Efficient Transfection and Expression of Heterologous Genes in PC12 Cells

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1970 • 253,849 citations

Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.

1982 • 8,069 citations

Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.

1976 • 5,526 citations

Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.

1987 • 4,937 citations

A rapid, sensitive, and specific method for the determination of protein in dilute solution

1973 • 2,518 citations

Transformation of mammalian cells with genes from procaryotes and eucaryotes

1979 • 1,474 citations

Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably transforming cells with plasmid DNA.

1988 • 897 citations

Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.

1984 • 889 citations

A human beta-actin expression vector system directs high-level accumulation of antisense transcripts.

1987 • 767 citations

Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation

1985 • 738 citations

Nerve growth factor-induced neurite outgrowth in PC12 cells involves the coordinate induction of microtubule assembly and assembly-promoting factors.

1985 • 506 citations

New procedure for DNA transfection with polycation and dimethyl sulfoxide.

1984 • 481 citations

Evidence that the packaging signal of Moloney murine leukemia virus extends into the gag region

1987 • 426 citations

K-252a: a specific inhibitor of the action of nerve growth factor on PC 12 cells

1988 • 320 citations

Promoter-regulatory region of the major immediate early gene of human cytomegalovirus.

1984 • 296 citations

The effect of fibroblast growth factor on PC12 cells

1985 • 270 citations

Differential and synergistic actions of nerve growth factor and cyclic AMP in PC12 cells.

1981 • 239 citations

Regulation of a high molecular weight microtubule-associated protein in PC12 cells by nerve growth factor.

1983 • 207 citations

Protein kinase C as a component of a nerve growth factor-sensitive phosphorylation system in PC12 cells.

1986 • 197 citations

Multiple forms of the nerve growth factor protein and its subunits

1968 • 179 citations

Infection of eucaryotic cells by helper-independent recombinant adenoviruses: early region 1 is not obligatory for integration of viral DNA

1984 • 170 citations

Structure of the NGFI-A gene and detection of upstream sequences responsible for its transcriptional induction by nerve growth factor.

1989 • 164 citations

A method for increasing the sensitivity of chloramphenicol acetyltransferase assays in extracts of transfected cultured cells

1987 • 161 citations

Construction and Characterization of a Retroviral Vector Demonstrating Efficient Expression of Cloned cDNA Sequences

1988 • 157 citations

Neurite outgrowth and protein synthesis by PC12 cells as a function of substratum and nerve growth factor

1982 • 106 citations

Selective packaging of human growth hormone into synaptic vesicles in a rat neuronal (PC12) cell line.

1985 • 94 citations

Nerve growth factor increases the number of functional Na channels and induces TTX-resistant Na channels in PC12 pheochromocytoma cells

1987 • 88 citations

Nerve growth factor- and epidermal growth factor-stimulated phosphorylation of a PC12 cytoskeletally associated protein in situ.

1985 • 83 citations

Two adjacent promoter elements mediate nerve growth factor activation of the c-fos gene and bind distinct nuclear complexes.

1988 • 79 citations

Regulation of tyrosine hydroxylase and choline acetyltransferase activities by cell density in the PC12 rat pheochromocytoma clonal cell line

1979 • 68 citations

[32] Liposomes as carriers for intracellular delivery of nucleic acids

1983 • 67 citations

Poly-L-ornithine-mediated transformation of mammalian cells.

1987 • 62 citations

Amino acid sequence and distribution of mRNA encoding a major skeletal muscle laminin binding protein: an extracellular matrix-associated protein with an unusual COOH-terminal polyaspartate domain.

1988 • 53 citations

Isolation and characterization of a laminin-binding protein from rat and chick muscle.

1988 • 46 citations

Insulin-like growth factors are mitogens for rat pheochromocytoma PC 12 cells

1988 • 33 citations

Induction of Gene Expression under Human Cytomegalovirus Immediate Early Enhancer-Promoter Control by Inhibition of Protein Synthesis Is Cell Cycle-dependent

1988 • 24 citations