Effect of disulfide bridge formation on the NMR spectrum of a protein: Studies on oxidized and reduced Escherichia coli thioredoxin

Relationship between nuclear magnetic resonance chemical shift and protein secondary structure

1991 • 1,888 citations

THIOREDOXIN

1985 • 1,885 citations

Thioredoxin and Glutaredoxin Systems

1989 • 1,778 citations

Rapid recording of 2D NMR spectra without phase cycling. Application to the study of hydrogen exchange in proteins

1989 • 1,395 citations

Sensitivity-enhanced two-dimensional heteronuclear shift correlation NMR spectroscopy

1986 • 1,342 citations

Reduction of Ribonucleotides

1979 • 1,170 citations

Empirical correlation between protein backbone conformation and C.alpha. and C.beta. 13C nuclear magnetic resonance chemical shifts

1991 • 1,072 citations

Enzymatic Synthesis of Deoxyribonucleotides

1964 • 917 citations

Thioredoxin catalyzes the reduction of insulin disulfides by dithiothreitol and dihydrolipoamide.

1979 • 907 citations

Overcoming the overlap problem in the assignment of proton NMR spectra of larger proteins by use of three-dimensional heteronuclear proton-nitrogen-15 Hartmann-Hahn-multiple quantum coherence and nuclear Overhauser-multiple quantum coherence spectroscopy: application to interleukin 1.beta.

1989 • 840 citations

Sequence of protein disulphide isomerase and implications of its relationship to thioredoxin

1985 • 622 citations

1H1H correlation via isotropic mixing of 13C magnetization, a new three-dimensional approach for assigning 1H and 13C spectra of 13C-enriched proteins

1990 • 580 citations

Crystal structure of thioredoxin from Escherichia coli at 1.68 Å resolution

1990 • 568 citations

Two-dimensional correlation of connected NMR transitions

1985 • 478 citations

Three-dimensional structure of Escherichia coli thioredoxin-S2 to 2.8 A resolution.

1975 • 435 citations

Nmr studies of the rates of proline cis–trans isomerization in oligopeptides

1981 • 368 citations

Differential reactivity of the functional sulfhydryl groups of cysteine-32 and cysteine-35 present in the reduced form of thioredoxin from Escherichia coli.

1980 • 364 citations

Structural and functional relations among thioredoxins of different species

1991 • 363 citations

Enzymatic Synthesis of Deoxyribonucleotides

1964 • 340 citations

Reduction of disulfides by thioredoxin. Exceptional reactivity of insulin and suggested functions of thioredoxin in mechanism of hormone action.

1979 • 324 citations

A new analysis of proton chemical shifts in proteins

1991 • 287 citations

Theory of the time dependent transferred nuclear Overhauser effect: Applications to structural analysis of ligand-protein complexes in solution

1983 • 285 citations

Carbon-13 magnetic resonance spectroscopy. Spectrum of proline in oligopeptides

1973 • 279 citations

The X‐Pro peptide bond as an nmr probe for conformational studies of flexible linear peptides

1976 • 270 citations

Escherichia coli thioredoxin: a subunit of bacteriophage T7 DNA polymerase.

1976 • 267 citations

Mimicking the active site of protein disulfide-isomerase by substitution of proline 34 in Escherichia coli thioredoxin

1991 • 256 citations

The role of thioredoxin in filamentous phage assembly. Construction, isolation, and characterization of mutant thioredoxins.

1986 • 223 citations

Carbon‐13 NMR chemical shifts of the common amino acid residues measured in aqueous solutions of the linear tetrapeptides H‐Gly‐Gly‐ X‐<scp>L</scp>‐ Ala‐OH

1978 • 213 citations

Carbon-13-NMR of peptides and proteins

1978 • 189 citations

A Specific Enzymatic Reduction of l(-) Methionine Sulfoxide and a Related Nonspecific Reduction of Disulfides

1960 • 173 citations

Yeast Sulfate-reducing System

1961 • 160 citations

Three-dimensional solution structure of the reduced form of Escherichia coli thioredoxin determined by nuclear magnetic resonance spectroscopy

1990 • 160 citations

Production of platelet-activating factor by chick retina.

1986 • 157 citations

Two-, Three-, and Four-Dimensional NMR Methods for Obtaining Larger and More Precise Three-Dimensional Structures of Proteins in Solution

1991 • 151 citations

Interaction of mutant thioredoxins of Escherichia coli with the gene 5 protein of phage T7. The redox capacity of thioredoxin is not required for stimulation of DNA polymerase activity.

