Energy, Specificity, and Enzymic Catalysis: The Circe Effect

1975 • 844 citations

The hydrogen bond in molecular recognition

1987 • 462 citations

Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication

1983 • 362 citations

An improved filamentous helper phage for generating single-stranded plasmid DNA

1986 • 348 citations

The Energetic Basis of Specificity in the Eco RI Endonuclease—DNA Interaction

1990 • 327 citations

Efficient oligonucleotide-directed construction of mutations in expression vectors by the gapped duplex DNA method using alternating selectable markers

1989 • 321 citations

On the specificity of DNA-protein interactions.

1986 • 308 citations

Molecular cloning of the DNA ligase gene from bacteriophage T4

1979 • 171 citations

Effects of functional group changes in the EcoRI recognition site on the cleavage reaction catalyzed by the endonuclease

1987 • 169 citations

Construction of a plasmid that overproduces the large proteolytic fragment (Klenow fragment) of DNA polymerase I of Escherichia coli.

1983 • 168 citations

EcoRV restriction endonuclease binds all DNA sequences with equal affinity

1991 • 139 citations

Characterization of the genes coding for theEcoRV restriction and modification system ofEscherichia coli

1984 • 135 citations

Structure and function of restriction endonucleases

1991 • 132 citations

Discrimination between DNA sequences by the EcoRV restriction endonuclease

1989 • 124 citations

Modes of DNA cleavage by the EcoRV restriction endonuclease

1988 • 83 citations

Site-directed mutagenesis studies with EcoRV restriction endonuclease to identify regions involved in recognition and catalysis

1991 • 81 citations

Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymic activity, not, however, the preference of this restriction endonuclease for cleavage within the site -GAATTC-

1989 • 76 citations

Structure and function of restriction endonucleases

1992 • 73 citations

Interaction of the EcoRV restriction endonuclease with the deoxyadenosine and thymidine bases in its recognition hexamer d(GATATC)

1990 • 67 citations

The cleavage site for the restriction endonuclease EcoRV is 5′-GAT/ATC-3′

1984 • 60 citations

Analysis of the recognition mechanism involved in the EcoRV catalyzed cleavage of DNA using modified oligodeoxynucleotides

1988 • 55 citations

Substrate recognition by the EcoRI endonuclease

1990 • 55 citations

Overproduction of theEcoR V endonuclease and methylase

1985 • 42 citations

Fidelity of DNA recognition by the EcoRV restriction/modification system in vivo

1990 • 41 citations

Nomenclature for bacterial genes coding for class-II restriction endonucleases and modification methyltransferases

1988 • 39 citations

Determinants of EcoRI endonuclease sequence discrimination.

1989 • 36 citations

The activity of the EcoRV restriction endonuclease is influenced by flanking DNA sequences both inside and outside the DNA-protein complex

1992 • 36 citations

Relaxed specificity of the EcoRV restriction endonuclease

1986 • 32 citations

Crystallization of complexes of EcoRV endonuclease with cognate and non-cognate DNA fragments

1991 • 20 citations

Probing the role of glutamic acid 144 in the EcoRI endonuclease using aspartic acid and glutamine replacements.

1990 • 18 citations

Cofactor requirements of BamHI mutant endonuclease E77K and its suppressor mutants

1991 • 17 citations