Escherichia coli thioredoxin folds into two compact forms of different stability to urea denaturation

[14]Determination and analysis of urea and guanidine hydrochloride denaturation curves

1986 • 2,265 citations

Protein denaturation. C. Theoretical models for the mechanism of denaturation.

1970 • 1,550 citations

[8] Reassessment of Ellman's reagent

1983 • 1,076 citations

Enzymatic Synthesis of Deoxyribonucleotides

1964 • 917 citations

Kanamycin-resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8 and pEMBL9

1986 • 444 citations

Three-dimensional structure of Escherichia coli thioredoxin-S2 to 2.8 A resolution.

1975 • 435 citations

NMR sequential assignment of Escherichia coli thioredoxin utilizing random fractional deuteriation

1988 • 274 citations

Escherichia coli thioredoxin: a subunit of bacteriophage T7 DNA polymerase.

1976 • 267 citations

Electrophoretic analysis of the unfolding of proteins by urea

1979 • 241 citations

The role of thioredoxin in filamentous phage assembly. Construction, isolation, and characterization of mutant thioredoxins.

1986 • 223 citations

Proton-nitrogen-15 heteronuclear NMR studies of Escherichia coli thioredoxin in samples isotopically labeled by residue type

1985 • 212 citations

Conformational and functional similarities between glutaredoxin and thioredoxins.

1984 • 205 citations

Interaction of mutant thioredoxins of Escherichia coli with the gene 5 protein of phage T7. The redox capacity of thioredoxin is not required for stimulation of DNA polymerase activity.

1986 • 146 citations

Thioredoxin-catalyzed refolding of disulfide-containing proteins.

1986 • 137 citations

Glutaredoxin from calf thymus. Purification to homogeneity.

1982 • 133 citations

Thioredoxin is required for filamentous phage assembly.

1985 • 131 citations

Kinetic study of protein unfolding and refolding using urea gradient electrophoresis

1980 • 121 citations

Urea-induced unfolding of the .alpha. subunit of tryptophan synthase: evidence for a multistate process

1981 • 109 citations

Replacement of proline-76 with alanine eliminates the slowest kinetic phase in thioredoxin folding

1987 • 98 citations

A conformational study of thioredoxin and its tryptic fragments.

1981 • 96 citations

Sulfate-reducing pathway in Escherichia coli involving bound intermediates

1976 • 95 citations

Effect on protein stability of reversing the charge on amino groups

1982 • 89 citations

Cloning and nucleotide sequence of the trxA gene of Escherichia coli K-12

1985 • 72 citations

Thioredoxin is the bacterial protein encoded by fip that is required for filamentous bacteriophage f1 assembly

1985 • 63 citations

Conformational transitions of thioredoxin in guanidine hydrochloride

1984 • 56 citations

Equilibrium and kinetic measurements of the conformational transition of reduced thioredoxin

1987 • 56 citations

[9]Detection of folding intermediates using urea-gradient electrophoresis

1986 • 53 citations

A proton motive force transducer and its role in proton pumps, proton engines, tobacco mosaic virus assembly and hemoglobin allosterism

1982 • 51 citations

Effects of guanidine hydrochloride on the refolding kinetics of denatured thioredoxin

1986 • 39 citations

Structural differences between oxidized and reduced thioredoxin monitored by two‐dimensional ¹H NMR spectroscopy

1988 • 38 citations

Characterization of bacteriophage T7 DNA polymerase purified to homogeneity by antithioredoxin immunoadsorbent chromatography.

1981 • 37 citations

Refolding of denatured thioredoxin observed by size-exclusion chromatography

1987 • 34 citations

Structural comparison between oxidized and reduced Escherichia coli thioredoxin. Proton NMR and CD studies

1988 • 32 citations

Characterization of high-level expression and sequencing of the Escherichia coli K-12 cynS gene encoding cyanase

1987 • 31 citations

Spectroscopic characterization of thioredoxin covalently modified with monofunctional organoarsenical reagents

1987 • 26 citations

Amino acid sequence determination and three‐dimensional modelling of thioredoxin from the photosynthetic bacterium Rhodobacter sphaeroides Y

1988 • 24 citations

Equilibrium and kinetic measurements of the conformational transition of thioredoxin in urea

1986 • 17 citations