1986 • 146 citations

Enzymatic reduction-oxidation of protein disulfides by thioredoxin

1984 • 145 citations

High-resolution three-dimensional structure of reduced recombinant human thioredoxin in solution

1991 • 141 citations

Accurate measurements of long-range heteronuclear coupling constants from homonuclear 2D NMR spectra of isotope-enriched proteins

1989 • 136 citations

Improved three-dimensional1H−13C−1H correlation spectroscopy of a13C-labeled protein using constant-time evolution

1991 • 130 citations

Nmr studies of the molecular conformations in the linear oligopeptides H‐(<scp>L</scp>‐Ala)_n‐<scp>L</scp>‐Pro‐OH

1976 • 129 citations

NMR structure of oxidized Escherichia coli glutaredoxin: Comparison with reduced E. coli glutaredoxin and functionally related proteins

1992 • 121 citations

Comparison of backbone and tryptophan side-chain dynamics of reduced and oxidized Escherichia coli thioredoxin using nitrogen-15 NMR relaxation measurements

1993 • 119 citations

The conserved, buried aspartic acid in oxidized Escherichia coli thioredoxin has a pKa of 7.5. Its titration produces a related shift in global stability

1991 • 111 citations

A Pro to His mutation in active site of thioredoxin increases its disulfide-isomerase activity 10-fold. New refolding systems for reduced or randomly oxidized ribonuclease.

1992 • 110 citations

Stereospecific assignment of β-methylene protons in larger proteins using 3D15N-separated Hartmann-Hahn and13C-separated rotating frame Overhauser spectroscopy

1991 • 105 citations

Observation of 1000-fold enhancement of nitrogen-15 NMR via proton-detected multiquantum coherences: studies of large peptides

1984 • 99 citations

Sequence-specific1H n.m.r. assignments and determination of the three-dimensional structure of reduced escherichia coli glutaredoxin

1991 • 96 citations

Assignment of the proton NMR spectrum of reduced and oxidized thioredoxin: sequence-specific assignments, secondary structure, and global fold

1989 • 88 citations

Determination of the pKa values of active-center cysteines, cysteines-32 and -35, in Escherichia coli thioredoxin by Raman spectroscopy

1993 • 80 citations

Enzymatic reduction of disulfide bonds in fibrin-o gen by the thioredoxin system I. Identification of reduced bonds and studies on reoxidation process

1974 • 77 citations

Manifestation in the ¹³C‐NMR spectra of two different molecular conformations of a cyclic pentapeptide

1972 • 75 citations

New nuclear magnetic resonance experiment for measurements of the vicinal coupling constants 3JHN.alpha. in proteins

1990 • 73 citations

A novel approach for studies of the molecular conformations in flexible polypeptides

1974 • 68 citations

Proton-transfer effects in the active-site region of Escherichia coli thioredoxin using two-dimensional proton NMR

1991 • 64 citations

Three-dimensional structure of thioredoxin induced by bacteriophage T4.

1978 • 61 citations

T7-induced DNA polymerase. Requirement for thioredoxin sulfhydryl groups.

1983 • 60 citations

Transforming growth factor .beta.1: Secondary structure as determined by heteronuclear magnetic resonance spectroscopy

1993 • 59 citations

Identification of a Thioredoxin Induced by Bacteriophage T4

1969 • 51 citations

Escherichia coli thioredoxin folds into two compact forms of different stability to urea denaturation

1989 • 48 citations

Assignment of the aliphatic proton and carbon-13 resonances of the Bacillus subtilis glucose permease IIA domain using double- and triple-resonance heteronuclear three-dimensional NMR spectroscopy

1992 • 46 citations

A mutant thioredoxin from Escherichia coli tsnC 7007 that is nonfunctional as subunit of phage T7 DNA polymerase.

1981 • 42 citations

Assignment of the ¹⁵N NMR spectra of reduced and oxidized Escherichia coli thioredoxin

1991 • 41 citations

Structural differences between oxidized and reduced thioredoxin monitored by two‐dimensional ¹H NMR spectroscopy

1988 • 38 citations

Characterization of bacteriophage T7 DNA polymerase purified to homogeneity by antithioredoxin immunoadsorbent chromatography.

1981 • 37 citations

Crystallization and preliminary crystallographic data for thioredoxin from Escherichia coli B

1970 • 37 citations

Measurement of small heteronuclear 1H15N coupling constants in 15N-labeled proteins by 3D HNNHAB-COSY

1991 • 33 citations

Structural comparison between oxidized and reduced Escherichia coli thioredoxin. Proton NMR and CD studies

1988 • 32 citations

Nuclear magnetic resonance studies of redox‐induced conformational changes in thioredoxin from Escherichia coli

1976 • 32 citations

Proline assignments and identification of the cis K116/P117 peptide bond in liganded staphylococcal nuclease using isotope edited 2D NMR spectroscopy

1989 • 27 citations

Bacteriophage T7 DNA polymerase: cloning and high-level expression.

1985 • 18 citations

Nuclear magnetic resonance studies of recombinant Escherichia coli glutaredoxin

1991 • 17 citations

Thioredoxin reductase-dependent insulin disulfide reduction by phage T7 DNA polymerase reflects dissociation of the enzyme into subunits

1989 • 13 citations